1887

Abstract

The A2 substance, a structural component of the cell wall of alkalophilic strain C-125, has an of 21000 and is composed mainly of glucuronic acid and glutamic acid. Hydrazinolysis of the A2 substance yielded homoglucuronate with an estimated of 4800. The of polyglutamate previously prepared from the A2 substance by deglycosylation was estimated to be 14000. It is therefore suggested that the A2 substance is composed of the two polymers, polyglutamate and polyglucuronate, and the name ‘teichuronopeptide’ is proposed for this complex.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-2-265
1989-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/2/mic-135-2-265.html?itemId=/content/journal/micro/10.1099/00221287-135-2-265&mimeType=html&fmt=ahah

References

  1. Aono R. 1985; Isolation and partial characterization of structural components of the walls of alkalophilic Bacillus strain C-125. Journal of General Microbiology 131:105–111
    [Google Scholar]
  2. Aono R. 1987; Characterization of structural component of cell walls of alkalophilic strain of Bacillus sp. C-125. Biochemical Journal 245:467–472
    [Google Scholar]
  3. Aono R., Horikoshi K. 1983; Chemical composition of cell walls of alkalophilic strains of Bacillus . Journal of General Microbiology 129:1083–1087
    [Google Scholar]
  4. Aono R., Uramoto M. 1986; Presence of fucosamine in teichuronic acid of the alkalophilic Bacillusstrain C-125. Biochemical Journal 233:291–294
    [Google Scholar]
  5. Bartnicki-Garcia S., Reyes E. 1968; Polyuronides in the cell walls of Mucor rouxii . Biochimica et biophysica acta 170:54–62
    [Google Scholar]
  6. Beutler H.O., Michal G. 1974; Glutamate, determination with glutamate dehydrogenase, diaphorase and tetrazolium salts. In Methods of Enzymatic Analysis, 4 pp 1708–1713 Bergmeyer H. U. Edited by Weinheim: Verlag Chemie;
    [Google Scholar]
  7. Carlson D.M., Matthews L.W. 1966; Polyuronic acids produced by Pseudomonas aeruginosa . Biochemistry 5:2817–2822
    [Google Scholar]
  8. Gorin P.A.J., Spencer J.F.T. 1966; Exocellular alginic acid from Azotobacter vinelandii . Canadian Journal of Chemistry 44:993–1001
    [Google Scholar]
  9. Haug A., Larsen B., Smidsrød O. 1966; A study of the constitution of alginic acid by partial acid hydrolysis. Acta chemica scandinavica 20:183–190
    [Google Scholar]
  10. Järnefelt J., Rush J.S., Ashraf J., Viitala J., Laine A. 1982; Preparation of high molecular weight glycopeptides and oligosaccharides (erythroglycan) from human erythrocytes. Methods in Enzymology 83:311–320
    [Google Scholar]
  11. Linker A., Jones R.S. 1966; A new polysaccharide resembling alginic acid isolated from Pseudomonas . Journal of Biological Chemistry 241:3845–3851
    [Google Scholar]
  12. Skjak-Braek G., Grasdalen H., Larsen B. 1986; Monomer sequence and acetylation pattern in some bacterial alginates. Carbohydrate Research 154:239–250
    [Google Scholar]
  13. Spiro R.G. 1966; Characterization of carbohydrate units of glycoproteins. Methods in Enzymology 8:311–320
    [Google Scholar]
  14. Takasaki S., Mizouchi T., Kobata A. 1982; Hydrazinolysis of asparagine-linked sugar chains to produce free oligosaccharides. Methods in Enzymology 83:263–268
    [Google Scholar]
  15. Taylor R.L., Conrad H.E. 1972; Stoichiometric depolymerization of polyuronides and glycosaminoglycans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups. Biochemistry 11:1383–1388
    [Google Scholar]
  16. Tsuchihashi T., Yadomae T., Miyazaki T. 1983; Structural analysis of the cell-wall d-glucuronans from the fungi Absidia cylindrospora, Mucor mucedo and Rhizopus nigricans . Carbohydrate Research 111:330–335
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-2-265
Loading
/content/journal/micro/10.1099/00221287-135-2-265
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error