Polyacrylamide gel electrophoresis (PAGE) of isoenzymes of chromosomal origin, and plasmid profile analyses, were used to investigate genetic variation within and between natural populations of biovar from diverse sources. PAGE showed that, within the biovar in general, relatively few alleles existed for the enzyme loci studied. There was a strong genetic disequilibrium between loci, suggesting that chromosomal recombination is rare. Both PAGE and plasmid profile analysis revealed differences in the degree of strain polymorphism at the sites examined. At sites which were acidic, improved (by liming) and neutral, strain variation was found to be low, intermediate and high, respectively.


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