1887

Abstract

The outer-membrane protein (OMP) profile of grown under iron-replete and iron-restricted conditions was studied by polyacrylamide gel electrophoresis and immunoblotting. A serotype 1 isolate induced the synthesis of a new 77000 OMP in iron-restricted media while two other proteins of 100000 and 71000 were synthesized in increased amounts. None of these proteins were peptidoglycan-associated or heat-modifiable, and only the 100000 protein showed some degree of disulphide cross-linking. Kinetic analysis revealed that the iron-repressible proteins appeared in the outer membrane within 15 min of establishment of iron-restricted conditions. Analysis of isolates representing serotypes 1 to 12 showed that iron-repressible OMPs of 77000 and 71000 could be induced in all 12 serotypes but that there was some variability in the expression of the 100000 protein. Immunoblotting of OMPs with convalescent sera from -ifected calves indicated that antibodies directed against all three iron-repressible OMPs were present, suggesting that these proteins were expressed .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-2-435
1989-02-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/2/mic-135-2-435.html?itemId=/content/journal/micro/10.1099/00221287-135-2-435&mimeType=html&fmt=ahah

References

  1. Adlam C., Knights J.M., Mugridge A., Lindon J.C., Baker P.R.W., Beesley J.E., Spacey B., Craig G., Nagy L.K. 1984; Purification, characterization and immunological properties of the serotype-specific capsular polysaccharide of Pasteurella haemolytica (serotype Al) organisms. Journal of General Microbiology 130:2415–2426
    [Google Scholar]
  2. Aisen P., Listowski I. 1980; Iron transport and storage proteins. Annual Review of Biochemistry 49:357–393
    [Google Scholar]
  3. Babiuk L.A., Acres S.D. 1984; Model for bovine respiratory disease. In Bovine Respiratory Disease: a Symposium pp. 287–325 Loan R. W. Edited by College Station, Texas: Texas A & M University Press;
    [Google Scholar]
  4. Barenkamp S.J., Munson R. Jr Granoff D.M. 1981; subtyping isolates of Haemophilus influenza type b by outer membrane protein profiles. Journal of Infectious Diseases 143:668–676
    [Google Scholar]
  5. Bezkorovainy A. 1981; Antimicrobial properties of iron-binding proteins. Advances in Experimental Medicine and Biology 135:139–154
    [Google Scholar]
  6. Biberstein E.L., Gills M., Knight H. 1960; Serological types of Pasteurella haemolytica . Cornell Veterinarian 50:283–300
    [Google Scholar]
  7. Black D., Dyer D., Thompson M., Sparling P.F. 1986; Human immune response to iron- repressible outer membrane proteins of Neisseria meningitidis . Infection and Immunity 54:710–713
    [Google Scholar]
  8. Bolin C.A., Jensen A.E. 1987; Passive immunization with antibodies against iron-regulated outer membrane proteins protects turkeys from Escherichia coli septicemia. Infection and Immunity 55:1239–1242
    [Google Scholar]
  9. Braun V. 1985; The unusual features of the iron transport system of E. coli . Trends in Biochemical Sciences 10:75–78
    [Google Scholar]
  10. Brener C., Devoe I.W. 1982; Expression of a high-affinity mechanism for acquisition of transferrin iron by Neisseria meningitidis . Infection and Immunity 36:107–113
    [Google Scholar]
  11. Brown M., Anwar H., Lambert P. 1984; Evidence that mucoid Pseudomonas aeruginosa in cystic fibrosis lung grows under iron-restricted conditions. FEMS Microbiology Letters 21:113–117
    [Google Scholar]
  12. Bullen J.J. 1981; The significance of iron in infection. Reviews of Infectious Diseases 3:1127–1138
    [Google Scholar]
  13. Chart H., Buck H., Stevenson P., Griffiths E. 1986; Iron regulated outer membrane proteins of Escherichia coli: variation in expression due to the chelator used to restrict the availability of iron. Journal of General Microbiology 132:1373–1378
    [Google Scholar]
  14. Finkelstein R.A., Sciortino C., McIntosh M. 1983; Role of iron in microbe-host interactions. Reviews of Infectious Diseases 5: (suppl.4) 759–777
    [Google Scholar]
  15. Fohn M., Mietzner T., Hubbard T., Morse S., Hook E. III 1987; Human immunoglobulin G antibody response to the major gonococcal iron-regulated protein. Infection and Immunity 55:3065–3069
    [Google Scholar]
  16. Fraser J., Laird S., Gilmour N.J.L. 1982; A new serotype (biotype T) of Pasteurella haemolytica . Research in Veterinary Science 32:127–128
    [Google Scholar]
  17. Gentry M.J., Confer A., Weinberg E., Homer J. 1986; Cytotoxin (leucotoxin) production by Pasteurella haemolytica: requirement for an iron- containing compound. Americal Journal of Veterinary Research 47:1919–1923
    [Google Scholar]
  18. Griffiths E., Stevenson P., Thorpe R., Chart H. 1985; Naturally occurring antibodies in human sera that react with the iron-regulated outer membrane proteins of Escherichia coli . Infection and Immunity 47:808–813
    [Google Scholar]
  19. Holmes R.K., Russel L. 1983; Role of iron in regulating synthesis of bacterial toxins. In Micro-biology-1983 pp. 359–362 Schlessinger D. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Klebba P., McIntosh M., Neilands J.B. 1982; Kinetics of biosynthesis of iron-regulated membrane proteins in Escherichia coli . Journal of Bacteriology 149:880–888
    [Google Scholar]
  21. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  22. Lory S. 1986; Effect of iron on accumulation of exotoxin A-specific mRNA in Pseudomonas aeruginosa . Journal of Bacteriology 168:1451–1456
    [Google Scholar]
  23. MciIntosh M., Chenault S., Earhart C. 1979; Genetic and physiological studies on the relationship between colicin B resistance and ferrienterochelin uptake in Escherichia coli K12. Journal of Bacteriology 137:653–657
    [Google Scholar]
  24. Mickelson P., Blackman E., Sparling P.E. 1982; Ability of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species to obtain iron from lactoferrin. Infection and Immunity 35:915–920
    [Google Scholar]
  25. Morck D., Raybould T., Acres S.D., Babiuk L.A., Nelligan J., Costerton J.W. 1987; Electron microscopic description of glycocalyx and fimbriae on the surface of Pasteurella haemolytica- A1. Canadian Journal of Veterinary Research 51:83–88
    [Google Scholar]
  26. Neilands J.B. 1982; Microbial envelope proteins related to iron. Annual Review of Microbiology 36:285–310
    [Google Scholar]
  27. Rogers H. 1973; Iron-binding catechols and virulence in Escherichia coli . Infection and Immunity 7:445–456
    [Google Scholar]
  28. Sciortino C., Finkelstein R. 1983; Vibrio cholerae expresses iron-regulated outer membrane proteins in vivo . Infection and Immunity 42:990–996
    [Google Scholar]
  29. Shewan P.E., Wilkie B.N. 1982; Cytotoxin of Pasteurella haemolytica acting on bovine leucocytes. Infection and Immunity 35:91–94
    [Google Scholar]
  30. Sigel S., Payne S. 1982; Effect of iron limitation on growth, siderophore production, and expression of outer membrane proteins of Vibrio cholerae . Journal of Bacteriology 150:148–155
    [Google Scholar]
  31. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  32. West S., Sparling P.F. 1985; Response of Neisseria gonorrhoeae to iron limitation: alterations in expression of membrane proteins without apparent siderophore production. Infection and Immunity 47:388–394
    [Google Scholar]
  33. Yates W.D.G. 1982; A review of infectious bovine rhinotracheitis, shipping fever pneumonia, and viral-bacterial synergism in respiratory disease of cattle. Canadian Journal of Comparative Medicine 46:225–263
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-2-435
Loading
/content/journal/micro/10.1099/00221287-135-2-435
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error