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Volume 135, Issue 4

Physical and Genetic Characterization of Chromosomal Copies of the Free

Abstract

The two linear, integrated mini-circle copies of A3(2) were cloned in and their positions on the genetic map were determined. Mini-circle copy A is close to the , 20 kb upstream of the operon. Mini-circle copy B is close to the locus and is absent from J1501, which has suffered a chromosomal deletion including mini-circle copy B and possibly associated with the mutation. The mini-circle copies were not involved in any of the several previously identified physical interactions between the plasmid SCP1 and the chromosome. A new insertion sequence was identified close to the right end of mini-circle copy B in M145. The free mini-circle of , when inserted into an -deleted derivative of phage C31, actively integrated this phage into the chromosomes of and at preferred and secondary sites. The resulting prophages were stably inherited and remained physically intact. No precise excision of prophages from lysogens carrying insertions at preferred or secondary integration sites was detected: instead, free phages were generated by imprecise excisions. These phages allowed the cloning of segments (> 3 kb in length) of the chromosomal DNA flanking preferred and secondary integration sites. Attempts to delete the preferred integration site by double homologous recombination with a clone carrying flanking DNA sequences and an antibiotic resistance gene were unsuccessful.

  • Received:
  • Accepted:
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  • Published Online:
© Society for General Microbiology, 1989
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1989-04-01
2024-04-25
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References

  1. Anzai H., Kumada Y., Hara O., Murakami T., Itoh R., Tamkano E., Imia S., Satoh A., Nagaoka K. 1988; Replacement of Streptomyces hygroscopicus genomic segments with in vitro altered DNA sequences. Journal of Antibiotics 41:226–233
     [Google Scholar]
  2. Bibb M.J., Freeman R.F., Hopwood D.A. 1977; Physical and genetic characterisation of a second sex factor, SCP2, for S. coelicolor A3(2). Molecular and General Genetics 154:155–166
     [Google Scholar]
  3. Bibb M.J., Ward J.M., Kieser T., Cohen S.N., Hopwood D.A. 1981; Excision of chromosomal DNA sequences from S. coelicolor forms a novel family of plasmids detectable in S. lividans . Molecular and General Genetics 184:230–240
     [Google Scholar]
  4. Bibb M.J., Bibb M.J., Ward J.M., Cohen S.N. 1985; Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces . Molecular and General Genetics 199:26–36
     [Google Scholar]
  5. Caillaud F., Courvalin P. 1987; Nucleotide sequence of the ends of the conjugative shuttle transposon Tn]545 . Molecular and General Genetics 209:110–115
     [Google Scholar]
  6. Calos M.P., Miller J.H. 1980; Transposable elements. Cell 20:579–595
     [Google Scholar]
  7. Chater K.F., Bruton C.J., King A.A., Suarez J.E. 1982; The expression of Streptomyces and E.coli drug-resistance determinants cloned in the Streptomyces phage 𝜙31. Gene 19:21–32
     [Google Scholar]
  8. Chater K.F., Bruton C.J., Foster S.G., Tobek I. 1985; Physical and genetic analysis of IS 110, a transposable element of S. coelicolor A3(2). Molecular and General Genetics 200:235–239
     [Google Scholar]
  9. Chinenova T.A., Mkrtumian N.M., Lomov-Skaya N.D. 1982; Genetic characterisation of a new character of phage resistance in S. coelicolor A3(2). Genetika 18:1945–1952
     [Google Scholar]
  10. Chung S.-T. 1987; Tn4556, a 6·8 kb transposable element of S. fradiae . Journal of Bacteriology 169:4436–4441
     [Google Scholar]
  11. Deonier R.C., Hadley R.G. 1980; IS2-IS2 and IS3-IS3 relative recombination frequencies in F integration. Plasmid 3:48–64
     [Google Scholar]
  12. Eckhardt T., Smith R. 1987; Use of a secreted β- galactosidase to study gene expression and secretion in Streptomyces . In Genetics of Industrial Microorganisms, Part B pp. 17–27 Alacevic M., Hranueli D., Toman Z. Edited by Zagreb: Pliva;
     [Google Scholar]
  13. Fisher S.H., Bruton C.J., Chater K.F. 1987; The glucose kinase gene of S. coelicolor and its use in selecting spontaneous deletions for desired regions of the genome. Molecular and General Genetics 20 6:35–44
     [Google Scholar]
  14. Grosveld F.G., Lund T., Murray E.J., Mellor A.L., Dahl H.M., Flavell R.A. 1982; The construction of cosmid libraries which can be used to transform eukaryotic cells. Nucleic Acids Research 10:6715–6732
     [Google Scholar]
  15. Guyer M.S., Reed R.R., Steitz J.A., Low K.B. 1980; Identification of a sex-factor-affinity site in E. coli as yS . Cold Spring Harbor Symposia on Quantitative Biology 45:135–140
     [Google Scholar]
  16. Hodgson D.A. 1982; Glucose repression of carbon source uptake and metabolism in Streptomyces coelicolor A3(2) and its perturbation in mutants resistant to 2-deoxyglucose. Journal of General Microbiology 128:2417–2430
     [Google Scholar]
  17. Hopwood D.A., Wright H.M. 1976a; Genetic studies of SCP1-prime strains of Streptomyces coelicolor A3(2). Journal of General Microbiology 95:107–120
     [Google Scholar]
  18. Hopwood D.A., Wright H.M. 1976b; Interactions of the plasmid SCP1 with the chromosome of S. coelicolor A3(2). In The Second International Symposium on the Genetics of Industrial Microorganisms pp. 607–619 Macdonald K.D. Edited by London: Academic Press;
     [Google Scholar]
  19. Hopwood D.A., Kieser T., Wright H.M., Bibb M.J. 1983; Plasmids, recombination and chromosome mapping in Streptomyces lividans 66 . Journal of General Microbiology 129:2257–2269
     [Google Scholar]
  20. Hopwood D.A., Lydiate D.J., Malpartida F., Wright H.M. 1984; Conjugative sex plasmids of Streptomyces . In Plasmids in Bacteria pp. 615–634 Helinski D., Cohen S., Clewell D. Edited by New York: Plenum;
     [Google Scholar]
  21. Hopwood D.A., Bibb M.J., Chater K.F., Kieser T., Bruton C.J., Kieser H.M., Lydiate D.J., Smith C.P., Ward J.M., Schrempf H. 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich: John Innes Foundation;
     [Google Scholar]
  22. Ikeda H., Seno E.T., Bruton C.J., Chater K.F. 1984; Genetic mapping, cloning and physiological aspects of the glucose kinase gene of S. coelicolor . Molecular and General Genetics 196:501–507
     [Google Scholar]
  23. Katz E., Thompson C.J., Hopwood D.A. 1982; Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans . Journal of General Microbiology 129:2703–2714
     [Google Scholar]
  24. Kendall K., Cullum J. 1986; Identification of a DNA sequence associated with plasmid integration in S. coelicolor A3(2). Molecular and General Genetics 202:240–245
     [Google Scholar]
  25. Lomovskaya N.D., Mkrtumian N.M., Gostim-Skaya N.L., Danilenko V.N. 1972; Characterization of temperate actinophage 𝜙C3l isolated from S. coelicolor A3(2). Journal of Virology 9:258–262
     [Google Scholar]
  26. Lydiate D.J., Ikeda H., Hopwood D.A. 1986; A 2·6 kb DNA sequence of S. coelicolor A3(2) which functions as a transposable element. Molecular and General Genetics 203:79–88
     [Google Scholar]
  27. Lydiate D.J., Henderson D.J., Ashby A.M., Hopwood D.A. 1987; Transposable elements of Streptomyces coelicolor A3(2). In Genetics of Industrial Micro-organisms, Part B pp. 49–56 Alacevic M., Hranueli D., Toman Z. Edited by Zagreb: Pliva;
     [Google Scholar]
  28. Lydiate D.J., Mendez C., Kieser H.M., Hopwood D.A. 1988; Mutation and cloning of clustered Streptomyces genes essential for sulphate metabolism. Molecular and General Genetics 211:415–423
     [Google Scholar]
  29. Madon J., Moretti P., Hütter R. 1987; Site- specific integration and excision of pMEAlOO in Nocardia mediterranei . Molecular and General Genetics 209:257–264
     [Google Scholar]
  30. Malpartida F., Hallam S.E., Kieser H.M., Motamedi H., Hutchinson CR., Butler M.J., Sugden D.A., Warren M., Mckillop C., Bailey C.R., Humphreys G.O., Hopwood D.A. 1987; Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthesis genes. Nature; London: 325818–821
     [Google Scholar]
  31. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
     [Google Scholar]
  32. Murray N.E., Brammar W.J., Murray K. 1977; Lambdoid phages that simplify the recovery of in vitro recombinants. Molecular and General Genetics 150:53–61
     [Google Scholar]
  33. Rodicio M.R., Bruton C.J., Chater K.F. 1985; New derivatives of the it Streptomyces temperate phage 𝜙C31 useful for the cloning and functional analysis of Streptomyces DNA. Gene 34:283–292
     [Google Scholar]
  34. Rüther U., Koenen M., Otto K., Muller-Hill B. 1981; pUR222, a vector for cloning and rapid chemical sequencing of DNA. Nucleic Acids Research 9:4087–4098
     [Google Scholar]
  35. Seno E.T., Bruton C.J., Chater K.F. 1984; The glycerol utilization operon of 5. oelicolor: genetic mapping of gyl mutations and the analysis of cloned gyl DNA. Molecular and General Genetics 193:119–128
     [Google Scholar]
  36. Smith C.P., Chater K.F. 1988; Cloning and transcription analysis of the entire glycerol utilisation (gylABX) operon of S. coelicolor A3(2) and identification of a closely associated transcription unit. Molecular and General Genetics 211:129–137
     [Google Scholar]
  37. Soberon X., Covarrubias L., Bolivar F. 1980; Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene 9:287–305
     [Google Scholar]
  38. Vivian A., Hopwood D.A. 1970; Genetic control of fertility in Streptomyces coelicolor A3(2): the IF fertility type. Journal of General Microbiology 64:101–117
     [Google Scholar]
  39. Vivian A., Hopwood D.A. 1973; Genetic control of fertility in Streptomyces coelicolor A3(2): new kinds of donor strains. Journal of General Microbiology 76:147–162
     [Google Scholar]
  40. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved Ml3 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
     [Google Scholar]
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Physical and Genetic Characterization of Chromosomal Copies of the Streptomyces coelicolor Mini-circle
Microbiology 135, 941 (1989); https://doi.org/10.1099/00221287-135-4-941
/content/journal/micro/10.1099/00221287-135-4-941
/content/journal/micro/10.1099/00221287-135-4-941
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