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Microbiology Society logo Microbiology

Volume 135, Issue 12

Novel Aerobactin Receptor in Free

Abstract

Several strains which produced enterochelin but not aerobactin were nevertheless sensitive to cloacin DF13. In contrast, a strain of serotype Kl:O1 which produced both siderophores was cloacin-resistant. Loss by mutation of the O1 but not Kl antigen rendered this strain cloacin-sensitive, indicating that the O1 antigen prevented access of cloacin to the cloacin/aerobactin receptor. Unlike the Kl:O1 strain, the aerobactin-negative strains failed to hybridize in a colony blot assay with an aerobactin receptor gene probe prepared from pColV-K30. However, antisera raised against the 74 kDa pColV-K30 aerobactin receptor cross-reacted with a 76 kDa outer-membrane protein in each strain. In addition to the 76 kDa protein, the K1:O1 strain also produced a strongly cross-reacting 74 kDa protein. To determine whether these aerobactin-negative strains could use aerobactin, mutants unable to synthesize siderophores were isolated. Aerobactin promoted the growth of these mutants in iron-deficient media. The evidence presented suggests that some strains produce an aerobactin iron-uptake system without apparent production of aerobactin and which is probably based on a 76 kDa receptor, the gene for which does not hybridize with aerobactin receptor gene encoded on pColV-K30.

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© Society for General Microbiology 1989
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1989-12-01
2025-05-15
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References

  1. Arnold L. D., Viswanatha T. 1983; The use of bis(mercaptoacetato-S,0)hydroxoiron(III) complex for the determination of hydroxamates.. Journal of Biochemical and Biophysical Methods 8:307–320
     [Google Scholar]
  2. Arnow L. E. 1937; Colorimetric determination of the components of 3,4-dihydroxyphenylalanine-tyro- sine mixtures.. Journal of Biological Chemistry 228:531–537
     [Google Scholar]
  3. Bagg A., Neilands J. B. 1987; Molecular mechanism of regulation of siderophore-mediated iron assimilation.. Microbiological Reviews 51:509–518
     [Google Scholar]
  4. Benedi V. J., Clurana B., Tomas J. M. 1989; Isolation and characterization of Klebsiella pneumoniae unencapsulated mutants.. Journal of Clinical Microbiology 27:82–87
     [Google Scholar]
  5. Bindereif A., Neilands J. B. 1983; Cloning of the aerobactin-mediated iron assimilation system of plasmid ColV.. Journal of Bacteriology 153:1111–1113
     [Google Scholar]
  6. Braun V., Brazel-Faisst C., Schneider R. 1984; Growth stimulation of Escherichia coli in serum by iron (III) aerobactin. Recycling of aerobac-tin.. FEMS Microbiology Letters 21:99–103
     [Google Scholar]
  7. Bullen J. J., Griffiths E. 1987 Iron and Infection; Molecular, Physiological and Clinical Aspects New York: John Wiley;
     [Google Scholar]
  8. Bywater M., Bywater R., Hellman L. 1983; A novel chromatographic procedure for purification of bacterial plasmids.. Analytical Biochemistry 132:219–224
     [Google Scholar]
  9. Carbonetti N. H., Williams P. H. 1984; A cluster of five genes specifying the aerobactin iron uptake system of plasmid ColV-K30.. Infection and Immunity 46:7–12
     [Google Scholar]
  10. Carbonetti N. H., Williams P. H. 1985; Detection of synthesis of the hydroxamate sidero- phore aerobactin by pathogenic isolates of Escherichia coli.. In The Virulence of Escherichia coli, Reviews and Methods pp. 419–424 Sussman M. Edited by New York: Academic Press;
     [Google Scholar]
  11. Carbonetti N. H., Boonchai S., Parry S. H., Vaisanen-Rhen V., Korhonen T., Williams P. H. 1986; Aerobactin-mediated iron-uptake by Escherichia coli isolates from human extra-intestinal infections.. Infection and Immunity 51:966–968
     [Google Scholar]
  12. Cercenado E., Baquero F., Delgado-Iribarren A., Martinez J. L. 1986; Epidemiology of aerobactin production in Enterobacteriaceae.. Annals de I’lnstitut Pasteur j Microbiologie 137B:297–303
     [Google Scholar]
  13. Chart H., Griffiths E. 1985; Antigenic and molecular homology of the ferric-enterobactin receptor protein of Escherichia coli.. Journal of General Microbiology 131:1503–1509
     [Google Scholar]
  14. Colonna B., Nicoletti M., Visca P., Casalino M., Valenti P., Maimone F. 1985; Composite IS7 elements encoding hydroxamate-mediated iron-uptake in FI me plasmids from epidemic Salmonella spp.. Journal of Bacteriology 162:307–316
     [Google Scholar]
  15. Crosa L. M., Wolf M. K., Actis L. A., Sanders-Loehr J., Crosa J. G. 1988; New aerobactin- mediated iron uptake system in a septicaemia- causing strain of Enterobacter cloacae.. Journal of Bacteriology 170:5539–5544
     [Google Scholar]
  16. Feinberg A. P., Vogelstein B. 1984; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity.. Analytical Biochemistry 137:266–267
     [Google Scholar]
  17. Fernandez-Beros M. E., Kissel V., Cabello F. C. 1988; Aerobactin-mediated iron-uptake system in intestinal Escherichia coli.. FEMS Microbiology Letters 49:397–401
     [Google Scholar]
  18. Gibson F., Magrath D. I. 1969; The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-1.. Biochimica et biophysica acta 192:175–184
     [Google Scholar]
  19. Griffiths E. 1987; The iron-uptake systems of pathogenic bacteria.. In Iron and Infection; Molecular, Physiological and Clinical Aspects pp. 69–137 Bullen J. J., Griffiths E. Edited by New York: John Wiley;
     [Google Scholar]
  20. Griffiths E., Stevenson P., Hale T. L., Formal S. B. 1985; Synthesis of aerobactin and a 76,000 dalton iron-regulated outer membrane protein by Escherichia coli-Kl2-Shigella hybrids and by enteroinvasive strains of Escherichia coli.. Infection and Immunity 49:67–71
     [Google Scholar]
  21. Gross R., Engelbrecht F., Braun V. 1985; Identification of the genes and their polypeptide products responsible for aerobactin synthesis by pColV plasmids.. Molecular and General Genetics 201:204–212
     [Google Scholar]
  22. Khimji P. L., Miles A. A. 1978; Microbial iron- chelators and their action on Klebsiella infections in the skin of guinea-pigs.. British Journal of Experi-mental Pathology 59:137–147
     [Google Scholar]
  23. Konopka K., Bindereif A., Neilands J. B. 1982; Aerobactin-mediated utilization of transferrin iron.. Biochemistry 21:6503–6508
     [Google Scholar]
  24. Krone W.J.A., Koningstein G., De Graaf F. K., Oudega B. 1985; Plasmid-determined cloacin DF 13-susceptibility in Enterobacter cloacae and Klebsiella edwardsii; identification of the cloacin DF13/aerobactin outer membrane receptor proteins.. Antonie van Leeuwenhoek 51:203–218
     [Google Scholar]
  25. Lodge J.M.T., Williams P., Brown M.R.W. 1986; Influence of growth rate and iron limitation on the expression of outer membrane proteins and enterobactin by Klebsiella pneumoniae grown in continuous culture.. Journal of Bacteriology 165:353–356
     [Google Scholar]
  26. Marolda C. L., Valvano M. A., Lawlor K. M., Payne S. M., Crosa J. H. 1987; Flanking and internal regions of chromosomal genes mediating aerobactin iron uptake systems in enteroinvasive Escherichia coli and Shigella flexneri.. Journal of General Microbiology 133:2269–2278
     [Google Scholar]
  27. Martinez J. L., Cercenado E., Baquero F., Perez-Diaz J. C., Delgado-Iribarren A. 1987; Incidence of aerobactin production in Gram-negative hospital isolates.. FEMS Microbiology Letters 43:351–353
     [Google Scholar]
  28. Nassif X., Sansonetti P. J. 1986; Correlation of the virulence of Klebsiella pneumoniae K1 and K2 with the presence of a plasmid encoding aerobactin.. Infection and Immunity 54:603–608
     [Google Scholar]
  29. Neilands J. B. 1981; Microbial iron compounds.. Annual Review of Biochemistry 50:715–731
     [Google Scholar]
  30. Neilands J. B. 1982; Microbial envelope proteins related to iron.. Annual Review of Microbiology 36:285–309
     [Google Scholar]
  31. Neilands J. B. 1984; Methodology of siderophores.. Structure and Bonding 58:1–24
     [Google Scholar]
  32. Payne S. M. 1980; Synthesis and utilization of siderophores by Shigella flexneri.. Journal of Bacteriology 143:1420–1424
     [Google Scholar]
  33. Roberts M., Parthasarathy S., Lam-Po-Tang M.K.L., Williams P. H. 1986; The aerobactin iron-uptake system in enteropathogenic Escherichia coli: evidence for an extinct transposon.. FEMS Microbiology Letters 37:215–219
     [Google Scholar]
  34. Schwyn B., Neilands J. B. 1987; Universal chemical assay for the detection and determination of siderophores.. Analytical Biochemistry 160:47–56
     [Google Scholar]
  35. Singh L., Jones K. W. 1984; The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures.. Nucleic Acids Research 12:5627–5638
     [Google Scholar]
  36. Stuart S. J., Prpic J. K., Robins-Browne R. M. 1986; Production of aerobactin by some species of the genus Yersinia.. Journal of Bacteriology 166:1131–1133
     [Google Scholar]
  37. Tomas J. M., Benedi V. J., Ciurana B., Jofre J. 1986; Role of capsule and O-antigen in resistance of K. pneumoniae to serum bactericidal activity.. Infection and Immunity 54:85–89
     [Google Scholar]
  38. Tomas J. M., Camprubi S., Williams P. 1988; Surface exposure of the O-antigen in Klebsiella pneumoniae K.1 :O1 serotype strains.. Microbial Pathogenesis 5:141–147
     [Google Scholar]
  39. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
     [Google Scholar]
  40. Valvano M. A., Silver R. P., Crosa J. H. 1986; Occurrence of chromosome- or plasmid-mediated aerobactin iron transport systems and hemolysin production amongst clonal groups of human invasive strains of Escherichia coli Kl.. Infection and Immunity 52:192–199
     [Google Scholar]
  41. Van Tiel-Menkveld C. J., Mentjox-Vervuust J. K., Oudega B., Degraaf F. K. 1982; Siderophore production by Enterobacter cloacae and a common receptor protein for the uptake of aerobactin and cloacin DF13.. Journal of Bacteriology 150:490–497
     [Google Scholar]
  42. Warner P. J., Williams P. H., Bindereif A., Neilands J. B. 1981; ColV plasmid-specified aerobactin synthesis by invasive strains of Escherichia coli.. Infection and Immunity 33:540–545
     [Google Scholar]
  43. Waters V. L., Crosa J. H. 1988; Divergence of the aerobactin iron-uptake systems encoded by plasmids pColV-K30 in Escherichia coli K12 and pSMNl in Aerobacter aerogenes 62-1.. Journal of Bacteriology 170:5153–5160
     [Google Scholar]
  44. Weislander L. 1979; A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.. Analytical Biochemistry 98:305–309
     [Google Scholar]
  45. Williams P. 1988; Role of the cell envelope in bacterial adaptation to growth in vivo in infections.. Biochimie 70:987–1011
     [Google Scholar]
  46. Williams P., Brown M.R.W., Lambert P. A. 1984; Effect of iron deprivation on the production of siderophores and outer membrane proteins in Klebsiella aerogenes.. Journal of General Microbiology 130:2357–2365
     [Google Scholar]
  47. Williams P., Chart H., Griffiths E., Stevenson P. 1987; Expression of high affinity iron uptake systems by clinical isolates of Klebsiella.. FEMS Microbiology Letters 44:407–412
     [Google Scholar]
  48. Williams P. H. 1979; Novel iron uptake system specified by ColV plasmids; an important component in the virulence of invasive strains of Escherichia coli.. Infection and Immunity 26:925–932
     [Google Scholar]
  49. Williams P. H., Carbonetti N. H. 1986; Iron, siderophores and the pursuit of virulence: independence of the aerobactin and enterochelin iron uptake systems in Escherichia coli.. Infection and Immunity 51:942–947
     [Google Scholar]
/content/journal/micro/10.1099/00221287-135-12-3173
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Novel Aerobactin Receptor in Klebsiella pneumoniae
Microbiology 135, 3173 (1989); https://doi.org/10.1099/00221287-135-12-3173
/content/journal/micro/10.1099/00221287-135-12-3173
/content/journal/micro/10.1099/00221287-135-12-3173
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