1887

Abstract

Effects of three mutant genes, and , on catabolite repression of mitochondrial cytochromes and the first two enzymes of haem biosynthesis were compared. The mutation gave no resistance to glucose, whereas endowed both cytochromes and 5-aminolaevulinate dehydratase with resistance, but did not alter the effect of glucose on 5-aminolaevulinate synthase. The mutation caused repression resistance of cytochromes and of the two haem biosynthetic enzymes. strains also accumulated intracellular 5-aminolaevulinate. Co-inheritance of the latter traits, sensitivity to maltose inhibition and ability to grow on raffinose in the presence of 2-deoxyglucose, demonstrated that the pleiotropic phenotype is a function of the single gene . Revertants which grew on maltose regained sensitivity to deoxyglucose and exhibited normal sensitivity of cytochromes and haem biosynthesis enzymes to repression. Addition of the mutation, which renders cytochromes resistant to repression, to a strain did not produce the same effect on 5-aminolaevulinate synthase as . It is concluded that repression patterns of haem and cytochrome biosynthesis are substantially affected by and but not by .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-5-1217
1989-05-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/5/mic-135-5-1217.html?itemId=/content/journal/micro/10.1099/00221287-135-5-1217&mimeType=html&fmt=ahah

References

  1. Arrese M., Carvajal E., Robison S., Sambunaris A., Panek A., Mattoon J. 1983; Cloning of the β-aminolevulinic acid synthase structural gene in yeast. Current Genetics 7:175–183
    [Google Scholar]
  2. Bailey R.B., Woodward A. 1984; Isolation and characterization of a pleiotropic glucose repression resistant mutant of Saccharomyces cerevisiae . Molecular and General Genetics 193:507–512
    [Google Scholar]
  3. Böker-Schmitt E., Francisci S., Schweyen R.J. 1982; Mutations releasing mitochondrial biogenesis from glucose repression in Saccharomyces cerevisiae . Journal of Bacteriology 151:303–310
    [Google Scholar]
  4. Borralho L.M., Panek A.D., Malamud D.R., Sanders H.K., Mattoon J.R. 1983; In situ assay for 5-aminolevulinate dehydratase and application to the study of a catabolite repression- resistant Saccharomyces cerevisiae mutant. Journal of Bacteriology 156:141–147
    [Google Scholar]
  5. Carlson M., Osmond B.C., Botstein D. 1981; Mutants of yeast defective in sucrose utilization. Genetics 98:25–40
    [Google Scholar]
  6. Ciriacy M. 1978; A yeast mutant with glucose resistant formation of mitochondrial enzymes. Molecular and General Genetics 159:329–335
    [Google Scholar]
  7. Entian K.-D. 1977; Lack of carbon catabolite inactivation in a mutant of Saccharomyces cerevisiae with reduced hexokinase activity. Molecular and General Genetics 158:201–210
    [Google Scholar]
  8. Entian K.-D. 1980; A defect in carbon catabolite repression associated with uncontrollable and excessive maltose uptake. Molecular and General Genetics 179:169–175
    [Google Scholar]
  9. Entian K.-D. 1986; Glucose repression: a complex regulatory system in yeast. Microbiological Sciences 3:366–371
    [Google Scholar]
  10. Entian K.-D., Zimmermann F.K. 1980; Glycoltic enzymes and intermediates in carbon catabolite repression mutants of Saccharomyces cerevisiae . Molecular and General Genetics 177:345–350
    [Google Scholar]
  11. Entian K.-D., Zimmermann F.K., Scheel I. 1977; A partial defect in carbon catabolite repression in mutants of Saccharomyces cerevisiae with reduced hexose phosphorylation. Molecular and General Genetics 156:99–105
    [Google Scholar]
  12. Gancedo J.M., Gancedo C. 1971; Fructose-1,6- diphosphatase, phosphofructokinase and glucose-6- phosphate dehydrogenase from fermenting and nonfermenting yeasts. Archives of Microbiology 76:132–138
    [Google Scholar]
  13. Gancedo J.M., Gancedo C. 1986; Catabolite repression mutants of yeast. FEMS Microbiology Reviews 32:179–187
    [Google Scholar]
  14. Jayaraman J., Padmanaban G., Malathi K., Sarma P.S. 1971; Haem synthesis during mito- chondrogenesis in yeast. Biochemical Journal 121:531–535
    [Google Scholar]
  15. Labbe-Bois R., Volland C. 1977; Changes in the, activities of the protoheme-synthesizing systems during growth of yeast under different conditions. Archives of Biochemistry and Biophysics 179:565–577
    [Google Scholar]
  16. Lobo Z., Maitra P.K. 1977; Genetics of yeast hexokinase. Genetics 86:726–744
    [Google Scholar]
  17. Mahler H.R., Lin C.C. 1978; Exogenous adenosine 3',5'-monophosphate can release yeast from catabolite repression. Biochemical and Biophysical Research Communications 83:1039–1047
    [Google Scholar]
  18. Malamud D.R., Borralho L.M., Panek A.D., Mattoon J.R. 1979; Modulation of cytochrome biosynthesis in yeast by antimetabolite action of levulinic acid. Journal of Bacteriology 138:799–804
    [Google Scholar]
  19. Malamud D.R., Padrao G.R.B., Borralho L., Arrese M., Panek A.D., Mattoon J.R. 1983; Regulation of porphyrin biosynthesis in yeast. Use of 5-aminolevulinic acid in characterizing in vivo effects of mutation. Brazilian Journal of Medical and Biological Research 16:203–213
    [Google Scholar]
  20. Matsumoto K., Uno I., Toh-E A., Ishikawa T., Oshima Y. 1982; Cyclic AMP may not be involved in catabolite repression in Saccharomyces cerevisiae. Evidence from mutants capable of utilizing it as an adenine source. Journal of Bacteriology 150:277–285
    [Google Scholar]
  21. Matsumoto K., Yoshimatsu T., Oshima Y. 1983; Recessive mutations conferring resistance to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae . Journal of Bacteriology 153:1405–1414
    [Google Scholar]
  22. Mattoon J.R., Malamud D.R., Brunner A., Braz G., Carvajal E., Lancashire W.E., Panek A.D. 1978; Regulation of heme formation and cytochrome biosynthesis in normal and mutant yeasts. In Biochemistry and Genetics of Yeast, Pure and Applied Aspects pp. 145–160 Bacila M., Horecker B., Stoppani A. O.M. Edited by New York: Academic Press;
    [Google Scholar]
  23. Mattoon J.R., Lancashire W.E., Sanders H.K., Carvajal E., Malamud D.R., Braz G.R.C., Panek A.D. 1979; Oxygen and catabolite regulation of hemoprotein biosynthesis in yeast. In Biochemical and Clinical Aspects of Oxygen pp. Caughey W. Edited by New York: Academic Press;
    [Google Scholar]
  24. Mauzerall D., Granick S. 1956; The occurrence and determination of 5-aminolevulinic acid and porphobilinogen in urine. Journal of Biological Chemistry 219:435–446
    [Google Scholar]
  25. Michels C.A., Romanowski A;. 1980; Pleiotropic glucose repression-resistant mutation in Saccharomyces carlsbergensis . Journal of Bacteriology 143:674–679
    [Google Scholar]
  26. Michels C.A., Hahnenberger K.M., Sylvestre Y. 1983; Pleiotropic mutations regulating resistance to glucose repression in Saccharomyces carlsbergensis are allelic to the structural gene for hexokinase B. Journal of Bacteriology 153:574–578
    [Google Scholar]
  27. Mortimer R.K., Hawthorne D.C. 1969; Yeast genetics. In The Yeasts 1 pp. 385–460 Rose A.H., Harrison J.S. Edited by New York: Academic Press;
    [Google Scholar]
  28. Paschoalin V.M.F., Costa-Carvalho V.L.A., Panek A.D. 1986; Further evidence for the alternative pathway of trehalose synthesis linked to maltose utilization in Saccharomyces . Current Genetics 10:725–731
    [Google Scholar]
  29. Perlman P.S., Mahler H.R. 1974; Derepression of mitochondria and their enzymes in yeast: regulatory aspects. Archives of Biochemistry and Biophysics 162:248–271
    [Google Scholar]
  30. Polakis E.S., Bartley W. 1965; Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources. Biochemical Journal 97:284–297
    [Google Scholar]
  31. Pretlow T.P., Sherman F. 1967; Porphyrins and zinc porphyrins in normal and mutant strains of yeast. Biochimica et biophysica acta 148:629–644
    [Google Scholar]
  32. Van Rijn J., Van Wijk R. 1972; Differential sensitivities of the two malate dehydrogenases and the maltose permease to the effect of glucose in Saccharomyces carlsbergensis . Journal of Bacteriology 110:477–484
    [Google Scholar]
  33. Szekely E., Montgomery D. 1984; Glucose represses genes that encode mitochondrial components. Molecular and Cellular Biology 4:939–946
    [Google Scholar]
  34. Zimmermann F.K., Kaufmann I., Rasenberger H., Haussman P. 1977; Genetics of carbon catabolite repression in Saccharomyces cerevisiae: genes involved in the derepression process. Molecular and General Genetics 151:95–103
    [Google Scholar]
  35. Zimmermann F.K., Scheel I. 1977; Mutants of Saccharomyces cerevisiae resistant to carbon catabolite repression. Molecular and General Genetics 154:75–82
    [Google Scholar]
  36. Zimmermann F.K., Eaton N.R. 1974; Genetics of induction and catabolite repression of maltase synthesis in Saccharomyces cerevisiae . Molecular and General Genetics 134:261–272
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-5-1217
Loading
/content/journal/micro/10.1099/00221287-135-5-1217
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error