1887

Abstract

Summary: The gamma radiation inactivation kinetics for at — 79°C were exponential. The radiation dose needed to reduce the number of infective by 90% varied from 0·64 to 1·2 kGy depending on the phase of the micro-organism, purity of the culture and composition of suspending menstruum. The viability of preparations containing 10C. mlwas completely abolished by 10 kGy without diminishing antigenicity or ability to elicit a protective immune response in vaccinated mice. Immunocytochemical examinations using monoclonal antibodies and electron microscopy demonstrated that radiation doses of 20 kGy did not alter cell-wall morphology or cell-surface antigenic epitopes.

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/content/journal/micro/10.1099/00221287-135-12-3263
1989-12-01
2019-10-21
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-12-3263
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