1887

Abstract

Five representative bacterial strains resembling , chosen from among forty strains isolated by three different procedures, were characterized by phenotypic and genotypic analyses. They were shown to belong to the species , or to a distinct, but closely related, cluster. In contrast to the initial isolates of this species, most of the new strains were able to fix N, as shown by their growth in nitrogen-free medium and by the acetylene reduction test. Moreover, DNA regions of these new strains were homologous to genes, confirming the presence of nitrogenase genes. The genes are probably chromosomally located, since no plasmid was detected in these strains. The presence of complete but defective genes in GA3 (the type strain, which does not fix N) was excluded, as no homology was found between genes and GA3 total DNA. The isolation conditions seem to determine the selection of N-fixing strains, since selection for H-chemolithoautotrophs gave no N-fixing isolates, whereas H-chemolithoheterotrophic selection conditions (with combined nitrogen) yielded a majority of N fixers.

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1989-02-01
2024-12-07
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References

  1. Aragno M., Schlegel H.G. 1981; The hydrogen- oxidizing bacteria. In The Prokaryotes, a Handbook on Habitats, Isolation and Identification of Bacteria pp 865–893 Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Edited by Berlin/Heidel-berg/New York: Springer-Verlag;
    [Google Scholar]
  2. Aragno M., Walther-Mauruschat A., Meyer F., Schlegel H.G. 1977; Micromorphology of Gram-negative hydrogen bacteria. I. Cell morphology and flagellation. Archives of Microbiology 114:93–100
    [Google Scholar]
  3. Auling G., Reh M., Lee C. M., Schlegel H. G. 1978; Pseudomonas pseudoflava, a new species of hydrogen-oxidizing bacteria : its differentiation from Pseudomonas flava and other yellow-pigmented, Gram-negative, hydrogen-oxidizing species. International Journal of Systematic Bacteriology 28:82–95
    [Google Scholar]
  4. Barraquio W.L., Padre B.C., Watanabe I., Knowles R. 1986; Nitrogen fixation byPseudomonas saccharophila Doudoroff ATCC 15946. Journal of General Microbiology 132:237–241
    [Google Scholar]
  5. Berlier J.M., Lespinat P.A. 1978; Relationship between nitrogenase and hydrogenase activity in a rhizosphere diazotroph. FEMS Microbiology Letters 3:187–190
    [Google Scholar]
  6. Cannon F.C., Riedel G.E., Ausubel F.M. 1979; Overlapping sequences ofKlebsiella pneumoniae nif DNA cloned and characterized. Molecular and General Genetics 174:59–66
    [Google Scholar]
  7. Colman A., Byers M.J., Primrose S.B., Lyons A. 1978; Rapid purification of plasmid DNAs by hydroxyapatite chromatography. European Journal of Biochemistry 91:303–310
    [Google Scholar]
  8. Dugnani L., Wyrsch I., Gandolla M., Aragno M. 1986; Biological oxidation of hydrogen in soils flushed with a mixture of H2, CO2, O2 and N2 . FEMS Microbiology Ecology 38:347–351
    [Google Scholar]
  9. Elmerich C., Dreyfus B.L., Reysset G., Aubert J.P. 1982; Genetic analysis of nitrogen fixation in a tropical fast-growingRhizobium . EMBO Journal 1:499–503
    [Google Scholar]
  10. Gerstenberg C., Friedrich B., Schlegel H.G. 1982; Physical evidence for plasmids in autotrophic, especially hydrogen-oxidizing bacteria. Archives of Microbiology 133:90–96
    [Google Scholar]
  11. Hennecke H., Kaluza K., Thöny B., Fuhrmann M., Ludwig W., Stackebrandt E. 1985; Concurrent evolution of nitrogenase genes and 16S rRNA inRhizobium species and other nitrogen fixing bacteria. Archives of Microbiology 142:342–348
    [Google Scholar]
  12. Jenni B., Stutz E. 1978; Physical mapping of the ribosomal DNA region ofEuglena gracilis chloro-plast DNA. European Journal of Biochemistry 88:127–134
    [Google Scholar]
  13. Jenni B., Fasnacht M., Stutz E. 1981; The multiple copies of theEuglena gracilis chloroplast genome are not uniform in size. FEBS Letters 125:175–179
    [Google Scholar]
  14. Jenni B., Aragno M., Wiegel J.K.W. 1987; Numerical analysis and DNA :DNA hybridization studies onXanthobacter and emendation of Xantho-bacter flavus . Systematic and Applied Microbiology 9:247–253
    [Google Scholar]
  15. Jenni B., Realini L., Aragno M., Tamer A.Ü. 1988; Taxonomy of non H2-lithotrophic, oxalate- oxidizing bacteria related to Alcaligenes eutrophus . Systematic and Applied Microbiology 10:126–133
    [Google Scholar]
  16. Johnson J.L. 1984; Nucleic acids in bacterial classification. In Bergey’s Manual of Systematic Bacteriology 252: pp 8–11 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  17. Krotzky A., Werner D. 1987; Nitrogen fixation inPseudomonas stutzeri . Archives of Microbiology 147:48–57
    [Google Scholar]
  18. Malik K.A., Schlegel H.G. 1980; Enrichment and isolation of new nitrogen-fixing hydrogen bacteria. FEMS Microbiology Letters 8:101–104
    [Google Scholar]
  19. Mandel M., Igambi L., Bergendahl J., Dodson M.L., Scheltgen J.R. 1970; Correlation of melting temperature and cesium chloride buoyant density of bacterial desoxyribonucleic acid. Journal of Bacteriology 101:333–338
    [Google Scholar]
  20. Mazur B.J., Rice D., Haselkorn R. 1980; Identification of blue-green algal nitrogen fixation genes by using heterologous DNA hybridization probes. Proceedings of the National Academy of Sciences of the United States of America 77:186–190
    [Google Scholar]
  21. Oakley C. J., Murrell J.C. 1988; nifH genes in the obligate methane oxidizing bacteria. FEMS Microbiology Letters 49:53–57
    [Google Scholar]
  22. Owen R.J., Hill L.R. 1979; The estimation of base compositions, base pairing and genome sizes of bacterial deoxyribonucleic acids. Technical Series of the Society for Applied Bacteriology 14:277–296
    [Google Scholar]
  23. Palleroni N.J. 1984; Gram-negative aerobic rods and cocci.Pseudomonadaceae . In Bergey’s Manual of Systematic Bacteriology 1: pp 141–199 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  24. Possot O., Henry M., Sibold L. 1986; Distribution of DNA sequences homologous tonifH among archaebacteria. FEMS Microbiology Letters 34:173–177
    [Google Scholar]
  25. Postgate J., Kent H.M., Robson R.L. 1986; DNA from diazotrophicDesulfovibrio strains is homologous to Klebsiella pneumoniae structural nifDNA and can be chromosomal or plasmid-borne. FEMS Microbiology Letters 33:159–163
    [Google Scholar]
  26. Ruttenberg-Barnum S., Gendel S.M. 1985; Organization of nitrogen fixation genes in a nonheterocystous, filamentous cyanobacterium. FEMS Microbiology Letters 29:339–342
    [Google Scholar]
  27. Schweizer C., Aragno M. 1975; Etude des hydrogenobacteries dans un petit lac, Le Loclat ou lac de Saint-Blaise. Bulletin de la Société Neuchȃte- loise des Sciences Naturelles 98:79–87
    [Google Scholar]
  28. Schmidt K., Liaaen-Jensen S., Schlegel H.G. 1963; Die Carotenoide der Thiorhodaceae. Archiv für Mikrobiologie 46:117–126
    [Google Scholar]
  29. Sneath P.H.A. 1984; Numerical taxonomy. In Bergey’s Manual of Systematic Bacteriology 265: pp 5–7 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  30. Walther-Mauruschat A., Aragno M., Mayer M., Schlegel H.G. 1977; Micromorphology of Gram-negative hydrogen bacteria. II. Cell envelope, membranes, and cytoplasmic inclusions. Archives of Microbiology 114:101–110
    [Google Scholar]
  31. Watanabe I., So R., Ladha J.K., Katayama-Fujimura Y., Huraishi H. 1987; A new nitrogen-fixing species of pseudomonad:Pseudomonas diazotrophicus sp. nov. isolated from the root of wetland rice. Canadian Journal of Microbiology 33:670–678
    [Google Scholar]
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