1887

Abstract

The ultrastructure of the infectious and reproductive forms of , a bacterium colonizing the macronucleus of , was analysed with the aid of ultrathin sections, freeze-fracture, freeze-etching and cytochemical electron microscopical techniques. The infectious form differed considerably from the reproductive form. The bacterial cytoplasm of the infectious form was confined to less than half of the cell, the rest being occupied by a voluminous periplasmic space. The periplasmic space contained a highly osmiophilic fine granular material. At the end of the cell distal from the cytoplasm, a tip containing less osmiophilic fine granular material was observed. Freeze-fracture and freeze-etching studies revealed differences in the patterns of intramembranous particles between the two forms. It is suggested that some of the structures characterizing the infectious form have a function in the infection process.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-11-3079
1989-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/11/mic-135-11-3079.html?itemId=/content/journal/micro/10.1099/00221287-135-11-3079&mimeType=html&fmt=ahah

References

  1. Bendayan M. 1984; Enzyme-gold electron microscopy cytochemistry: a new affinity approach for the ultrastructural localization of macromolecules. Journal of Electron Microscopic Technique 1:349–372
    [Google Scholar]
  2. Frens G. 1973; Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature; London: 24120–22
    [Google Scholar]
  3. Fujishima M., Fujita M. 1985; Infection and maintenance of Holospora obtusa, a macronucleus- specific bacterium of the ciliate Paramecium caudatum. Journal of Cell Science 76:179–187
    [Google Scholar]
  4. Görtz H. -D. 1983; Endonuclear symbionts in ciliates. In Intracellular Symbiosis pp. 145–176 Jeon K. W. Edited by New York: Academic Press;
    [Google Scholar]
  5. Görtz H. -D. 1986; Endonucleobiosis in ciliates. International Review of Cytology 102:169–213
    [Google Scholar]
  6. Görtz H. -D., Dieckmann J. 1980; Life cycle and infectivity of Holospora elegans (Hafkine), a micronucleus-specific symbiont of Paramecium caudatum (Ehrenberg). Protistologica 16:591–603
    [Google Scholar]
  7. Görtz H. -D., Wiemann M. 1987; Colonization of the ciliate Stentor multiformis by three different endocytobionts. Endocytobiology and Cell Research 4:177–184
    [Google Scholar]
  8. Görtz H. -D., Wiemann M. 1989; Route of infection of the bacteria Holospora elegans and Holospora obtusa into the nuclei of Paramecium caudatum. European Journal of Protistology 24:101–109
    [Google Scholar]
  9. Görtz H. -D., Freiburg M., Wiemann M. 1988; Polypeptide differences between infectious and reproductive forms of Holospora obtusa, an endo-nucleobiotic bacterium from the macronucleus of Paramecium caudatum. Endocytobiology and Cell Research 5:233–244
    [Google Scholar]
  10. Gromov B. V., Ossipov D. V. 1981; Holospora (ex Hafkine 1890) nom. rev., a genus of bacteria inhabiting the nuclei of paramecia. International Journal of Systematic Bacteriology 31:348–352
    [Google Scholar]
  11. Gromov B. V., Mamkaeva K. A., Ossipov D. V. 1976; Ultrastructure of the I-particles - symbiotic bacteria of the macronucleus of Paramecium caudatum (Protozoa, Ciliata). Izvestija Akademia Nauk SSSR Series Biologiya 3:399–409
    [Google Scholar]
  12. Moulder J. W. 1984; Chlamydiales. In Bergey’s Manual of Systematic Bacteriology 1 pp. 729–739 Krieg N. R. Edited by Baltimore & London: Williams & Wilkins;
    [Google Scholar]
  13. Ossipov D. V., Skoblo I. I., Rautian M. S. 1975; Iota-particles, macronuclear symbiotic bacteria of ciliate Paramecium caudatum clone M-115. Acta protozoologica 14:263–280
    [Google Scholar]
  14. Preer J. R. Jr 1984; Endosymbionts of Protozoa. In Bergey’s Manual of Systematic Bacteriology 1 pp. 795–813 Krieg N. R. Edited by Baltimore & London: Williams & Wilkins;
    [Google Scholar]
  15. Schmidt H. J., Freiburg M., Görtz H. -D. 1987; Comparison of the infectious forms of two bacterial endonucleobionts, Holospora elegans and H. obtusa, from the ciliate Paramecium caudatum. Microbios 49:189–197
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-11-3079
Loading
/content/journal/micro/10.1099/00221287-135-11-3079
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error