%0 Journal Article %A rai, A. N. %A borthakur, M. %A singh, S. %A bergman, B. %T Anthoceros-Nostoc Symbiosis: Immunoelectronmicroscopic Localization of Nitrogenase, Glutamine Synthetase, Phycoerythrin and Ribulose-1,5-bisphosphate Carboxylase/Oxygenase in the Cyanobiont and the Cultured (Free-living) Isolate Nostoc 7801 %D 1989 %J Microbiology, %V 135 %N 2 %P 385-395 %@ 1465-2080 %R https://doi.org/10.1099/00221287-135-2-385 %I Microbiology Society, %X Localization of nitrogenase, glutamine synthetase (GS), phycoerythrin (PE) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) was studied with immunocytochemical techniques in the cyanobiont and the free-living cultured isolate Nostoc 7801 of Anthoceros punctatus. In both cases nitrogenase was located in heterocysts only and was uniformly distributed within the cell. GS was located both in heterocysts and vegetative cells, with a uniform cellular distribution in each cell type. Whereas heterocysts of Nostoc 7801 had about twofold higher label than vegetative cells, labelling in heterocysts and vegetative cells of the cyanobiont was similar. While the GS content of the vegetative cells of the cyanobiont and Nostoc 7801 was comparable, the apparent GS content of the cyanobiont heterocysts was 60% less than that in Nostoc 7801 heterocysts. PE and RuBisCO were located in vegetative cells only. PE was located on thylakoid membranes and RuBisCO in the cytoplasm and carboxysomes. In each case the pattern of labelling in the cyanobiont and Nostoc 7801 was similar. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-2-385