1887

Abstract

Two new temperate bacteriophages for , designated RsA and RsD, were isolated from a stock of phage RsGl using R. strain DSM 159–2 as the indicator strain. Electron-microscopic examination showed that phage RsA belonged to group B and phage RsD belonged to group A of Bradley’s morphological classification. Phage RsA had a polyhedral head (50 nm in diameter) and a long, flexible, non-contractile tail (250 by 11 nm). Phage RsD also had a polyhedral head (56 nm in diameter) and a sheathed contractile tail (160 by 18 nm). Both phages contained double-stranded DNA with cohesive ends. The size of the RsA genome was about 40 kb and the G + C content was 72·4 mol%. The size of the RsD genome was about 56 kb and the G + C content was approximately 72·6 mol%. Biotinylated DNA probes of phage RsA and of phage RsD hybridized to genomic DNA from strains Y and Si 4 but not to DNA from other sources. The host ranges of the two phages were determined using 26 strains and six strains of . Phage RsA formed plaques on DSM 159–2 only, while phage RsD plaqued on another six strains of but not on .

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1989-04-01
2021-05-14
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