@article{mbs:/content/journal/micro/10.1099/00221287-135-4-873, author = "Kuroda, Takashi and Morita, Masashi and Okai, Kazuhisa and Okamura, Shoji and Nishi, Arasuke", title = "Isolation of Plasma Membrane and Analysis of Membrane Glycoproteins from the Slime Mould Physarum polycephalum", journal= "Microbiology", year = "1989", volume = "135", number = "4", pages = "873-881", doi = "https://doi.org/10.1099/00221287-135-4-873", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-4-873", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Plasma membranes were isolated from the acellular slime mould Physarum polycephalum by differential centrifugation and an aqueous two-phase polymer method. ATPase activity in the membrane fraction was optimal at pH 6ยท5 and was severely inhibited by vanadate but resistant to oligomycin. The protein components of the plasma membrane were analysed by polyacrylamide gel electrophoresis. Glycoproteins were located on Western blots by incubation of the sheets with lectin-peroxidase reagents. The results indicated that most of the membrane proteins were glycosylated. Pulse-chase experiments with [3H]glucosamine showed that when the plasmodia were induced to differentiate to macrocysts, turnover of the membrane glycoproteins occurred more rapidly than in growing plasmodia.", }