@article{mbs:/content/journal/micro/10.1099/00221287-135-5-1195, author = "Tamplin, Mark L. and Ahmed, Mohammed K. and Jalali, Reema and Colwell, Rita R.", title = "Variation in Epitopes of the B Subunit of El Tor and Classical Biotype Vibrio cholerae O1 Cholera Toxin", journal= "Microbiology", year = "1989", volume = "135", number = "5", pages = "1195-1200", doi = "https://doi.org/10.1099/00221287-135-5-1195", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-5-1195", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Variation in epitopes of the B subunit of cholera toxin (CT-B) produced by strains of El Tor and classical biotype Vibrio cholerae O1 was examined using monoclonal antibodies prepared to V. cholerae 569B CT. CT-B epitopes were markedly conserved for V. cholerae classical biotypes. In contrast, epitope variation was observed for El Tor biotypes, which produced both a classical-like CT-B and a unique CT-B lacking at least one epitope common to 569B CT-B. The missing epitope was located outside the GM1 ganglioside-binding site. From results of the study reported here, genetic divergence is exhibited in the El Tor biotype CT-B versus classical CT-B. Furthermore, at least five unique epitopes of V. cholerae 569B CT-B can be defined.", }