@article{mbs:/content/journal/micro/10.1099/00221287-135-4-893, author = "Brestic-Goachet, Nadine and Gunasekaran, Paramasamy and Cami, Brigitte and Baratti, Jacques C.", title = "Transfer and Expression of an Erwinia chrysanthemi Cellulase Gene in Zymomonas mobilis", journal= "Microbiology", year = "1989", volume = "135", number = "4", pages = "893-902", doi = "https://doi.org/10.1099/00221287-135-4-893", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-4-893", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "The cellulase gene from Erwinia chrysanthemi coding for endoglucanase Z was subcloned into a broad-host-range plasmid pGSS33 in Escherichia coli. The recombinant pNB20 was transferred into Zymomonas mobilis ATCC 10988 by mobilization using the helper plasmid RP4. Plasmid pNB20 was stably maintained in E. coli and Z. mobilis hosts. The endoglucanase gene celZ was expressed efficiently and the level of expression was higher in Z. mobilis than in E. coli. The specific activity of the enzyme was comparable to that of the parent strain of Er. chrysanthemi. The proteins produced by Z. mobilis and Er. chrysanthemi presented identical immunological and electrophoretic properties. Biosynthesis of endoglucanase occurred during the exponential growth phase of Z. mobilis and about 35% of the enzyme was released into the medium in the absence of detectable cell lysis. The endoglucanase appeared to be located in the periplasmic space in Z. mobilis. ", }