1887

Abstract

Photosensitizing activity of haematoporphyrin (Hp) on cells is mainly promoted by unbound dye molecules in the bulk aqueous medium. Moreover, the death of photosensitized cells is dependent on the dye concentration, irradiation time, irradiation temperature, and the composition of the growth media. Morphological and biochemical studies indicate that the photoprocess involves an initial limited alteration of the cytoplasmic membrane, which allows the penetration of the dye into the cell with consequent photodamage of intracellular targets. In this respect, the Hp-sensitized photoinactivation of eukaryotic microbial cells differs from that in prokaryotic cells.

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/content/journal/micro/10.1099/00221287-135-4-957
1989-04-01
2022-01-19
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References

  1. Azumi I., McGlynn S.P. 1962; Polarization of the luminescence of phenanthrene. Journal of Chemical Physics 37:2413–2420
    [Google Scholar]
  2. Bertoloni G., Salvato B., Dall’Acqua M., Vaz-Zoler M., Jori G. 1984; Hematoporphyrin-sensitized photoinactivation ofStreptococcus faeca-lis . Photochemistry and Photobiology 39:811–816
    [Google Scholar]
  3. Bertoloni G., Viel A., Grossato A., Jori G. 1985; The photosensitizing activity of haematopor-phyrin on Mollicutes. Journal of General Microbiology 131:2217–2223
    [Google Scholar]
  4. Bertoloni G., Zambotto F., Conventi L., Reddi E., Jori G. 1987; Role of specific cellular targets in the hematoporphyrin-sensitized photoinactivation of microbial cells. Photochemistry and Photobiology 46:695–698
    [Google Scholar]
  5. Gläser H.U., Höfer M. 1986; Effect of cations on the plasma-membrane-bound ATPase from the yeast Metschnikowia reukaufi . Journal of General Microbiology 132:2615–2620
    [Google Scholar]
  6. Ito T. 1981; Photodynamic action of hematopor-phyrin on yeast cells - a kinetic approach. Photochemistry and Photobiology 34:521–524
    [Google Scholar]
  7. Ito T. 1983; Photodynamic agents as tools for cell biology. Photochemical and Photobiological Reviews 7:141–186
    [Google Scholar]
  8. Jori G., Reddi E., Cozzani I., Tomio L. 1986; Controlled targeting of subcellular sites by porphyrins in tumour-bearing mice. British Journal of Cancer 53:615–621
    [Google Scholar]
  9. Marriot M.S. 1975; Enzymic activity of purified plasma membranes from the yeast and mycelial forms of Candida albicans . Journal of General Microbiology 89:345–352
    [Google Scholar]
  10. Moan J., Stenstrom A.G.K. 1981; Effect of phosphate and chloride on the photodynamic inactivation of yeast cells. Photochemistry and Photobiology 33:761–763
    [Google Scholar]
  11. Moan J., Sommer S. 1985; Oxygen-dependence of the photosensitizing effect of hematoporphyrin derivative in NHIK 3025 cells. Cancer Research 45:1608–1610
    [Google Scholar]
  12. Poulter R., Jeffery K., Hubbard M.J., Shepherd M.G., Sullivan P.A. 1981; Parasexual genetic analysis of Candida albicans by spheroplast fusion. Journal of Bacteriology 146:833–840
    [Google Scholar]
  13. Reynolds E.W. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  14. Spikes J.D. 1988; Photosensitization. In The Science of Photobiology, 2nd edn.. Smith K. C. Edited by New York: Plenum Publishing Corporation;
    [Google Scholar]
  15. Srivastava R.C., Anand V.D., Carper W.R. 1973; A fluorescence study of hematoporphyrin. Applied Spectroscopy 27:444–449
    [Google Scholar]
  16. Sud I.J., Feinegold D.S. 1981; Mechanisms of action of the antimycotic imidazoles. Journal of Investigative Dermatology 76:438–441
    [Google Scholar]
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