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Volume 135, Issue 3

Degradation of Fungal Cell Walls by Lytic Enzymes of Free

Abstract

In tests, two strains of failed to parasitize colonies of f. sp. and f. sp. However, these strains were strongly mycoparasitic on and When grown in liquid cultures containing laminarin, chitin or fungal cell walls as sole carbon sources, both strains of released, 1-3--glucanase and chitinase into the medium. Higher levels of these enzymes were induced in strain T-203 than in T-35 by hyphal cell walls of When the lytic enzymes produced by T-35 were incubated with hyphal cell walls of the test fungi, more glucose and -acetyl--glucosamine was released from cell walls of and than from those of Treatment of cell walls with 2 -NaOH, protease or trypsin prior to their incubation with the lytic enzymes of significantly increased the release of glucose and -acetyl--glucosamine. The effect of these treatments on and cell walls was much lower. These results suggest that proteins in the cell walls of may make these walls more resistant than those of or to degradation by extracellular enzymes of .

  • Received:
  • Accepted:
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  • Published Online:
© Society for General Microbiology, 1989
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1989-03-01
2024-04-19
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References

  1. Artigues M., Davet P. 1984; Activités (1-3) glucanisque et chitinisque de quelques champignons, en relation avec leur aptitude à detruire les sclerotes deCorticium rolfsii dans la terre sterile. Soil Biology and Biochemistry 16:527–538
     [Google Scholar]
  2. Barran L.R., Schneider E.F., Wood P.J., Madhosingh C., Miller W.R. 1975; Cell wall of Fusarium sulphureum. I. Chemical composition of the hyphal wall. Biochimica et biophysica acta 392:148–158
     [Google Scholar]
  3. Bull A.T., Chesters C.G.C. 1966; The biochemistry of laminarin and the nature of laminar-inase. Advances in Enzymology 28:325–364
     [Google Scholar]
  4. Chet I., Huttermann A. 1980; Chemical composition of hyphal walls of Fomes annosus . European Journal of Forest Pathology 10:65–70
     [Google Scholar]
  5. Cocking E.C., Yemm E.W. 1954; Estimation of amino acids by ninhydrin. Biochemical Journal 58:xii
     [Google Scholar]
  6. Dennis C., Webster J. 1971; Antagonistic properties of species-groups of Trichoderma. III. Hyphal interaction. Transactions of the British Mycological Society 57:363–369
     [Google Scholar]
  7. Elad Y., Chet I. 1983; Improved selective media for isolation of Trichoderma spp. or Fusarium spp. Phytoparasitica 11:55–58
     [Google Scholar]
  8. Elad Y., Chet I., Katan J. 1980; Trichoderma harzianum : a biocontrol agent effective against Sclerotium rolfsii and Rhizoctonia solani . Phytopathology 70:119–121
     [Google Scholar]
  9. Elad Y., Chet I., Henis Y. 1982; Degradation of plant pathogenic fungi by Trichoderma harzianum . Canadian Journal of Microbiology 28:719–725
     [Google Scholar]
  10. Elad Y., Lifshitz R., Baker R. 1985; Enzymatic activity of the mycoparasite Pythium nunn during interaction with host and non-host fungi. Physiological Plant Pathology 27:131–148
     [Google Scholar]
  11. Hadar Y., Chet I., Henis Y. 1979; Biological control of Rhizoctonia solani damping-off with wheat bran culture of Trichoderma harzianum . Phytopathology 69:64–68
     [Google Scholar]
  12. Henis Y., Chet I. 1975; Microbiological control of plant pathogens. Advances in Applied Microbiology 19:85–111
     [Google Scholar]
  13. Laborda F., Garcia-Acha I., Uruburu F., Villanueva J.R. 1974; Structure of the conidial wall of Fusarium culmorum . Transactions of the British Mycological Society 62:557–566
     [Google Scholar]
  14. Lynch J.M. 1987; In vitro identification of Trichoderma harzianum as a potential antagonist of plant pathogens. Current Microbiology 16:49–53
     [Google Scholar]
  15. Mitchell R., Alexander M. 1963; Lysis of soil fungi by bacteria. Canadian Journal of Microbiology 15:689–696
     [Google Scholar]
  16. Monreal J., Reese E.T. 1969; The chitinase of Serratia marcescens . Canadian Journal of Microbiology 15:689–696
     [Google Scholar]
  17. Nash S.M., Snyder W.C. 1962; Quantitative estimations by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology 52:567–572
     [Google Scholar]
  18. Okon Y., Chet I., Henis Y. 1973; Effect of lactose, ethanol and cycloheximide on the translocation pattern of radioactive compounds and on sclerotium formation in Sclerotium rolfsii . Journal of General Microbiology 74:251–258
     [Google Scholar]
  19. Reese E.T., Mandels M. 1959; β -l,3-Glucanase in fungi. Canadian Journal of Microbiology 5:173–185
     [Google Scholar]
  20. Reissig J.L., Strominger J.L., Leloir L.F. 1959; A modified colorimetric method for the estimation of N-acetyl sugars. Journal of Biological Chemistry 217:959–962
     [Google Scholar]
  21. Ridout C.J., Coley-Smith J.R., Lynch J.M. 1986; Enzyme activity and electrophoretic profile of extracellular protein induced by cell walls of Rhizoctonia solani . Journal of General Microbiology 132:2345–2352
     [Google Scholar]
  22. Rodriguez-Kabana R., Godoy G., Morgan-Jones G., Shelby R.A. 1983; The determination of soil chitinase activity: conditions for assay and ecological studies. Plant and Soil 75:95–106
     [Google Scholar]
  23. Schneider E.F., Barran L.R., Wood P.J., Siddiqui I.R. 1977; Cell wall of Fusarium sulphureum. II. Chemical composition of the conidial and chlamydospore walls. Canadian Journal of Microbiology 23:763–769
     [Google Scholar]
  24. Sedmak J.J., Grossberg S.E. 1977; A rapid, sensitive and versatile assay for protein using Coomassie brilliant blue G250. Analytical Biochemistry 79:544–552
     [Google Scholar]
  25. Sivan A., Chet I. 1986; Biological control of Fusarium spp. in cotton, wheat and muskmelon by richoderma harzianum . Journal of Phytopathology 116:39–47
     [Google Scholar]
  26. Sivan A., Ucko O., Chet I. 1987; Biological control of Fusarium crown rot of tomato by Trichoderma harzianum under field conditions. Plant Disease 71:587–592
     [Google Scholar]
  27. Sivan A., Chet I. 1989; The possible role of competition between Trichoderma harzianum and Fusarium oxysporum on rhizosphere colonization. Phytopathology (in the Press).
    [Google Scholar]
  28. Tokimoto K. 1982; Lysis of the mycelium ofLentinus edodes caused by mycolytic enzymes of Trichoderma harzianum when the two fungi were in antagonistic state. Transactions of the Mycological Society of Japan 23:13–20
     [Google Scholar]
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Degradation of Fungal Cell Walls by Lytic Enzymes of Trichoderma harzianum
Microbiology 135, 675 (1989); https://doi.org/10.1099/00221287-135-3-675
/content/journal/micro/10.1099/00221287-135-3-675
/content/journal/micro/10.1099/00221287-135-3-675
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