1887

Abstract

Ca- and calmodulin-dependent protein kinase was purified from baker’s yeast to near homogeneity. At pH 7·5 and 0·1 -NaCl it has a native molecular mass close to 100 kDa, and is a dimer of apparently identical 56 kDa autophosphorylatable subunits. At 60 μ-CaCl and with mixed histones as substrate, half-maximal activation required concentrations of beef calmodulin above 1 μ. At 0·14 μ-beef calmodulin the enzyme showed apparent negative cooperativity towards ATP, with limiting apparent values of 4 μ and 60 μ ATP. The enzyme has a broad substrate specificity , including two yeast proteins that yield, respectively, 50 kDa and 200 kDa phosphopolypeptides.

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1989-12-01
2024-10-03
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References

  1. Andrews P. 1965; The gel-filtration behaviour of proteins related to their molecular weights over a wide range.. Biochemical Journal 96:595–606
    [Google Scholar]
  2. Arndt K. T., Styles C. A., Fink G. R. 1989; A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases.. Cell 56:527–537
    [Google Scholar]
  3. Breant B., Buhler J.-M., Sentenac A., Fromageot P. 1983; On the phosphorylation of yeast RNA polymerases A and B.. European Journal of Biochemistry 130:247–251
    [Google Scholar]
  4. Colbran R. J., Schworer C. M., Hashimoto Y., Fong Y.-L., Rich D. P., Smith M. K., Soderling T. R. 1989a; Calcium/calmodulin- dependent protein kinase II.. Biochemical Journal 258:313–325
    [Google Scholar]
  5. Colbran R. J., Smith M. K., Schworer C. M., Fong Y.-L., Soderling T. R. 1989b; Regulatory domain of calcium/calmodulin-dependent protein kinase II.. Journal of Biological Chemistry 264:4800–4804
    [Google Scholar]
  6. Cooper J. A., Sefton B. M., Hunter T. 1983; Detection and quantitation of phosphotyrosine in proteins.. Methods in Enzymology 99:387–402
    [Google Scholar]
  7. Davis T. N., Urdea M. S., Masiarz F. R., Thorner J. 1986; Isolation of the yeast calmodulin gene: calmodulin is an essential protein.. Cell 47:423–431
    [Google Scholar]
  8. Geahlen R. L., Anostario M.JR Low P. S., Harrison M. L. 1986; Detection of protein kinase activity in sodium dodecylsulfate-polyacrylamide gels.. Analytical Biochemistry 153:151–158
    [Google Scholar]
  9. Hixson C. S., Krebs E. G. 1980; Characterization of a cyclic AMP-binding protein from bakers’ yeast.. Journal of Biological Chemistry 255:2137–2145
    [Google Scholar]
  10. Huttner W. B., Schiebler W., Greengard P., De Camilli P. 1983; Synapsin1 (protein 1) a nerve terminal specific phosphoprotein.. Journal of Cellular Biology 96:1374–1388
    [Google Scholar]
  11. Kuznicki J. 1986; Phosphorylation of myosin in non-muscle and smooth muscle cells.. FEBS Letters 204:169–176
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
    [Google Scholar]
  13. Londesborough J. 1986; Phosphorylation of proteins in Clostridium thermohydrosulfuricum.. Journal of Bacteriology 165:595–601
    [Google Scholar]
  14. Londesborough J., Nuutinen M. 1987; Ca2+/ calmodulin-dependent protein kinase in Saccharomyces cerevisiae.. FEBS tetters 219:249–253
    [Google Scholar]
  15. Matsumoto K., Uno I., Ishikawa T. 1985; Genetic analysis of the role of cyclic AMP in yeast.. Yeast 1:15–24
    [Google Scholar]
  16. Miyakawa T., Oka Y., Tsuchiya E., Fukui S. 1989; Saccharomyces cerevisiae protein kinase dependent on Ca2+ and calmodulin.. Journal of Bacteriology 171:1417–1422
    [Google Scholar]
  17. Muthukumar G., Nickerson A. W., Nickerson K. W. 1987; Calmodulin levels in yeasts and filamentous fungi.. FEMS Microbiology tetters 41:253–255
    [Google Scholar]
  18. Nurse P. 1985; Yeast aids cancer research.. Nature, London 313:631–632
    [Google Scholar]
  19. Ohya Y., Ohsumi Y., Anraku Y. 1984; Genetic study of the role of calcium ions in the cell division cycle of Saccharomyces cerevisiae: a calcium-dependent mutant and its trifluoperazine-dependent pseudorevertants.. Molecular and General Genetics 193:389–394
    [Google Scholar]
  20. Ohya Y., Uno I., Ishikawa T., Anraku Y. 1987; Purification and biochemical properties of calmodulin from Saccharomyces cerevisiae.. European Journal of Biochemistry 168:13–19
    [Google Scholar]
  21. Pausch M. H., Thorner J. 1989; A Ca2+/ calmodulin-dependent, multifunctional protein kinase activity from yeast.. Journal of Cellular Biochemistry Supplement 13E p. 31
    [Google Scholar]
  22. Payne M. E., Schworer C. M., Soderling T. R. 1983; Purification and characterization of rabbit liver calmodulin-dependent glycogen synthase kinase.. Journal of Biological Chemistry 258:2376–2382
    [Google Scholar]
  23. Saavedra-Molina A., Villalobos R., Borbolla M. 1983; Calcium uptake during the cell cycle of Saccharomyces cerevisiae.. FEBS tetters 160:195–197
    [Google Scholar]
  24. Soderling T. R., Schworer C. M., Raafatel-Maghrabi M., Pilkis S. J. 1986; Phosphorylation of liver pyruvate kinase by calcium/calmodulin- dependent protein kinase.. Biochemical and Biophysical Research Communications 139:1017–1023
    [Google Scholar]
  25. Sora S., Bianchi M. 1982; Propranolol, atenolol and trifluoperazine reduce the spontaneous occurrence of meiotic diploid products in Saccharomyces cerevisiae.. Molecular and Cellular Biology 2:1299–1303
    [Google Scholar]
  26. Yamamoto H., Fukunaga K., Tanaka E., Miyamoto E. 1983; Calcium- and calmodulin- dependent phosphorylation of microtubule-associated protein 2 and τ factor, and inhibition of microtubule assembly.. Journal of Neurochemistry 41:1119–1125
    [Google Scholar]
  27. Warburg O., Christian W. 1941; Isolierung und Kristallisation des Gärungsferments Enolase.. Biochemische Zeitschrift 310:384–421
    [Google Scholar]
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