@article{mbs:/content/journal/micro/10.1099/00221287-135-11-3035, author = "Kanda, Kohzo and Tan, Yepin and Aizawa, Keio", title = "A Novel Phage Genome Integrated into a Plasmid in Bacillus thuringiensis Strain AF101", journal= "Microbiology", year = "1989", volume = "135", number = "11", pages = "3035-3041", doi = "https://doi.org/10.1099/00221287-135-11-3035", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-11-3035", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = " Bacillus thuringiensis strain AF101 possesses a single plasmid (pAF101) with a molecular size of 42 MDa (69 kb). During plasmid curing experiments in strain AF101, we found that a phage (J7W-1) was induced by ethidium bromide treatment. Moreover, the phage genome (48 kb) hybridized only with pAF101 on a Southern blot of the DNA of a cleared lysate prepared from strain AF101. Comparison of the restriction patterns of pAF101 and J7W-1 phage DNA revealed that pAF101 contains not only the entire phage DNA but also a plasmid-specific DNA region. These results indicate that the J7W-1 genome has been stably integrated into pAF101 in strain AF101. Integration of the J7W-1 genome into a plasmid was also observed after phage infection of the type strain of B. thuringiensis subsp. israelensis. ", }