1887

Abstract

The differentiation of proheterocysts and heterocysts in was initiated by the removal of NHCl from the medium. The development of both proheterocysts and mature heterocysts was inhibited by rifampicin, chloramphenicol and mitomycin C, at final concentrations of 0.2, 4.0 and 1.0 μg ml, respectively. However, lower concentrations than these were sufficient to inhibit mature heterocyst development while permitting proheterocyst formation. The presence of rifampicin (0.2 μg ml) during the early stages of the induction of differentiation, just prior to the initial visual detection of proheterocysts, led to an increase in the frequency of heterocysts that subsequently developed. To assess the commitment of cells to differentiation, samples of culture were incubated with inhibitors or NHCl, or were transferred to darkness, at different times following the initiation of development. Each treatment produced a characteristic commitment time which differed for proheterocysts and mature heterocysts. The data obtained from these experiments have been used to formulate a model for the control of heterocyst development in in which DNA replication serves as a timer mechanism for the process.

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1989-04-01
2024-04-25
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References

  1. Adams D.G. 1978 Differentiation, pattern control and macromolecule synthesis in the blue-green alga, Anabaena cylindrica PhD thesis Liverpool University.:
    [Google Scholar]
  2. Adams D.G., Carr N.G. 1979; The control of heterocyst development and spacing in A. cylindrica . In Abstracts of the Third International Symposium on Photosynthetic Prokaryotes, Oxford D30 Nichols J. M. Edited by Liverpool: Liverpool University;
    [Google Scholar]
  3. Adams D.G., Carr N.G. 1981; The developmental biology of heterocyst and akinete formation in cyanobacteria. CRC Critical Reviews in Microbiology 9:45–100
    [Google Scholar]
  4. Allen M.B., Arnon D.I. 1955; Studies on nitrogen-fixing blue-green algae. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiology 30:366–372
    [Google Scholar]
  5. Bradley S., Carr N.G. 1976; Heterocyst and nitrogenase development in Anabaena cylindrica . Journal of General Microbiology 96:175–184
    [Google Scholar]
  6. Bradley S., Carr N.G. 1977; Heterocyst development in Anabaena cylindrica: the necessity for light as an initial trigger and sequential stages of commitment. Journal of General Microbiology 101:291–297
    [Google Scholar]
  7. Doolittle W.F. 1979; The cyanobacterial genome, its expression and the control of that expression. Advances in Microbial Physiology 20:1–102
    [Google Scholar]
  8. Fleming H., Haselkorn R. 1974; The program of protein synthesis during heterocyst differentiation in nitrogen-fixing blue-green algae. Cell 3:159–170
    [Google Scholar]
  9. Hartmann G., Honikel K.O., Knusel F., Nuesch J. 1967; The specific inhibition of the DNA-directed RNA synthesis by rifamycin. Biochimica et biophysica acta 145:843–844
    [Google Scholar]
  10. Kulasooriya S.A., Fay P. 1971; Heterocyst differentiation and nitrogen fixation in Anabaena cylindrica . British Phycological Journal 6:270
    [Google Scholar]
  11. Neuhoff V., Schill W.-B., Sternbach H. 1970; Micro-analysis of pure deoxyribonucleic acid-dependent ribonucleic acid polymerase from Escherichia coli. Action of heparin and rifampicin on structure and function. Biochemical Journal 117:623–631
    [Google Scholar]
  12. Osley M.A., Newton A. 1980; Temporal control of the cell cycle in Caulobacter crescentus: roles of DNA chain elongation and completion. Journal of Molecular Biology 138:109–128
    [Google Scholar]
  13. Wilcox M., Mitchison G.J., Smith R.J. 1973; Pattern formation in the blue-green alga, Anabaena. II. Controlled proheterocyst regression. Journal of Cell Science 13:637–649
    [Google Scholar]
  14. Wilcox M., Mitchison G.J., Smith R.J. 1975; Spatial control of differentiation in the blue-green alga Anabaena . In Microbiology 1975 pp. 453–463 Schlessinger D. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Wolk C.P. 1975; Differentiation and pattern formation in filamentous blue-green algae. In Spores VI pp. 85–96 Gerhardt P., Sadoff H., Costilow R. Edited by Washington DC.: American Society for Microbiology.;
    [Google Scholar]
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