1887

Abstract

Iron reduction and uptake was studied in wild-type and haem-deficient strains of . Haem-deficient strains lacked inducible ferri-reductase activity and were unable to take up iron from different ferric chelates such as Fe(III)-citrate or rhodotorulic acid. In contrast, ferrioxamine B was taken up actively by the mutants as well as by the wild-type strains. At a low extracellular concentration, uptake was insensitive to ferrozine and competitively-inhibited by Ga(III)-desferrioxamine B. Extracellular reductive dissociation of the siderophore occurred at higher extracellular concentrations. Two mechanisms appear to contribute to the uptake of ferrioxamine B by : one with high affinity, by which the siderophore is internalized as such and another with lower affinity by which iron is dissociated from the ligand prior to uptake.

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1989-02-01
2021-09-24
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References

  1. Bagg A. , Neilands J.B. . 1987; Molecular mechanism of siderophore-mediated iron assimilation. Microbiological Reviews 51:509–518
    [Google Scholar]
  2. Bienfait F. . 1985; Regulated redox processes at the plasmalemma of plant root cells and their function in iron uptake. Journal of Bioenergetics and Biomembranes 17:73–83
    [Google Scholar]
  3. Bienfait F. . 1987; Biochemical basis of iron efficiency reactions in plants. In Iron Transport in Microbes, Plants and Animals pp. 339–352 Winkelmann G. , Van der Helm D. , Neilands J. B. . Edited by Weinheim & New York: VCH;
    [Google Scholar]
  4. Camadro J.-M. , Urban-Grimal D. , Labbe P. . 1982; A new assay for protoporphyrinogen oxidase - evidence for total deficiency in that activity in a heme-less mutant of Saccharomyces cerevisiae . Biochemical and Biophysical Research Communications 106:724–730
    [Google Scholar]
  5. Castignetti D. , Smarrelli J. . 1986; Siderophores, the iron nutrition of plants, and nitrate reductase. FEBS Letters 209:147–151
    [Google Scholar]
  6. Crowley D.E. , Reid C.P.P. , Szaniszlo P.J. . 1987; Microbial siderophores as iron sources for plants. In Iron Transport in Microbes, Plants and Animals pp. 375–386 Winkelmann G. , Van der Helm D. , Neilands J. B. . Edited by Weinheim & New York: VCH;
    [Google Scholar]
  7. Ecker D.J. , Emery T. . 1983; Iron uptake from ferrichrome A and iron citrate in Ustilago sphaerogena . Journal of Bacteriology 155:616–622
    [Google Scholar]
  8. Emery T. . 1971; Role of ferrichrome as a ferric ionophore in Ustilago sphaerogena . Biochemistry 10:1483–1488
    [Google Scholar]
  9. Emery T. . 1983; Reductive mechanism for fungal iron transport. In Microbiology-1983. Schlessinger D. . Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Emery T. . 1986; Exchange of iron by gallium in siderophores. Biochemistry 25:4629–4633
    [Google Scholar]
  11. Emery T. . 1987; Reductive mechanisms of iron assimilation. In Iron Transport in Microbes, Plants and Animals pp. 235–250 Winkelmann G. , Van der Helm D. , Neilands J. B. . Edited by Weinheim & New York: VCH;
    [Google Scholar]
  12. Emery T. , Hoffer P.B. . 1980; Siderophore- mediated mechanism of gallium uptake demonstrated in the microorganism Ustilago sphaerogena . Journal of Nuclear Medicine 21:935–939
    [Google Scholar]
  13. Lesuisse E. , Raguzzi F. , Crichton R.R. . 1987; Iron uptake by the yeast Saccharomyces cerevisiae: involvement of a reduction step. Journal of General Microbiology 133:3229–3236
    [Google Scholar]
  14. Lewis T.A. , Taylor F.R. , Parks L.W. . 1985; Involvement of heme biosynthesis in control of sterol uptake by Saccharomyces cerevisiae . Journal of Bacteriology 163:199–207
    [Google Scholar]
  15. Neilands J.B. , Konopka K. , Schwyn B. , Coy M. , Francis R.T. , Paw B.H. , Bagg A. . 1987; Comparative biochemistry of microbial iron assimilation. In Iron Transport in Microbes, Plants and Animals pp. 3–34 Winkelmann G., Van der Helm D., Neilands J. B. Edited by Weinheim & New York: VCH;
    [Google Scholar]
  16. Nicholas D.J.D. 1957; Microbiological methods for determining magnesium, iron, copper, zinc, manganese and molybdenum. Methods in Enzymology 3:1035–1041
    [Google Scholar]
  17. Raguzzi F., Lesuisse E., Crichton R.R. 1988; Iron storage in Saccharomyces cerevisiae . FEBS Utters 231:253–258
    [Google Scholar]
  18. Romheld V. 1987; Existence of two strategies for the acquisition of iron in higher plants. In Iron Transport in Microbes, Plants and Animals pp. 353–374 Winkelmann G., Van der Helm D., Neilands J. B. Edited by Weinheim & New York: VCH;
    [Google Scholar]
  19. Schwyn B., Neilands J.B. 1987; Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry 160:47–56
    [Google Scholar]
  20. Urban-Grimal D., Labbe-Bois R. 1981; Genetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis. Molecular and General Genetics 183:85–92
    [Google Scholar]
  21. Winkelmann G., Huschka H.-G. 1987; Molecular recognition and transport of siderophores in fungi. In Iron Transport in Microbes, Plants and Animals pp. 317–336 Winkelmann G., Van der Helm D., Neilands J. B. Edited by Weinheim & New York: VCH;
    [Google Scholar]
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