1887

Abstract

Localization of nitrogenase, glutamine synthetase (GS), phycoerythrin (PE) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) was studied with immunocytochemical techniques in the cyanobiont and the free-living cultured isolate 7801 of . In both cases nitrogenase was located in heterocysts only and was uniformly distributed within the cell. GS was located both in heterocysts and vegetative cells, with a uniform cellular distribution in each cell type. Whereas heterocysts of 7801 had about twofold higher label than vegetative cells, labelling in heterocysts and vegetative cells of the cyanobiont was similar. While the GS content of the vegetative cells of the cyanobiont and 7801 was comparable, the apparent GS content of the cyanobiont heterocysts was 60% less than that in 7801 heterocysts. PE and RuBisCO were located in vegetative cells only. PE was located on thylakoid membranes and RuBisCO in the cytoplasm and carboxysomes. In each case the pattern of labelling in the cyanobiont and 7801 was similar.

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1989-02-01
2022-06-29
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References

  1. Bergman B., Lindblad P., Pettersson A., RenströM E., Tiberg E. 1985; Immunogold localization of glutamine synthetase in a nitrogenfixing cyanobacterium,Anabaena cylindrica . Planta 166:329–334
    [Google Scholar]
  2. Bergman B., Lindblad P., Rai A.N. 1986; Nitrogenase in free-living and symbiotic cyanobacteria: immunoelectronmicroscopic localization. FEMS Microbiology Letters 35:75–78
    [Google Scholar]
  3. Codd G.A., Marsden W.J.N. 1984; The carboxysomes (polyhedral bodies) of autotrophic prokaryotes. Biological Reviews 59:389–422
    [Google Scholar]
  4. Codd G.A., Okabe K., Stewart W.D.P. 1980; Cellular compartmentation of photosynthetic and photorespiratory enzymes in the heterocystous cyanobacteriumAnabaena cylindrica . Archives of Microbiology 124:149–154
    [Google Scholar]
  5. Cohen-Bazire G., Bryant D.A. 1982; Phycobili- somes: composition and structure. In The Biology of Cyanobacteria pp 143–190 Carr N. G., Whitton B. A. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  6. Cossar J.D., Rowell P., Darling A.J., Murray S., Codd G.A., Stewart W.D.P. 1985; Localization of ribulose 1,5-bisphosphate carboxy- lase/oxygenase in the N2-fixing cyanobacteriumAnabaena cylindrica . FEMS Microbiology Letters 28:65–68
    [Google Scholar]
  7. Englund B. 1977; The physiology of the lichenPeltigera aphthosa, with special reference to the blue- green phycobiont (Nostoc sp.). Physiologia plantarum 41:298–304
    [Google Scholar]
  8. Flemming H., Haselkorn R. 1974; The program of protein synthesis during heterocyst differentiation in nitrogen fixing blue-green algae. Cell 3:159–170
    [Google Scholar]
  9. Gallon J.R., Chaplin J.E. 1987 An Introduction to Nitrogen Fixation pp. 52–65 London:: Cassell Educational Limited.;
    [Google Scholar]
  10. Guard-Friar D., Eisenberg B.L., Edwards M.R., MacColl R. 1986; Immunocytochemistry of cryptomonad biliproteins. Plant Physiology 80:38–42
    [Google Scholar]
  11. Haällbom L., Bergman B., Rai A.N. 1986; Immunogold localization of glutamine synthetase in the cyanobiont of the lichensPeltigera aphthosa and Peltigera canina . Lichen Physiology and Biochemistry 1:27–34
    [Google Scholar]
  12. Ho K.K., Krogman D.W. 1982; Photosynthesis. In The Biology of Cyanobacteria pp 191–214 Carr N. G., Whitton B. A. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  13. Janaki S., Wolk C.P. 1982; Synthesis of nitrogenase in isolated heterocysts. Biochimica et biophysica acta 696:187–192
    [Google Scholar]
  14. Joseph C.M., Meeks J.C. 1987; Regulation of expression of glutamine synthetase in a symbioticNostoc strain associated with Anthoceros punctatus . Journal of Bacteriology 169:2471–2475
    [Google Scholar]
  15. Lindblad P. 1987 Nostoc-cycad symbiosis : with emphasis on the cyanobiont pp. 25–26 PhD thesis; University of Uppsala.:
    [Google Scholar]
  16. Lindblad P., Bergman B. 1986; Glutamine synthetase: activity and localization in cyanobacteria of the cycadsCycas revoluta and Zamia skinneri . Planta 169:1–7
    [Google Scholar]
  17. Lindblad P., Hällbom L., Bergman B. 1985; The cyanobacterium-Zamia symbiosis: C2H2 reduction and heterocyst frequency. Symbiosis 1:19–28
    [Google Scholar]
  18. Lindblad P., Rai A. N., Bergman B. 1987; Cycas revoluta-Nostoc symbiosis : enzyme activities of nitrogen and carbon metabolism in the cyanobiont. Journal of General Microbiology 133:1695–1699
    [Google Scholar]
  19. Meeks J.C., Enderlin C.S., Joseph C.M., Chapman J.S., Lollar M.W.L. 1985; Fixation of [13N]N2 and transfer of fixed nitrogen in theAnthoceros-Nostoc symbiotic association. Planta 164:406–414
    [Google Scholar]
  20. Murry M.A., Hallenbeck P.C., Benemann J.R. 1984; Immunochemical evidence that nitrogenase is restricted to heterocysts inAnabaena cylindrica . Archives of Microbiology 137:194–199
    [Google Scholar]
  21. Nierzwicki-Bauer S.A., Haselkorn R. 1986; Difference in mRNA levels inAnabaena living freely or in symbiotic association with Azolla . EMBO Journal 5:29–35
    [Google Scholar]
  22. Oelmüller R., Mohr H. 1986; Photooxidative destruction of chloroplasts and its consequences for expression of nuclear genes. Planta 167:106–133
    [Google Scholar]
  23. Orr J., Haselkorn R. 1982; Regulation of glutamine synthetase activity and synthesis in free- living and symbioticAnabaena spp. Journal of Bacteriology 152:626–635
    [Google Scholar]
  24. Rippka R., Stanier R.Y. 1978; The effects of anaerobiosis on nitrogenase synthesis and heterocyst differentiation by Nostocacean cyanobacteria. Journal of General Microbiology 105:83–94
    [Google Scholar]
  25. Rippka R., Waterbury J.B. 1977; The synthesis of nitrogenase by nonheterocystous cyanobacteria. FEMS Microbiology Letters 2:83–86
    [Google Scholar]
  26. Rippka R., Deruelles J., Waterbury J.B., Herdman M., Stanier R.Y. 1979; Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111:1–61
    [Google Scholar]
  27. Rodgers G.A., Stewart W.D.P. 1977; The cyanophyte-hepatic symbiosis. I. Morphology and physiology. New Phytologist 78:441–458
    [Google Scholar]
  28. Rowell P., Rai A.N., Stewart W.D.P. 1985; Studies on the nitrogen metabolism of the lichensPeltigera aphthosa and Peltigera canina . In Lichen Physiology and Cell Biology pp 145–160 Brown D. H. Edited by New York: Plenum Press;
    [Google Scholar]
  29. Smith D. C., Douglas A. E. 1987 The Biology of Symbiosis pp. 93–145 London:: Edward Arnold.;
    [Google Scholar]
  30. Spence D.W., Stewart W.D.P. 1987; Heterocystless mutants ofAnabaena 7120 with nitrogenase activity. FEMS Microbiology Letters 40:119–122
    [Google Scholar]
  31. Stal L.J., Krumbein W.E. 1985; Oxygen protection of nitrogenase in the aerobically nitrogen fixing, nonheterocystous cyanobacteriumOscilla- toria sp. Archives of Microbiology 143:72–76
    [Google Scholar]
  32. Stewart W.D.P. 1980; Some aspects of structure and function in N2-fixing cyanobacteria. Annual Review of Microbiology 34:497–536
    [Google Scholar]
  33. Stewart W.D.P., Lex M. 1970; Nitrogenase activity in the blue-green algaPlectonema boryanumstrain 594. Archiv für Mikrobiologie 73:250–260
    [Google Scholar]
  34. Stewart W.D.P., Rodgers G.A. 1977; The cyanophyte-hepatic symbiosis. II. Nitrogen fixation and the interchange of nitrogen and carbon. New Phytologist 78:459–471
    [Google Scholar]
  35. Stewart W.D.P., Rowell P., Rai A.N. 1983; Cyanobacteria-eukaryotic plant symbioses. Annates de microbiologie 134B:205–228
    [Google Scholar]
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