1887

Abstract

A small plasmid of 3·95 kb, encoding resistance to chloramphenicol (Cm) was detected in three of 33 strains. The plasmid in each of the three strains was indistinguishable by Southern-blot hybridization and restriction enzyme analysis. It was shown by curing and by transformation to specify resistance to Cm. A preliminary restriction map of the plasmid, designated pSC2, is presented. Chloramphenicol acetyltransferase was demonstrated by enzyme assay and by SDS-PAGE of cell-free lysates of pSC2 transformants.

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1989-12-01
2021-08-06
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