1887

Abstract

Endo-1, 4--glucanase genes have been cloned from two strains of recently isolated in this laboratory. Although the strains were phenotypically similar, cross-hybridization studies between them showed significant genetic differences, with only 20% of the genome forming DNA heteroduplexes. Heteroduplexes displayed an average dissociation temperature 9 °C lower than that of the homoduplex. Consistent with this, restriction maps of the two endoglucanase genes showed no similarity, and hybridization work using the endoglucanase genes as probes revealed that neither gene was present in the genome of the other isolate of . Comparative enzyme characterization showed differences between the enzymes in their response to temperature, pH and substrate preference.

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1989-04-01
2021-05-17
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References

  1. Ashwell G. 1957; Colorimetric analysis of sugars. Methods in Enzymology 3:73–105
    [Google Scholar]
  2. Barros M.E.C., Thomson J.A. 1987; Cloning and expression in E. coli of a cellulase gene from Ruminococcus flavefaciens . Journal of Bacteriology 169:1760–1762
    [Google Scholar]
  3. Dellaglio F., Torriani S. 1986; DNA-DNA homology, physiological characteristics and distribution of lactic acid bacteria isolated from maize silage. Journal of Applied Bacteriology 60:83–92
    [Google Scholar]
  4. De Ley J., Tijtgat R., De Smedt J., Michiels M. 1983; Thermal stability of DNA:DNA hybrids within the genus Agrobacterium . Journal of General Microbiology 78:241–252
    [Google Scholar]
  5. Hanahan D. 1985; Techniques for transformation of E. coli . In DNA Cloning: a Practical Approach 1 Glover D. M. Edited by Oxford: IRL Press;
    [Google Scholar]
  6. Hood D.W., Dow C.S., Green P.N. 1987; DNA:DNA hybridization studies on the pink-pigmented facultative methylotrophs. Journal of General Microbiology 133:709–720
    [Google Scholar]
  7. Hungate R.E. 1966 The Rumen and its Microbes. New York:: Academic Press.;
    [Google Scholar]
  8. Hungate R.E. 1969; A roll tube method for cultivation of strict anaerobes. Methods in Microbiology 3B:117–132
    [Google Scholar]
  9. Kawai S., Honda H., Tanase T., Taya M., Iijima S., Kobayashi T. 1987; Molecular cloning of Ruminococcus albuscellulase gene. Agricultural and Biological Chemistry 51:59–63
    [Google Scholar]
  10. Kim J., Kong I., Yu J. 1987; Molecular cloning of an endoglucanase gene from an alkalophilicBacillus sp. and its expression in E. coli . Applied and Environmental Microbiology 53:2656–2659
    [Google Scholar]
  11. Leder P., Tiemeier D., Enquist L. 1977; EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: The λgtWES system. Science 196:175–177
    [Google Scholar]
  12. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  13. Mannarelli B.M., Lee D. 1987; Differentiation of Butyrivibrio fibrisolvens strains into several species by G + C content and DNA:DNA hybridization data. Abstracts of Conference on Rumen Function; Chicago, Illinois 19: p. 35:
    [Google Scholar]
  14. Morris E.J., Cole O.J. 1987; Relationship between cellulolytic activity and adhesion to cellulose in Ruminococcus albus . Journal of General Microbiology 133:1023–1032
    [Google Scholar]
  15. Murray N.E., Brammar W.J., Murray K. 1977; Lamboid phages that simplify the recovery of in vitro recombinants. Molecular and General Genetics 150:53–61
    [Google Scholar]
  16. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153:375–380
    [Google Scholar]
  17. Pettipher G.L., Latham M.J. 1979; Characteristics of enzymes produced by Ruminococcus flavefaciens which degrade plant cell walls. Journal of General Microbiology 110:21–27
    [Google Scholar]
  18. Playne M.J. 1985; Determination of ethanol, volatile fatty acids, lactic and succinic acids in fermentation liquids by gas chromatography. Journal of the Science of Food and Agriculture 36:638–644
    [Google Scholar]
  19. Roberts M.C., Moncla B., Kenny G.E. 1987; Chromosomal DNA probes for the identification of Bacteroides species. Journal of General Microbiology 133:1423–1430
    [Google Scholar]
  20. Salyers A.A., Lynn S.P., Gardner J.F. 1983; Use of randomly cloned DNA fragments for identification of Bacteroides thetaiotaomicron . Journal of Bacteriology 154:287–293
    [Google Scholar]
  21. Smith C.J., Hespell R.B. 1983; Symposium: Application of Molecular Genetics in Ruminants. Journal of Dairy Science 66:1536–1546
    [Google Scholar]
  22. Southern E. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  23. Taylor K.A., Crosby B., Mcgavin M., Forsberg C.W., Thomas D.Y. 1987; Characteristics of an endoglucanase encoded by a cel gene from Bacteroides succinogenes expressed in E. coli . Applied and Environmental Microbiology 53:41–46
    [Google Scholar]
  24. Teather R.M., Wood P.J. 1982; Use of congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Applied and Environmental Microbiology 43:777–780
    [Google Scholar]
  25. Wahl G.M., Stern M., Stark G.R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethal paper and rapid hybridization by using dextran sulfate. Proceedings of the National Academy of Sciences of the United States of America 76:3683–3687
    [Google Scholar]
  26. Williams A.G., Withers S.E. 1982; The production of plant cell wall polysaccharide-degrading enzymes by hemicellulolytic rumen bacterial isolates grown on a range of carbohydrate substrates. Journal of Applied Bacteriology 52:377–387
    [Google Scholar]
  27. Wood T.M. 1971; The cellulase of Fusarium solani . Biochemical Journal 121:353–362
    [Google Scholar]
  28. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved Ml3 phage cloning vectors and host strains: nucleotide sequences of the MBmp 18 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  29. Zappe H., Jones D.T., Woods D.R. 1986; Cloning and expression of Clostridium acetobutylicum endoglucanase, cellobiase and amino acid biosynthesis genes in Escherichia coli . Journal of General Microbiology 132:1367–1372
    [Google Scholar]
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