RT Journal Article SR Electronic(1) A1 Kanda, Kohzo A1 Tan, Yepin A1 Aizawa, KeioYR 1989 T1 A Novel Phage Genome Integrated into a Plasmid in Bacillus thuringiensis Strain AF101 JF Microbiology, VO 135 IS 11 SP 3035 OP 3041 DO https://doi.org/10.1099/00221287-135-11-3035 PB Microbiology Society, SN 1465-2080, AB Bacillus thuringiensis strain AF101 possesses a single plasmid (pAF101) with a molecular size of 42 MDa (69 kb). During plasmid curing experiments in strain AF101, we found that a phage (J7W-1) was induced by ethidium bromide treatment. Moreover, the phage genome (48 kb) hybridized only with pAF101 on a Southern blot of the DNA of a cleared lysate prepared from strain AF101. Comparison of the restriction patterns of pAF101 and J7W-1 phage DNA revealed that pAF101 contains not only the entire phage DNA but also a plasmid-specific DNA region. These results indicate that the J7W-1 genome has been stably integrated into pAF101 in strain AF101. Integration of the J7W-1 genome into a plasmid was also observed after phage infection of the type strain of B. thuringiensis subsp. israelensis. , UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-11-3035