Surface washings were prepared from cultures of fungi grown on agar slants and the wash components were fractionated by gel-filtration HPLC. A range of low-Mr glycoproteins (< 10000) were detected in all the washes, and in some species several high-Mr glycoprotein components (> 150000) were also present. The HPLC analyses indicated species-specific different in the profiles. Regions of specific antigenicity within profiles were detected by monoclonal antibodies.
CooperR. M.1983; The mechanisms and significance of enzymic degradation of host cell walls by parasites. In Biochemical Plant Pathology pp. 101–135CallowJ. A.
Edited by New York: John Wiley;
DeweyF. M.,
MacdonaldM. M.,
PhillipsS. I.1989a; Development of monoclonal-antibody- ELISA, -DOT-BLOT and -DIP-STICK immunoassays for Humicola lanuginosa in rice. Journal of General Microbiology 135:361–364
DeweyF. M.,
MundayC. J.,
BrasierC. M.1989b; Monoclonal antibodies to specific components of the Dutch Elm disease pathogen Ophiostoma ulmi
. Plant Pathology in the Press
EvansG. H.,
CookeR. C.1982; Studies on Mucoralean mycoparasites. III. Diifusable factors from Mortierella vinacea Dixon-Stuart that direct germ tube growth in Piptocephalis fimbriata Richardson and Leadbeater. New Phytologist 91:245–253
FoungS. K. H.,
SasakiD. T.,
GrumetF. C.,
EnglemanE. C.1982; Production of functional human T-T hybridomas in selection medium lacking aminopterin and thymidine. Proceedings of the National Academy of Sciences of the United States of America 79:7484–7488
KennettR. H.,
DenisK. A.,
TungA. S.,
KlinmanN. R.1978; Hybrid plasmacytoma production: fusion with adult spleen cells, monoclonal spleen fragments, neotonal spleen cells and human spleen cells. Current Topics in Microbiology and Immunology 81:77–91
TaylorI. E. P.,
CameronD. S.1973; Preparation and quantitative analysis of fungal cell walls: strategy and tactics. Annual Review of Microbiology 27:243–261