1887

Abstract

Summary: A laboratory yeast isolate identified as synthesized melanin and showed polyphenol oxidase activity with dihydroxyphenylalanine (DOPA) and pyrogallol. Electrophoresis on native and denaturing gels, and activity staining with DOPA revealed two immunologically distinguishable bands of M about 100000 and 120000, which could be separated by (NH)SO fractionation. The enzyme isolated from this yeast gave optimal activity at higher pH (8·5-10) and temperature (37-40°C) than other known melanin-synthesizing activities.

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/content/journal/micro/10.1099/00221287-135-12-3385
1989-12-01
2019-10-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-12-3385
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