Transfer and Expression of an Cellulase Gene in Free

Abstract

The cellulase gene from coding for endoglucanase Z was subcloned into a broad-host-range plasmid pGSS33 in The recombinant pNB20 was transferred into ATCC 10988 by mobilization using the helper plasmid RP4. Plasmid pNB20 was stably maintained in and hosts. The endoglucanase gene was expressed efficiently and the level of expression was higher in than in The specific activity of the enzyme was comparable to that of the parent strain of The proteins produced by and presented identical immunological and electrophoretic properties. Biosynthesis of endoglucanase occurred during the exponential growth phase of and about 35% of the enzyme was released into the medium in the absence of detectable cell lysis. The endoglucanase appeared to be located in the periplasmic space in

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-4-893
1989-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/4/mic-135-4-893.html?itemId=/content/journal/micro/10.1099/00221287-135-4-893&mimeType=html&fmt=ahah

References

  1. Afendra A.S., Drainas C. 1987; Expression and stability of a recombinant plasmid in Zymomonas mobilis and Escherichia coli . Journal of General Microbiology 133:127–134
    [Google Scholar]
  2. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Boyer H.W., Rouland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli . Journal of Molecular Biology 41:459–472
    [Google Scholar]
  4. Boyer M.H., Chambost J.P., Magnan M., Cattaneo J. 1984a; Carboxymethyl-cellulase from Erwinia chrysanthemi. I. Production and regulation of extracellular carboxymethyl-cellulase. Journal of Biotechnology 1:229–239
    [Google Scholar]
  5. Boyer M.H., Chambost J.P., Magnan M., Cattaneo J. 1984b; Carboxymethyl-cellulase from Erwinia chrysanthemi. II. Purification and partial characterization of an endo-β,4-glucanase. Journal of Biotechnology 1:241–252
    [Google Scholar]
  6. Boyer M.H., Cami B., Kotoujanski A., Chambost J.P., Frixon C. 1987; Isolation of the gene encoding the major endoglucanase of Erwinia chrysanthemi. Homology between cel genes of two strains of Erwinia chrysanthemi . FEMS Microbiology Letters 41:351–356
    [Google Scholar]
  7. Brestic-Goachet N., Gunasekaran P., Cami B., Baratti J. 1987; Transfer and expression of broad host range plasmids in Zymomonas mobilis . Biotechnology Letters 9:13–18
    [Google Scholar]
  8. Byun M.O.K., Kaper J.B., Ingram L.O. 1986; Construction of a new vector for the expression of foreign genes in Zymomonas mobilis . Journal of Industrial Microbiology 1:9–15
    [Google Scholar]
  9. Carey V.C., Walia S.K., Ingram L.O. 1983; Expression of a lactose transposon (Tn957) in Zymomonas mobilis . Applied and Environmental Microbiology 46:1163–1168
    [Google Scholar]
  10. Conway T., Byun M.O.K., Ingram L.O. 1987 a; Expression vector for Zymomonas mobilis . Applied and Environmental Microbiology 53:235–241
    [Google Scholar]
  11. Conway T., Osman Y.A., Konnan J.I., Hoffman E.M., Ingram L.O. 1987b; Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. Journal of Bacteriology 169:949–954
    [Google Scholar]
  12. Conway T., Osman Y.A., Ingram L.O. 1987C; Gene expression in Zymomonas mobilis: promoter structure and identification of membrane anchor sequences forming functional LacZ’ fusion proteins. Journal of Bacteriology 169:2327–2335
    [Google Scholar]
  13. Datta N., Hedges R.W., Shaw E.J., Sykes R.B., Richmond M.H. 1971; Properties of an R factor from Pseudomonas aeruginosa . Journal of Bacteriology 108:1244–1249
    [Google Scholar]
  14. Diano M., Lebivic A., Hirn M. 1987; A method for the production of highly specific polyclonal antibodies. Analytical Biochemistry 166:224–229
    [Google Scholar]
  15. Hawley D.K., Mcclure W.R. 1983; Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Research 11:2237–2255
    [Google Scholar]
  16. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  17. Lejeune A., Eveleigh D., Colson C. 1988; Expression of an endoglucanase gene of Pseudomonas fluorescens var.ellulosa in Zymomonas mobilis . FEMS Microbiology Letters 49:363–366
    [Google Scholar]
  18. Luria S.F., Delbruck M. 1943; Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28:491–511
    [Google Scholar]
  19. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Miller J.H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Neu H.C., Heppel L.A. 1965; The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. Journal of Biological Chemistry 240:3685–3692
    [Google Scholar]
  22. Norrander J., Kempe T., Messing J. 1983; Construction of improved Ml3 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26:101–106
    [Google Scholar]
  23. Nossal N.G., Heppel L.A. 1966; The release of enzymes by osmotic shock from Escherichia coli in exponential phase. Journal of Biological Chemistry 241:3055–3062
    [Google Scholar]
  24. Ouchterlony O. 1958; Diffusion in gel methods for immunological analysis. Progress in Allergy 5:1–9
    [Google Scholar]
  25. Park J.T., Johnson M.S. 1949; A submicrodetermination of glucose. Journal of Biological Chemistry 181:149–151
    [Google Scholar]
  26. Rogers P.L., Lee K.L., Skotnicki M.L., Tribe D.E. 1982; Ethanol production by Zymomonas mobilis . Advances in Biochemical Engineering 23:37–84
    [Google Scholar]
  27. Sharpe G.S. 1984; Broad host range cloning vectors for Gram-negative bacteria. Gene 29:93–102
    [Google Scholar]
  28. Skotnicki M.L., Lee K.J., Tribe D.E., Rogers P.L. 1981; Comparison of ethanol production by different Zymomonas strains. Applied and Environmental Microbiology 41:889–893
    [Google Scholar]
  29. Teather R.M., Wood P.J. 1982; Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Applied and Environmental Microbiology 43:777–780
    [Google Scholar]
  30. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  31. Ulrich R. 1982; pUR250 allows rapid chemical sequencing of both DNA strands of its inserts. Nucleic Acids Research 10:5765–5772
    [Google Scholar]
  32. Willis R.C., Cirakoglu R.G., Morris C., Schellenberg G.D., Gerber N.H., Furiong C.E. 1974; Preparation of the periplasmic binding proteins from Salmonella typhimurium and Escherichia coli . Archives of Biochemistry and Biophysics 161:64–75
    [Google Scholar]
  33. Yanase H., Yasui M., Miyazaki T., Tonomura K. 1985; Fusion of spheroplasts and genetic recombination of Zymomonas mobilis . Agricultural and Biological Chemistry 49:133–140
    [Google Scholar]
  34. Yanase H., Kurii J., Tonomura K. 1986; Construction of a promoter-cloning vector in Zymomonas mobilis . Agricultural and Biological Chemistry 50:2959–2961
    [Google Scholar]
  35. Yoon K.H., Park S.H., Pack M.Y. 1988; Transfer of Bacillus subtilis endo-β-1,4-glucanase gene into Zymomonas anaerobia . Biotechnology Letters 10:213–216
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-4-893
Loading
/content/journal/micro/10.1099/00221287-135-4-893
Loading

Data & Media loading...

Most cited Most Cited RSS feed