1887

Abstract

The use of phage SP50 as marker for cell wall containing teichoic acid in showed clear differences in the rates at which new wall material becomes exposed at polar and cylindrical regions of the wall, though the poles were not completely conserved. Following transition from phosphate limitation to conditions that permitted synthesis of teichoic acid, old polar caps fairly rapidly incorporated enough teichoic acid to permit phage binding. Electron microscopy suggested that the new receptor material spread towards the tip of the pole from cylindrical wall so that phages bound to an increasing proportion of the pole area until only the tip lacked receptor. Eventually, receptor was present over the whole polar surface. Direct electron microscopic staining of bacteria collected during transitions between magnesium and phosphorus limitations showed that new material was incorporated at the inner surface of polar wall and later became exposed at the outer surface by removal of overlying older wall. The apparent partial conservation of the pole reflected a slower degradation of the overlying outer wall at the pole than at the cylindrical surface, the rate being graded towards the tip of the pole. The relative proportions of the new wall material incorporated into polar and cylindrical regions differed in bacteria undergoing transitions that were accompanied by upshift or downshift in growth rate. These differences can be explained on the basis that growth rate affected the rate of synthesis of cylindrical but not septal wall.

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/content/journal/micro/10.1099/00221287-135-3-657
1989-03-01
2022-01-29
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References

  1. Anderson A.J., Green R.S., Sturman A.J., Archibald A.R. 1978; Cell wall assembly in Bacillus subtilis: location of wall material incorporated during pulsed release of phosphate limitation, its accessibility to bacteriophages and concanavalin A and its susceptibility to turnover. Journal of Bacteriology 136:886–899
    [Google Scholar]
  2. Archibald A.R. 1976; Cell wall assembly in Bacillus subtilis: development of bacteriophage binding properties as a result of the pulsed incorporation of teichoic acid. Journal of Bacteriology 127:956–960
    [Google Scholar]
  3. Archibald A.R., Coapes H.E. 1976; Bacteriophage SP50 as a marker for cell wall growth in Bacillus subtilis . Journal of Bacteriology 125:1195–1206
    [Google Scholar]
  4. Burdett I.D.J., Higgins M.L. 1978; Study of pole assembly in Bacillus subtilis by computer reconstruction of septal growth zones seen in central longitudinal sections of cells. Journal of Bacteriology 133:959–971
    [Google Scholar]
  5. Burman L.G., Park J.T. 1983; Evidence for multi-site growth of Escherichia coli murein involving concomitant endopeptidase and transpeptidase activity. Journal of Bacteriology 156:386–392
    [Google Scholar]
  6. Chung K.L., Hawirko R.Z., Isaac P.K. 1964; Cell wall replication. 1. Cell wall growth of Bacillus cereus and Bacillus megaterium . Canadian Journal of Microbiology 10:43–48
    [Google Scholar]
  7. Clarke-Sturman A.J., Archibald A.R. 1982; Cell wall turnover in phosphate and potassium limited cultures of Bacillus subtilis W23. Archives of Microbiology 131:375–379
    [Google Scholar]
  8. Doyle R.J., Mobley H.L.T., Joliffe L.K., Streips U.N. 1980; Restricted turnover of the cell wall in Bacillus subtilis . Current Microbiology 5:19–22
    [Google Scholar]
  9. Ellwood D.C., Tempest D.W. 1972; Effect of environment on bacterial wall content and composition. Advances in Microbial Physiology 7:83–117
    [Google Scholar]
  10. Fan D.P., Beckman B.E. 1973; Structural difference between walls from hemispherical caps and partial septa of Bacillus subtilis . Journal of Bacteriology 114:790–797
    [Google Scholar]
  11. Fan D.P., Beckman B.E., Beckman M.M. 1974; Cell wall turnover at the hemispherical caps of Bacillus subtilis . Journal of Bacteriology 117:1330–1334
    [Google Scholar]
  12. Fan D.P., Beckman B.E., Gardner-Eckstrom H.L. 1975; Mode of cell wall synthesis in Grampositive bacteria. Journal of Bacteriology 123:1157–1162
    [Google Scholar]
  13. Goodell E.W., Schwarz U. 1983; Cleavage and resynthesis of peptide cross-bridges in Escherichia coli murein. Journal of Bacteriology 156:136–140
    [Google Scholar]
  14. Hughes R.C., Stokes E. 1971; Cell wall growth in Bacillus licheniformis followed by immunofluorescence with mucopeptide-specific antiserum. Journal of Bacteriology 106:694–696
    [Google Scholar]
  15. Kirchner G., Koch A.L., Doyle R.J. 1984; Energised membrane regulates cell pole formation in Bacillus subtilis . FEMS Microbiology Letters 24:143–147
    [Google Scholar]
  16. Koch A.L. 1983; The surface stress theory of microbial morphogenesis. Advances in Microbial Physiology 24:301–366
    [Google Scholar]
  17. Koch A.L., Doyle R.J. 1986; Growth strategy for the Gram-positive rod. FEMS Microbiology Reviews 32:247–254
    [Google Scholar]
  18. Lang W.K., Glassey K., Archibald A.R. 1982; Influence of phosphate supply on teichoic acid and teichuronic acid content of Bacillus subtilis cell walls. Journal of Bacteriology 151:367–375
    [Google Scholar]
  19. Merad T., Archibald A.R., Hancock I.C., Harwood C.R., Hobot J.A. 1989; Cell wall assembly in Bacillus subtilis : visualization of old and new wall material by electron microscopic examination of samples stained selectively for teichoic acid and teichuronic acid. Journal of General Microbiology 135:645–655
    [Google Scholar]
  20. Mobley H.L.T., Koch A.L., Doyle R.J., Streips U.N. 1984; Insertion and fate of the cell wall in Bacillus subtilis . Journal of Bacteriology 158:169–179
    [Google Scholar]
  21. Sargent M.G. 1975; Control of cell length in Bacillus subtilis . Journal of Bacteriology 123:7–19
    [Google Scholar]
  22. Sturman A.J., Archibald A.R. 1978; Conservation of phage receptor material at the polar caps of Bacillus subtilis W23. FEMS Microbiology Letters 4:255–259
    [Google Scholar]
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