1887

Abstract

A clinical isolate of the pathogenic yeast varied in its colony morphology from smooth (o-smooth) to semi-rough type (SRT) and concomitantly lost its virulence for mice. In terms of DNA content, the smooth parent was near triploid when strains of known ploidy were used as references. The SRT variant showed several features characteristic of polyploidy. From the SRT variant, revertant-like smooth (r-smooth) variants with recovered virulence were derived at a frequency of 5 × 10. The results of pulsed-field gel electrophoresis on chromosomal DNA showed changes in patterns of chromosome-sized DNA bands in the SRT variant as well as in r-smooth variants, which correlated with these variations. Correlations between colony morphology, state of ploidy and virulence of this asporogenous yeast are considered.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-2-425
1989-02-01
2021-05-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/2/mic-135-2-425.html?itemId=/content/journal/micro/10.1099/00221287-135-2-425&mimeType=html&fmt=ahah

References

  1. Anderson J.M., Soll D.R. 1987; Unique phenotype of opaque cells in the white-opaque transition of Candida albicans . Journal of Bacteriology 169:5579–5588
    [Google Scholar]
  2. Borst P., Cross S.A.M. 1982; Molecular basis for trypanosome antigenic variation. Cell 29:291–303
    [Google Scholar]
  3. Brown-Thomsen J. 1968; Variability in Candida albicans (Robin) Berkhout. I. Studies on morphology and biochemical activity. Hereditas 60:355–398
    [Google Scholar]
  4. Carle G.F., Olson M.V. 1984; Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Research 12:5647–5664
    [Google Scholar]
  5. Carle G.F., Olson M.V. 1985; An electrophoretic karyotype for yeast. Proceedings of the National Academy of Sciences of the United States of America 82:3756–3760
    [Google Scholar]
  6. Hesse G., Linder R., Krebs D. 1975; Schnelle fluorometrische desoxyribose-spezifische DNS-Bes- timmung an nicht desintegrierten Mikroorganismen mit 3,5-Diaminobenzoesaure (DABA). I. Chemisch- analytische Methodik und Anwendung auf Hefepo- pulationen. Zeitschrift für Allgemeine Mikrobiologie 15:9–18
    [Google Scholar]
  7. Iino T., Kutsukake K. 1983; Flagellar phase variation in Salmonella: a model system regulated by flip-flop DNA inversion. In Nucleic Acid Research: Future Developments, pp 395–406 Mizobuchi K., Watanabe I., Watson J. D. Edited by New York: Academic Press;
    [Google Scholar]
  8. Kilmartin J.V., Adams A.E.M. 1984; Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces . Journal of Cell Biology 98:922–933
    [Google Scholar]
  9. Kwon-Chung K.J., Lehman D., Good C., Magee P.T. 1985; Genetic evidence for role of extracellular proteinase in virulence of Candida albicans . Infection and Immunity 49:571–575
    [Google Scholar]
  10. Macdonald F., Odds F.C. 1983; Virulence for mice of a proteinase-secreting strain of Candida albicans and a proteinase-deficient mutant. Journal of General Microbiology 129:431–438
    [Google Scholar]
  11. Mcginnis M.R. 1980 Laboratory Handbook of Medical Mycology. New York: Academic Press;
    [Google Scholar]
  12. Magee B.B., Magee P.T. 1987; Electrophoretic karyotypes and chromosome numbers in Candida species. Journal of General Microbiology 133:425–430
    [Google Scholar]
  13. Meier J.T., Simon M.I., Barbour A.G. 1985; Antigenic variation associated with DNA rearrangements in a relapsing fever Borrelia . Cell 41:403–409
    [Google Scholar]
  14. Meyer T.F., Mlawer N., So M. 1982; Pilus expression in Neisseria gonorrhoeae involves chromosomal rearrangement. Cell 30:45–52
    [Google Scholar]
  15. Odds F.C., Abbott A.B. 1980; A simple system for the presumptive identification of Candida albicans and differentiation of strains with the species. Sabouraudia 18:301–317
    [Google Scholar]
  16. Riggsby W.S., Torres-Bauza L.J., Wills J.W., Townes T.M. 1982; DNA content, kinetic complexity, and the ploidy question in Candida albicans . Molecular and Cellular Biology 2:853–862
    [Google Scholar]
  17. Roeder G.S. 1983; Unequal crossing-over between yeast transposable elements. Molecular and General Genetics 190:117–121
    [Google Scholar]
  18. Rubin G.M. 1983; Dispersed repetitive DNAs in Drosophila . In Mobile Genetic Elements, pp 329–361 Shapiro J. A. Edited by Orlando: Academic Press;
    [Google Scholar]
  19. Scherer S., Stevens D.A. 1988; A Candida albicans dispersed, repeated gene family and its epidemiologic applications. Proceedings of the National Academy of Sciences of the United States of America 85:1452–1456
    [Google Scholar]
  20. Schwartz D.C., Cantor C.R. 1982; Separation of yeast chromosome-sized DNAs by pulse field gradient gel electrophoresis. Cell 37:67–75
    [Google Scholar]
  21. Shimidzu K., Kondoh Y., Tanaka K. 1987; Proteinase production and pathogenicity of Candida albicans. I. Invasion into chorioallantoic membrane by C. albicans strains of different proteinase activity. Microbiology and Immunology 31:1045–1060
    [Google Scholar]
  22. Simon M., Zieg M., Silverman M., Mandel G., Doolittle R. 1980; Phase variation: evolution of a controlling element. Science 209:1370–1374
    [Google Scholar]
  23. Slutsky V., Buffo J., Soll D.R. 1985; High-frequency switching of colony morphology in Candida albicans . Science 230:666–669
    [Google Scholar]
  24. Slutsky B., Staebell M., Anderson J., Risen L., Pfaller M., Soll D.R. 1987; “White-opaque transition”: a second high-frequency switching system in Candida albicans . Journal of Bacteriology 169:189–197
    [Google Scholar]
  25. Suzuki S. 1981; Antigenic determinants. In Yeast Cell Envelopes: Biochemistry, Biophysics, and Ultrastructure, 1 pp 85–96 Arnold W. N. Edited by Boca Raton, Florida: CRC Press;
    [Google Scholar]
  26. Suzuki T., Rogers A.L., Magee P.T. 1986a; Inter- and intra-species crosses between Candida albicans and Candida guilliermondii . Yeast 2:53–58
    [Google Scholar]
  27. Suzuki T., Kanbe T., Kuroiwa T., Tanaka K. 1986b; Occurrence of ploidy shift in a strain of the imperfect yeast Candida albicans . Journal of General Microbiology 132:443–453
    [Google Scholar]
  28. Whelan W.L., Magee P.T. 1981; Natural heterozygosity in Candida albicans . Journal of Bacteriology 145:896–903
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-2-425
Loading
/content/journal/micro/10.1099/00221287-135-2-425
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error