1887

Abstract

The growth of the dematiaceous hyphomycetes (darkly pigmented microfungi) and was altered by increasing concentrations of NaCl in the growth medium. demonstrated the greatest sensitivity to salinity changes: chlamydospora-like structures were common at NaCl concentrations > 5 %. Lipid analyses of these fungi are presented. Steryl esters and fatty acids were the main lipid classes in the apolar fractions. Phosphatidylethanolamine, phosphatidylgly-cerol and phosphatidylcholine were the main polar compounds. Most phospholipids decreased at higher salinity. The major fatty acid was oleic acid (18:1) which decreased at 10 % NaCl. A similar pattern was observed for palmitic acid (16:0). Myristic acid (14:0) and myristoleic acids (14:2, 14:1) were detected in all isolates cultured at 10 % NaCl.

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1989-12-01
2022-01-20
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References

  1. Adams R., Bygraves J., Kogut M., Russell N. J. 1987; The role of osmotic effects in haloadaptation of Vibrio costicola.. Journal of General Microbiology 133:1861–1870
    [Google Scholar]
  2. Al-Hasan R. H., Ghannoum M. A., Sallal A. K., Abu-Elteen K. H., Radwan S. S. 1987; Correlative changes of growth, pigmentation and lipid composition of Dunaliella salina in response to halostress.. Journal of General Microbiology 133:2607–2616
    [Google Scholar]
  3. Avron M., Ben-Amotz AW. 1979; Metabolic adaptation of the alga Dunaliella to low water activity.. In Strategies of Microbial Life in Extreme Environments pp. 83–91 Shilo M. Edited by Berlin: Dahlem Konferenzen;
    [Google Scholar]
  4. Brown A. D. 1972; Microbial water relations: features of the intracellular composition of sugar- tolerant yeasts.. Journal of Bacteriology 118:769–777
    [Google Scholar]
  5. Brown A. D. 1976; Microbial water stress.. Bacteriology Reviews 40:803–846
    [Google Scholar]
  6. Chalvardjian V. 1964; Fatty acids of brown and yellow fat in rats.. Biochemical Journal 90:518–521
    [Google Scholar]
  7. Cole G. T. 1986; Preparation of microfungi for scanning electron microscopy.. In Ultrastructure Techniques for Micro-organisms pp. 1–38 Aldrich H. C., Todd W. J. Edited by New York: Plenum Press;
    [Google Scholar]
  8. Combs T. J., Guarneri J. J., Pisano M. A. 1968; The effect of sodium chloride on the lipid content and fatty acid composition of Candida albicans.. Mycologia 60:1232–1239
    [Google Scholar]
  9. Dittmer J. C., Lester R. L. 1964; A simple specific spray for the detection of phospholipids on thin layer chromatograms.. Journal of Lipid Research 5:126–127
    [Google Scholar]
  10. Folch J., Lees M., Sloane Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissue.. Journal of Biological Chemistry 226:497–509
    [Google Scholar]
  11. Grant W. D., Long P. E. 1981 Environmental Microbiology London: Blackie;
    [Google Scholar]
  12. Kushner D. J. 1978 Microbial Life in Extreme Environments New York: Academic Press;
    [Google Scholar]
  13. Mangold H. K., Malins D. C. 1960; Fractionation of fats, oils and waxes on thin layers of silicic acid.. Journal of the American Oil Chemists Society 37:383–385
    [Google Scholar]
  14. Miller K. J. 1985; Effects of temperature and sodium chloride concentration on the phospholipid and fatty acid composition of a halotolerant Piano- coccus sp.. Journal of Bacteriology 162:263–270
    [Google Scholar]
  15. Moustafa A. F. 1975; Osmophilous fungi in the salt marshes of Kuwait.. Canadian Journal of Microbiology 21:1573–1580
    [Google Scholar]
  16. Nichols B. W. 1964; Separation of plant phospholipids and glycolipids.. In New Biochemical Separations. James A. T., Morris I. J. Edited by London: Van Nostrand;
    [Google Scholar]
  17. Norris J. R., Ribbons D. W. 1970 Methods in Microbiology 3A:122
    [Google Scholar]
  18. Ohno Y., Yano I., Hiramatsu T., Masui M. 1976; Lipids and fatty acids of a moderately halophilic bacterium no. 101.. Biochimica et biophysica acta 424:337–350
    [Google Scholar]
  19. Ohno Y., Yano I., Masui M. 1979; Effect of NaCl concentration and temperature on the phospholipid and fatty acid composition of a moderately halophilic bacterium, Pseudomonas halosaccarolytica.. Journal of Biochemistry 85:413–421
    [Google Scholar]
  20. Paton F. M., Jennings D. H. 1988; Effect of sodium and potassium chloride and polyols on malate and glucose-6-phosphate dehydrogenases from the marine fungus Dendryphiella salina.. Transactions of the British Mycological Society 91:205–215
    [Google Scholar]
  21. Pugh G.J.F. 1962; Studies of fungi in coastal soils. II. Fungal ecology in a developing salt marsh.. Transactions of the British Mycological Society 45:560–566
    [Google Scholar]
  22. Radwan S. S., El-Essawy A. A., Helal G. A. 1984; Salinity loving fungi in Egyptian soils. I. Numbers, identification and halophilism.. Zentral- blatt fur Mikrobiologie 139:435–440
    [Google Scholar]
  23. Siakotos A. N., Rouser G. 1965; Analytical separation of non-lipid water soluble substances and gangliosides from other lipids by dextran gel column chromatography.. Journal of the American Oil Chemists Society 42:913–919
    [Google Scholar]
  24. Tresner H. D., Hayes I. A. 1971; Sodium chloride tolerance of terrestrial fungi.. Applied Microbiology 22:210–213
    [Google Scholar]
  25. Vioque E. 1984; Spray reagents for thin layer chromatography (TLC) and paper chromatography (PC).. In Handbook of Chromatography pp. 309–317 Mangold H. K. Edited by Boca Raton, Florida: CRC Press;
    [Google Scholar]
  26. Weete J. D. 1980 Lipid Biochemistry of Fungi and Other Organisms New York: Plenum Press;
    [Google Scholar]
  27. Wethered J. M., Metcalf E. C., Jennings D. H. 1985; Carbohydrate metabolism in the fungus Dendryphiella salina. VIII. The contribution of polyols and ions to the mycelial solute potential in relation to external osmoticum.. New Phytologist 101:631–649
    [Google Scholar]
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