1887

Abstract

A laboratory yeast isolate identified as synthesized melanin and showed polyphenol oxidase activity with dihydroxyphenylalanine (DOPA) and pyrogallol. Electrophoresis on native and denaturing gels, and activity staining with DOPA revealed two immunologically distinguishable bands of M about 100000 and 120000, which could be separated by (NH)SO fractionation. The enzyme isolated from this yeast gave optimal activity at higher pH (8·5–10) and temperature (37–40 °C) than other known melanin-synthesizing activities.

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/content/journal/micro/10.1099/00221287-135-12-3385
1989-12-01
2021-08-01
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References

  1. Barber J. H., Townsend D., Olds D. P., King R. A. 1984; Dopachrome oxidoreductase: a new enzyme in the pigment pathway.. Journal of Investigative Dermatology 83:145–149
    [Google Scholar]
  2. Broach J. R., Strathern J. N., Hicks J. B. 1979; Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.. Gene 8:121–123
    [Google Scholar]
  3. Burnett J. B. 1971; The tyrosinase of mouse melanoma. Isolation and molecular properties.. Journal of Biological Chemistry 246:3079–3089
    [Google Scholar]
  4. Dedeken R. H. 1966; The Crabtree effect: a regulatory system in yeast.. Journal of General Microbiology 44:149–156
    [Google Scholar]
  5. Katz E., Thompson J. C., Hopwood D. A. 1983; Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.. Journal of General Microbiology 129:2703–2714
    [Google Scholar]
  6. Keevil T., Mason H. S. 1978; Molecular oxygen in biological oxidations - an overview.. Methods in Enzymology 52:3–40
    [Google Scholar]
  7. Korner A., Pawalek J. M. 1982; Mammalian tyrosinase catalyses three reactions in the biosynthesis of melanin.. Science 217:1163–1165
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature; London: 111680–685
    [Google Scholar]
  9. Lehninger A. L. 1982; Enzymes: kinetics and inhibition.. In Principles of Biochemistry pp. 147–168 New York:: Worth Publishers.;
    [Google Scholar]
  10. Lerch K. 1978; Amino acid sequence of tyrosinase from Neurospora crassa.. Proceedings of the National Academy of Sciences of the United States of America 75:3635–3639
    [Google Scholar]
  11. Lerch K. 1981; Copper monooxygenases: tyrosinases and dopamine beta monooxygenase.. In Metal Ions in Biological Systems pp. 143–186 Sigel H. Edited by New York: Marcel Dekker;
    [Google Scholar]
  12. Nayak K. K., Padbidri A., Hirlekar M. G., Pandey N. K. 1986; Rapid method for preparation of protoplasts of Rhodotorula glutinis.. Current Science 55:926–927
    [Google Scholar]
  13. Nayak K. K., Pandey N. K., Roy P. 1988; Cloning of tyrosinase gene from Streptomyces livi-dans in Escherichia coli.. Indian Journal of Biochemistry and Biophysics 25:515–517
    [Google Scholar]
  14. Swan G. A. 1974; Structure, chemistry and biosynthesis of the melanins.. In Progress in the Chemistry of Organic Natural Products 31 pp. 521–582 Herz W., Grisebach H., Kirby G. W. Edited by New York: Springer Verlag;
    [Google Scholar]
  15. Tripathi R. K., Chaaya Devi C., Ramaiah A. 1988; pH dependent interconversion of two forms of tyrosinase in human skin.. Biochemical Journal 252:481–487
    [Google Scholar]
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