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Volume 135, Issue 12

Enhancement of Bacteriophage φX-174 Plaques by Homoionic Clay Minerals Free

Abstract

The incorporation of selected particulates or charged polymers into qualitative and quantitative assay systems has been found to increase virus specific infectivity. The purpose of this study was to identify a mechanism(s) to explain the effect of charged particulates upon plaquing efficiency. The clay minerals kaolinite (K), montmorillonite (M), with the bacteriophage øX-174 and its host , were used as a model system. Enhanced bacteriophage infectivity (expressed as plaque-forming units) occurred at K and M concentrations in the agar overlay of 0·14–0·0 and 0·01·15 mg ml, respectively. A maximum increase of bacteriophage titre (to 180–230% of the control value) occurred at 0·18–0·20 and 0·06–0·08 mg mlK and M, respectively. Increased infectivity was related to the type of clay mineral and to the cation saturating the exchange complex in the order K homoionic (i.e. saturated with a single cation type) to Na> Mg> Kand M homoionic to Mg> Na> K. Enhanced infectivity of the bacteriophage was not related to the surface area of K nor to the external or total surface area of M. The increase of bacteriophage titre above that of the control was only minimally related to cation-exchange capacity, but was associated with the anion-exchange capacity of the clay. The increased plaquing efficiency is ascribed to the formation of high-density bacteria-clay microcosms which, in turn, modify bacteriophage contact and adsorption to the host cell.

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© Society for General Microbiology 1989
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1989-12-01
2023-03-21
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References

  1. Adams A. M. 1959; Methods of study of bacterial viruses.. In The Bacteriophages pp. 443–457 Adams A. M. Edited by New York: Wiley;
     [Google Scholar]
  2. Armon R., Cabelli V. J. 1988; Phage f2 desorption from clays in estuarine water using nonionic detergents, beef extract, and chaotropic agents.. Canadian Journal of Microbiology 34:1022–1024
     [Google Scholar]
  3. Bishai F. R., Labzoffsky N. A. 1974; Stability of different viruses in a newly developed transport medium.. Canadian Journal of Microbiology 20:75–81
     [Google Scholar]
  4. Boyce D. S., Sproul O. J., Buck C. E. 1981; The effect of bentonite clay on ozone disinfection of bacteria and viruses in water.. Water Research 15:759–767
     [Google Scholar]
  5. Brock T. D. 1966 Microbial Ecology pp. 16–22 Englewood Cliffs, NJ: Prentice-Hall;
     [Google Scholar]
  6. Burge W. D., Enkiri N. K. 1978; Virus adsorption by five soils.. Journal of Environmental Quality 1:73–76
     [Google Scholar]
  7. Chaikum N., Sooppipatt N., Carr R. M. 1981; The cation exchange capacity of some kaolin minerals.. Journal of the Science Society of Thailand 7:100–109
     [Google Scholar]
  8. Craighead J. E., Lane C. H. 1969; Contrasting effects of polycations of plaquing efficiency of encephalomyocarditis virus variants.. Journal of Virology 3:45–51
     [Google Scholar]
  9. Crandell R. A., Gomes I. 1970; Plaque morphology of some South American strains of foot-and- mouth disease virus and the effect of polyionic compounds on plaque formation.. Archiv für die gesamte Virusforschung 30:137–146
     [Google Scholar]
  10. Drewry W. A., Eliassen R. E. 1968; Virus movement in groundwater.. Journal of the Water Pollution Control Federation 40:257–271
     [Google Scholar]
  11. Dunn D. B., Hitchborne J. H. 1965; The use of bentonite in the purification of plant viruses.. Virology 25:171–192
     [Google Scholar]
  12. Ferris A. P., Jepson W. B. 1975; The exchange capacities of kaolinite and the preparation of homoionic clays.. Journal of Colloid and Interface Science 51:245–259
     [Google Scholar]
  13. Goyal S. M., Gerba C. P. 1979; Comparative adsorption of human enteroviruses, simian rotavirus and selected bacteriophages to soils.. Applied and Environmental Microbiology 38:241–247
     [Google Scholar]
  14. Goyal S. M., Zerda K. S., Gerba C. P. 1980; Concentration of coliphages from large volumes of water and wastewater.. Applied and Environmental Microbiology 39:85–91
     [Google Scholar]
  15. Grim R. E. 1968 Clay Mineralogy, 2nd edn.. pp. 189226 New York: McGraw-Hill;
     [Google Scholar]
  16. Kaplan M. M., Kiktor T. J., Maes R. F., Campbell J. B., Koprowski H. 1967; Effect of polyions on the infectivity of rabies virus in tissue culture: construction of a single-cycle curve.. Journal of Virology 1:145–151
     [Google Scholar]
  17. Koya K.V.A., Chaudhuri M. 1977; Virus retention by soil.. Progress in Water Technology 9:43–52
     [Google Scholar]
  18. Lipson S. M., Stotzky G. 1983; Adsorption of reovirus to clay minerals: effects of cation exchange capacity, cation saturation, and surface area.. Applied and Environmental Microbiology 46:673–682
     [Google Scholar]
  19. Lipson S. M., Stotzky G. 1984; Effect of proteins on reovirus adsorption to clay minerals.. Applied and Environmental Microbiology 48:525–530
     [Google Scholar]
  20. Lipson S. M., Stotzky G. 1985; Infectivity of reovirus adsorbed to homoionic and mixed-cation clays.. Water Research 19:227–234
     [Google Scholar]
  21. Lipson S. M., Stotzky G. 1987; Interactions between clay minerals and viruses.. In Human Viruses in Sediments, Sludges, and Soils pp. 187–230 Rao V. C., Melnick J. L. Edited by Boca Raton, Florida: CRC Press;
     [Google Scholar]
  22. Marshall K. C. 1968; Interactions between colloidal montmorillonite and cells of Rhizobium species with different ionogenic surfaces.. Biochimica et biophysica acta 156:179–186
     [Google Scholar]
  23. Marshall K. C. 1969a; Studies by microelectrophoresis and microscope techniques of the adsorption of illite and montmorillonite to rhizobia.. Journal of General Microbiology 56:301–306
     [Google Scholar]
  24. Marshall K. C. 1969b; Orientation of clay particles on bacteria possessing different ionogenic surfaces.. Biochimica et biophysica acta 193:472–474
     [Google Scholar]
  25. Marshall K. C. 1975; Clay mineralogy in relation to survival of soil bacteria.. Annual Review of Phytopathology 13:357–373
     [Google Scholar]
  26. Moore B. E., Sagik B. P., Malina J. F. Jr 1975; Viral association with suspended solids.. Water Research 9:197–203
     [Google Scholar]
  27. Nguyen T. D., Bottreau E., Aynaud J. M. 1987; Transmissible gastroenteritis (TGE) of swine: in vitro virus attachment and effects of polyanions and polycations.. Veterinary Microbiology 14:343–354
     [Google Scholar]
  28. Roper M. M., Marshall K. C. 1974; Modification of the interaction between Escherichia coli and bacteriophage in saline sediment.. Microbial Ecology 1:1–13
     [Google Scholar]
  29. Roper M. M., Marshall K. C. 1978a; Effect of clay particle size on clay-Escherichia co/z-bacterio- phage interactions.. Journal of General Microbiology 106:187–189
     [Google Scholar]
  30. Roper M. M., Marshall K. C. 1978b; Effects of clay minerals on microbial predation and parasitism of Escherichia coli. . Microbial Ecology 4:279–289
     [Google Scholar]
  31. Santoro T., Stotzky G. 1967a; Effect of electrolyte composition and pH on the particle size distribution of microorganisms and clay minerals as determined by the electrical sensing zone method.. Archives of Biochemistry and Biophysics 122:664–669
     [Google Scholar]
  32. Santoro T., Stotzky G. 1967b; Influence of cations on flocculation of clay minerals by microbial metabolites as determined by the electrical zone particle analyzer.. Soil Science Society of America Proceedings 31:761–765
     [Google Scholar]
  33. Santoro T., Stotzky G. 1968; Sorption between microorganisms and clay minerals as determined by the electrical sensing zone particle analyzer.. Canadian Journal of Microbiology 14:299–309
     [Google Scholar]
  34. Schaub S. A., Kenyon K. F., Bledsoe B., Thomas R. E. 1980; Evaluation of the overland runoff mode of land wastewater treatment for virus removal.. Applied and Environmental Microbiology 39:127–134
     [Google Scholar]
  35. Shirobokov V. P. 1968; Differentiation of coxsackieviruses based on the character of adsorption onto bentonite.. Acta virologica 12:185
     [Google Scholar]
  36. Shirobokov V. P. 1973; Use of bentonite overlay for titration of enteroviruses by the plaque method.. Voprosy virusologii 18:611–615
     [Google Scholar]
  37. Sykes I. K., Williams S. T. 1978; Interactions of actinophages and clays.. Journal of General Microbiology 108:97–102
     [Google Scholar]
  38. Takemoto K. K., Liebhaber H. 1962; Virus- polysaccharide interactions. II. Enhancement of plaque formation and detection of variants of poliovirus with dextran sulfate.. Virology 17:499–501
     [Google Scholar]
  39. Toyoshima K., Vogt P. K. 1969; Enhancement and inhibition of avian sarcoma virus by polycations and polyanions.. Virology 38:414–426
     [Google Scholar]
  40. Vaheri A., Sedwick W. D., Plotkin S. A. 1967; Growth of rubella virus in BHK 21 cells. II. Enhancing effect of DEAE-dextran, semicarbazide and low doses of metabolic inhibitors.. Proceedings of the Society for Experimental Biology and Medicine 125:1092–1098
     [Google Scholar]
  41. Wallis C., Melnick J. L. 1965; Differences in cysteine dependence and chromatographic behavior between two type 4 echovirus strains.. Journal of Bacteriology 89:1310–1313
     [Google Scholar]
  42. Wallis C., Melnick J. L. 1968; Mechanism of enhancement of virus plaques by cationic polymers.. Journal of Virology 2:267–274
     [Google Scholar]
  43. Wiggins B. A., Alexander M. 1985; Minimum bacterial density for bacteriophage replication: implications for significance of bacteriophages in natural ecosystems.. Applied and Environmental Microbiology 49:19–23
     [Google Scholar]
  44. Wudunn D., Spear P. C. 1989; Initial interaction of herpes simplex with cells is binding to heparin sulfate.. Journal of Virology 63:52–58
     [Google Scholar]
  45. Wunner W. H., Reagan K. J., Kaprowski H. 1984; Characterization of saturable binding sites for rabies virus.. Journal of Virology 50:691–697
     [Google Scholar]
  46. Yarwood C. E. 1966; Bentonite aids virus transmission.. Virology 28:459–462
     [Google Scholar]
  47. Yarwood C. E. 1972; Virus transmission from Chenopodium amaranticolor. . Plant Disease Reporter 56:1085–1086
     [Google Scholar]
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Enhancement of Bacteriophage φX-174 Plaques by Homoionic Clay Minerals
Microbiology 135, 3497 (1989); https://doi.org/10.1099/00221287-135-12-3497
/content/journal/micro/10.1099/00221287-135-12-3497
/content/journal/micro/10.1099/00221287-135-12-3497
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