1887

Abstract

Two fertility-inhibition functions which reduce R388 (IncW) transfer were detected on RP1 (60 kb, IncP). The respective genes, and , were mapped by transposon insertion mutagenesis to the regions between coordinates 32·8 to 31·7 kb (), and 59·8 to 0·8 kb (). The function occurs in a non-essential region of RP1 whereas is straddled by essential plasmid-maintenance and host-range determinants and apparently coincides (or overlaps) with the gene for tellurite-resistance.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-3-499
1989-03-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/3/mic-135-3-499.html?itemId=/content/journal/micro/10.1099/00221287-135-3-499&mimeType=html&fmt=ahah

References

  1. Arwas R. 1982 A study of transposons and transpo- son-mediated genetic interactions in Pseudomonas aeruginosa. PhD thesis, La Trobe University, Australia
    [Google Scholar]
  2. Bennett P.M., Grinsted J., Choi C.L., Richmond M.H. 1978; Characterisation of Tn501, a transposon determining resistance to mercuric ions. Molecular and General Genetics 159:101–106
    [Google Scholar]
  3. Bradley D.E. 1985; Detection of tellurite-resistance determinants in IncP plasmids. Journal of General Microbiology 131:3135–3137
    [Google Scholar]
  4. Elliott S. 1983 Interactions of transposons Tn504 and Tn801 with DNA molecules in Pseudomonas aeruginosa PAO. MSc thesis, La Trobe University, Australia.
    [Google Scholar]
  5. Grinsted J., Saunders J.R., Ingram L.C., Sykes R.B., Richmond M.H. 1972; Properties of an R factor which originated in Pseudomonas aeruginosa 1822. Journal of Bacteriology 110:529–537
    [Google Scholar]
  6. Hedges R.W., Datta N., Coetzee J.N., Dennison S. 1973; R factors from Proteus morganii . Journal of General Microbiology 77:249–259
    [Google Scholar]
  7. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Mccombie W., Hansen J.B., Zylstra G.J., Maurer B., Olsen R.H. 1983; Pseudomonas streptomycin resistance transposon associated with R-plasmid mobilization. Journal of Bacteriology 155:40–48
    [Google Scholar]
  9. Sanchez J., Bennett P.M., Richmond M.H. 1982; Expression of eltB, the gene encoding the B sub-unit of the heat-labile enterotoxin of Escherichia coli, when cloned in pACYC184. FEMS Microbiology Letters 14:1–5
    [Google Scholar]
  10. Stanisich V.A., Bennett P.M. 1976; The properties of hybrids formed between the P-group plasmid RP1 and various plasmids from Pseudomonas aeruginosa . Molecular and General Genetics 149:217–223
    [Google Scholar]
  11. Summers A.O., Jacoby G.A. 1977; Plasmid-determined resistance to tellurite compounds. Journal of Bacteriology 129:276–281
    [Google Scholar]
  12. Taylor D.E., Bradley D.E. 1987; Location on RP4 of a tellurite resistance determinant not normally expressed in IncPα plasmids. Antimicrobial Agents and Chemotherapy 31:823–825
    [Google Scholar]
  13. Thomas C.M., Smith C.A. 1987; Incompatibility group P plasmids: genetics, evolution, and use in genetic engineering. Annual Review of Microbiology 41:77–101
    [Google Scholar]
  14. Wallace L.J., Ward J.M., Richmond M.H. 1981; The location of sequences of TnA required for the establishment of transposition immunity. Molecular and General Genetics 184:80–86
    [Google Scholar]
  15. Yusoff K., Stanisich V.A. 1984; Location of a function on RP1 that fertility inhibits Inc W plasmids. Plasmid 11:178–181
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-3-499
Loading
/content/journal/micro/10.1099/00221287-135-3-499
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error