Cloning of a Thermostable α-Amylase Gene from Thermomonospora curvata and Its Expression in Streptomyces lividans

Summary: The gene from Thermomonospora curvata CCM 3312 coding for thermostable α-amylase (tarn) has been cloned in Streptomyces lividans TK 24 and localized to a 2·6 kb HindIII-BamHI fragment of DNA. The data presented here show that the tarn gene is expressed at a high level in S. lividans and that the protein is efficiently excreted.