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The growth factor requirements and other properties of two strains of avian haemophili, labelled as ‘Haemophilus gallinarum’(an X- and V-factor-dependent organism) and stored since the 1940s and 1950s, were examined. Both strains were X-factor independent and V-factor dependent and possessed the typical biochemical serological and pathological properties of H. paragallinarum. In experiments repeating the tests used in the 1930s that reported the existence of X- and V-factor-dependent avian haemophili, we found that the methodology used resulted in reference strains of H. paragallinarum (X-factor independent and V-factor dependent) appearing to be X- and V-factor dependent. It is likely that the early descriptions of the aetiological agent of infectious coryza as an X- and V-factor-dependent organism were incorrect.
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