1887

Abstract

Fourteen strains of serotype were examined for their cell-surface protein antigens in terms of hydrophobicity, and immunochemical specificities. Thirteen strains were hydrophobic, while strain GS-5 was markedly hydrophilic as compared to the other strains tested. Cell-surface protein antigens were then analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis () and Western immunoblotting. A protein antigen of 190000 (PAc) was found in cell extracts and culture supernatants of all the hydrophobic strains. Neither culture supernatant nor cell extract of strain GS-5 contained PAc. Strain GS-5, however, produced extracellularly a large amount of a protein of 155000 (PAGS-5) which reacted with rabbit anti-PAc serum. Immunodiffusion analysis showed that -5 lacked a part of the antigenic moieties in the c molecule. and radioimmunoassay showed a small amount of -5 on the cell surface of strain -5. These findings suggest that -5 may correspond to c which lacks a region participating in binding of c to the streptococcal cell.

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1989-04-01
2021-10-27
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References

  1. Ayakawa G.Y., Boushell L.W., Crowley P.J., Erdos G.W., Mcarthur W.P., Bleiweis A.S. 1987; Isolation and characterization of monoclonal antibodies specific for antigen PI, a major surface protein of mutans streptococci. Infection and Immunity 55:2759–2767
    [Google Scholar]
  2. Burnette W.N. 1981; ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  3. Forester H., Hunter N., Knox K.W. 1983; Characteristics of a high molecular weight extracellular protein of Streptococcus mutans . Journal of General Microbiology 129:2779–2788
    [Google Scholar]
  4. Gibbons R.J. 1984; Adherent interactions which may affect microbial ecology in the mouth. Journal of Dental Research 63:378–385
    [Google Scholar]
  5. Hamada S., Slade H.D. 1980; Biology, immunology, and cariogenicity of Streptococcus mutans . Microbiological Reviews 44:331–384
    [Google Scholar]
  6. Hollingshead S.K., Fischetti V.A., Scott J.R. 1986; Complete nucleotide sequence of type 6 M protein of the group A Streptococcus . Journal of Biological Chemistry 261:1677–1686
    [Google Scholar]
  7. Holt R.G., Abiko Y., Saito S., Smorawinska M., Hansen J.B., Curtiss R. III 1982; Streptococcus mutans genes that code for extracellular proteins in Escherichia coli K-12. Infection and Immunity 38:147–156
    [Google Scholar]
  8. Hughes M., Machardy S.M., Sheppard A.J., Woods N.C. 1980; Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue. Infection and Immunity 27:576–588
    [Google Scholar]
  9. Kato H., Ota F., Fukui K., Yagawa K. 1986; Monoclonal antibody to Streptococcus mutans type e cell wall polysaccharide antigen. Infection and Immunity 52:628–630
    [Google Scholar]
  10. Koga T., Asakawa H., Okahashi N., Hamada S. 1986; Sucrose-dependent cell adherence and cariogenicity of serotype c Streptococcus mutans . Journal of General Microbiology 132:2873–2883
    [Google Scholar]
  11. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  12. Lehner T., Russell M.W., Caldwell J., Smith R. 1981; Immunization with purified protein antigens from Streptococcus mutans against dental caries in rhesus monkeys. Infection and Immunity 34:407–415
    [Google Scholar]
  13. Lehner T., Caldwell J., Smith R. 1985; Local passive immunization by monoclonal antibodies against streptococcal antigen I/II in the prevention of dental caries. Infection and Immunity 50:796–799
    [Google Scholar]
  14. Loesche W.J. 1986; Role of Streptococcus mutans in human dental decay. Microbiological Reviews 50:353–380
    [Google Scholar]
  15. Ma J.K.-C., Smith R., Lehner T. 1987; Use of monoclonal antibodies in local passive immunization to prevent colonization of human teeth by Streptococcus mutans . Infection and Immunity 55:1274–1278
    [Google Scholar]
  16. Mcbride B.C., Song M., Krasse B., Olsson J. 1984; Biochemical and immunological differences between hydrophobic and hydrophilic strains of Streptococcus mutans . Infection and Immunity 44:68–75
    [Google Scholar]
  17. Moro I., Russell M.W. 1983; Ultrastructural localization of protein antigens I/II and III in Streptococcus mutans . Infection and Immunity 41:410–413
    [Google Scholar]
  18. Okahashi N., Koga T., Hamada S. 1986; Purification and immunochemical properties of a protein antigen from serotype g Streptococcus mutans . Microbiology and Immunology 30:35–47
    [Google Scholar]
  19. Rundegren J., Olsson J. 1987; Interactions between salivary agglutinins and strains of Streptococcus mutans with varying degrees of surface hydrophobicity. FEMS Microbiology Letters 40:141–146
    [Google Scholar]
  20. Russell R.R.B. 1979; Wall-associated protein antigens of Streptococcus mutans . Journal of General Microbiology 114:109–115
    [Google Scholar]
  21. Russell R.R.B., Smith K. 1986; Effect of subculturing on location of Streptococcus mutans antigens. FEMS Microbiology Letters 35:319–323
    [Google Scholar]
  22. Russell M.W., Bergmeier L.A., Zanders E.D., Lehner T. 1980; Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component. Infection and Immunity 28:486–493
    [Google Scholar]
  23. Russell R.R.B., Beighton D., Cohen B. 1982; Immunisation of monkeys (Macaca fascicularis) with antigens purified from Streptococcus mutans . British Dental Journal 152:81–84
    [Google Scholar]
  24. Scully C.M., Russell M.W., Lehner T. 1980; Specificity of opsonizing antibodies to antigens of Streptococcus mutans . Immunology 41:467–473
    [Google Scholar]
  25. Westergren G., Olsson J. 1983; Hydrophobicity and adherence of oral streptococci after repeated subculture in vitro. Infection and Immunity 40:432–435
    [Google Scholar]
  26. Zanders E.D., Lehner T. 1981; Separation and characterization of a protein antigen from cells of Streptococcus mutans . Journal of General Microbiology 122:217–225
    [Google Scholar]
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