1887

Abstract

Fatty acid analysis was done with GC and GC-MS on 21 strains of ‘’ representative of the various proposed species. Although no qualitative differences were found in the fatty acid profiles, discriminant analysis of the quantitative data revealed three groups. and were indistinguishable but separated from the other two groups which comprised with a wide fermentation pattern and with a narrow fermentation pattern. Three of the strains could be distinguished from the others by a ‘fingerprint’ of a particularly prominent fatty acid peak. The results support the suggestion that there is more than one species in this group of organisms and that the technique might be of value in epidemiological investigations of ‘

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-4-831
1989-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/4/mic-135-4-831.html?itemId=/content/journal/micro/10.1099/00221287-135-4-831&mimeType=html&fmt=ahah

References

  1. Ball L.C., Parker M.T. 1979; The cultural and biochemical characters of Streptococcus milleri strains isolated from human sources. Journal of Hygiene 82:63–78
    [Google Scholar]
  2. Brian B.L., Gardener E.L. 1968; A simple procedure for detecting the presence of cyclopropane fatty acids in bacterial lipids. Applied Microbiology 16:549–552
    [Google Scholar]
  3. Colman G., Williams R.E.O. 1972; Taxonomy of some human viridans streptococci. In Streptococci and Streptococcal Diseases pp. 281–299 Wannamaker L. W., Matsen J. M. Edited by New York: Academic Press;
    [Google Scholar]
  4. Coykendall A.L., Wesbecher P.M., Gustafson K.B. 1987; Genetic similarities among four species of Streptococcus. S. milleri, S. anginosus, S. constellatus and S. intermedius . International Journal of Systematic Bacteriology 37:222–228
    [Google Scholar]
  5. Dawidowicz E.R., Thompson T.E. 1971; Artifacts produced by boron trifluoride methanolysis of a synthetic lecithin containing cyclopropane fatty acids. Journal of Lipid Research 12:636–637
    [Google Scholar]
  6. Dixon W.J. 1983 In BMDP Statistical Software pp. 519–537 Dixon W. J. Edited by Berkeley: University of California Press;
    [Google Scholar]
  7. Drucker D.B., Lee S.M. 1981; Fatty acid fingerprints of Streptococcus milleri, Streptococcus mitis and related species. International Journal of Systematic Bacteriology 31:219–225
    [Google Scholar]
  8. Facklam R.R. 1984; The major differences in the American and the British Streptococcus taxonomy systems with special reference to Streptococcus milleri . European Journal of Clinical Microbiology 3:91–93
    [Google Scholar]
  9. French G.L., Phillips I., Chinn S. 1981; Reproducible pyrolysis-gas chromatography of micro-organisms with solid stationary phases and isothermal oven temperatures. Journal of General Microbiology 125:347–355
    [Google Scholar]
  10. French G.L., Talsania H., Phillips I. 1985; The use of API-20 STREP for the classification and identification of viridans streptococci. In Recent Advances in Streptococci and Streptococcal Diseases p. 114 Kimura Y., Kotami S., Shiokawa Y. Edited by England: Reedbooks;
    [Google Scholar]
  11. Goodfellow M., Minnikin D.E. 1985; Introduction to chemosystematics. In Chemical Methods in Bacterial Systematics pp. 1–15 Goodfellow M., Minnikin D. E. Edited by London & New York: Academic Press;
    [Google Scholar]
  12. Guthof O. 1956; Ueber pathogene “vergrunede Streptokokken”, Streptokokken-Befunde bei dento-genen Abszessen und Infiltraten im Bereich der Mundhohle. Zentrablatt für Bakteriologie, Parasiten-kunde, Infectionskrankheiten und Hygiene (Abteilung I, Originale A) 166:553–564
    [Google Scholar]
  13. Klopfenstein W.E. 1971; On methylation of unsaturated acids using boron trihalide-methanol reagents. Journal of Lipid Research 12:773–776
    [Google Scholar]
  14. Knight R.G., Shlaes D.M. 1988; Physiological characteristics and deoxyribonucleic acid relatedness of Streptococcus intermedius strains. International Journal of Systematic Bacteriology 38:19–24
    [Google Scholar]
  15. Labbe M., Van Der Auwera P., Glupczynski Y., Crockaert F., Yourassowsky E. 1985; Fatty acid composition of Streptococcus milleri . European Journal of Clinical Microbiology 4:391–393
    [Google Scholar]
  16. Moss C.W., Nunez-Montiel O.L. 1982; Analysis of short chain acids from bacteria by gas-liquid chromatography with a fused silica capillary column. Journal of Clinical Microbiology 15:308–311
    [Google Scholar]
  17. Moss C.W., Lambert M.A., Merwin W.H. 1974; Comparison of rapid methods for analysis of bacterial fatty acids. Applied Microbiology 28:80–85
    [Google Scholar]
  18. Poole P.M., Wilson G. 1979; Occurrence and cultural features of Streptococcus milleri in various body sites. Journal of Clinical Pathology 32:764–768
    [Google Scholar]
  19. Ruoff K. 1988; Streptococcus anginosus (“Streptococcus milleri”), the unrecognised pathogen. Clinical Microbiology Reviews 1:102–108
    [Google Scholar]
  20. Ryhage R., Stenhagen E. 1960; Mass spectrometry in lipid research. Journal of Lipid Research 1:361–390
    [Google Scholar]
  21. Skerman V.B.D., Mcgowan V., Sneath P.H.A. 1980; Approved lists of bacterial names. International Journal of Systematic Bacteriology 60:225–420
    [Google Scholar]
  22. Sneath P.H.A. 1985; Future of numerical taxonomy. In Computer Assisted Bacterial Systematics pp. 415–431 Goodfellow M., Jones D., Priest F. G. Edited by London & New York:: Academic Press.;
    [Google Scholar]
  23. Veralli R. 1986; The Streptococcus milleri group. Infection Control 7:558–560
    [Google Scholar]
  24. Welborn P., Hadley W.K., Newbrun E., Yajko D. 1983; Characterization of strains of viridans streptococci by deoxyribonucleic acid hybridization and physiological tests. International Journal of Systematic Bacteriology 33:293–299
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-4-831
Loading
/content/journal/micro/10.1099/00221287-135-4-831
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error