1887

Abstract

Effects of Na on respiratory NADH oxidase were studied in membranes prepared from Gram-negative marine bacteria belonging to the genera and NADH oxidases of 9 out of the 10 strains examined were found to require Na for maximum activity. Without exception, the Na-dependent site was located at the level of the NADH: quinone oxidoreductase region of the respiratory chain and was highly sensitive to 2-heptyl-4-hydroxyquinoline--oxide (HQNO). NADH dehydrogenases in the Na-dependent segment were sensitive to Ag and able to oxidize reduced nicotinamide hypoxanthine dinucleotide as a substrate. Since the NADH dehydrogenase was neither dependent on Na nor sensitive to HQNO, it was suggested that reduction of quinone by the NADH: quinone oxidoreductase involves the formation of an intermediate, presumably semiquinone, and that electron transfer from the intermediate requires Na and is sensitive to HQNO. These results showed a striking similarity to those obtained with the Na-dependent NADH: quinone oxidoreductase, the Na pump, of , indicating that this enzyme is widely distributed among Gram-negative marine bacteria and shares common properties.

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1989-03-01
2022-01-17
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References

  1. Asano M., Hayashi M., Unemoto T., Tokuda H. 1985; Ag+-sensitive NADH dehydrogenase in the Na+-motive respiratory chain of the marine bacterium Vibrio alginolyticus . Agricultural and Biological Chemistry 49:2813–2817
    [Google Scholar]
  2. Hayashi M., Unemoto T. 1984; Characterization of the Na+-dependent respiratory chain NADH :quinone oxidoreductase of the marine bacterium, Vibrio alginolyticus, in relation to the primary Na+ pump. Biochimica et biophysica acta 767:470–478
    [Google Scholar]
  3. Hayashi M., Unemoto T. 1986; FAD and FMN flavoproteins participate in the sodium-transport respiratory chain NADH:quinone reductase of a marine bacterium, Vibrio alginolyticus . FEBS Letters 202:327–330
    [Google Scholar]
  4. Ingledew W. J., Poole R. K. 1984; The respiratory chain of Escherichia coli . Microbiological Reviews 48:222–271
    [Google Scholar]
  5. Ken-Dror S., Preger R., Avi-Dor Y. 1986a; Functional characterization of the uncoupler-insensitive Na+ pump of the halotolerant bacterium, Ba1 . Archives of Biochemistry and Biophysics 244:122–127
    [Google Scholar]
  6. Ken-Dror S., Lanyi J. K., Schobert B., Silver B., Avi-Dor Y. 1986b; An NADH:quinone oxidoreductase of the halotolerant bacterium Ba, is specifically dependent on sodium ion. Archives of Biochemistry and Biophysics 244:766–772
    [Google Scholar]
  7. Matsushita K., Ohnishi T., Kaback H. R. 1987; NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain. Biochemistry 26:7732–7737
    [Google Scholar]
  8. Rieske J. S. 1967; Preparation and properties of reduced coenzyme Q-cytochrome c reductase (complex III of the respiratory chain). Methods in Enzymology 10:239–245
    [Google Scholar]
  9. Tokuda H. 1983; Isolation of Vibrio alginolyticus mutants defective in the respiration-coupled Na+ pump. Biochemical and Biophysical Research Communications 114:113–118
    [Google Scholar]
  10. Tokuda H. 1986; Sodium translocation by NADH oxidase of Vibrio alginolyticus: isolation and characterization of the sodium pump-defective mutants. Methods in Enzymology 125:520–530
    [Google Scholar]
  11. Tokuda H., Unemoto T. 1981; A respiration-dependent primary sodium extrusion system functioning at alkaline pH in the marine bacterium Vibrio alginolyticus . Biochemical and Biophysical Research Communications 102:265–271
    [Google Scholar]
  12. Tokuda H., Unemoto T. 1982; Characterization of the respiration-dependent Na+ pump in the marine bacterium Vibrio alginolyticus . Journal of Biological Chemistry 257:10007–10014
    [Google Scholar]
  13. Tokuda H., Unemoto T. 1983; Growth of a marine Vibrio alginolyticus and moderately halo- philic V. costicola becomes uncoupler-resistant when the respiration-dependent Na+ pump functions. Journal of Bacteriology 156:636–643
    [Google Scholar]
  14. Tokuda H., Unemoto T. 1984; Na+is translocated at NADH :quinone oxidoreductase segment in the respiratory chain of Vibrio alginolyticus . Journal of Biological Chemistry 259:7785–7790
    [Google Scholar]
  15. Tokuda H., Nakamura T., Unemoto T. 1981; Potassium ion is required for the generation of pH- dependent membrane potential and ApH by the marine bacterium Vibrio alginolyticus . Biochemistry 20:4198–4203
    [Google Scholar]
  16. Tokuda H., Udagawa T., Unemoto T. 1985; Generation of the electrochemical potential of Na+ by the Na+-motive NADH oxidase in inverted membrane vesicles of Vibrio alginolyticus . FEBS Letters 183:95–98
    [Google Scholar]
  17. Tokuda H., Udagawa T., Asano M., Yamamoto T., Unemoto T. 1987; Conjugation-dependent recovery of the Na+ pump in a mutant of Vibrio alginolyticus lacking three subunits of the Na+ pump. FEBS Letters 215:335–338
    [Google Scholar]
  18. Tokuda H., Asano M., Shimamura Y., Unemoto T., Sugiyama S., Imae Y. 1988; Roles of the respiratory Na+ pump in bioenergetics of Vibrio alginolyticus . Journal of Biochemistry 103:650–655
    [Google Scholar]
  19. Tsuchiya T., Shinoda S. 1985; Respiration-driven Na+ pump and Na+ circulation in Vibrio parahaemolyticus . Journal of Bacteriology 162:794–798
    [Google Scholar]
  20. Udagawa T., Unemoto T., Tokuda H. 1986; Generation of Na+ electrochemical potential by the Na+-motive NADH oxidase and Na+/H+ antiport system of a moderately halophilic Vibrio costicola . Journal of Biological Chemistry 261:2616–2622
    [Google Scholar]
  21. Unemoto T., Hayashi M., Hayashi M. 1981; Partial purification and properties of respiratory chain-linked L-glycerol 3-phosphate dehydrogenase from a marine bacterium, Vibrio alginolyticus . Journal of Biochemistry 90:619–628
    [Google Scholar]
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