RT Journal Article SR Electronic(1) A1 Baird, Paul N. A1 Hall, Lucinda M. C. A1 Coates, Anthony R. M.YR 1989 T1 Cloning and Sequence Analysis of the 10 kDa Antigen Gene of Mycobacterium tuberculosis JF Microbiology, VO 135 IS 4 SP 931 OP 939 DO https://doi.org/10.1099/00221287-135-4-931 PB Microbiology Society, SN 1465-2080, AB The gene encoding a major protein antigen of Mycobacterium tuberculosis has been cloned and sequenced using oligonucleotide probes derived from the N-terminal sequence of the analogous protein from Mycobacterium bovis BCG. The gene analysis revealed a sequence encoding a protein of 99 amino acid residues, with a molecular mass of 10ยท7 kDa. Computer prediction suggests that the protein contains three T-cell-determined epitopes (of which one has been demonstrated experimentally) and three B-cell-determined epitopes. The protein sequence was homologous to two prokaryote heat-shock proteins and the gene possessed heat-shock-like promoter sequences upstream of the initiation codon. A hairpin loop identified in the upstream sequence may also be important in regulation of the gene., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-4-931