1887

Abstract

Polyacrylamide gel electrophoresis of extracellular pectinase and amylase isozymes of 170 mainly terverticillate strains was undertaken. The data were coded and subjected to numerical analysis. Variation in intensity of isozymes was observed in repeat analyses of some strains, although most were consistent. Variation was also observed between some representative strains of species. was more variable than and for intensity of pectinase activity. There was a correlation between the grouping of the strains on the basis of the isozymes and the species concepts only in some cases. The method proved useful for the identification of strains producing intense activity which provided clear patterns, for example, and and to a lesser extent var. and . The method was also exclusionary in that some species were restricted to a particular cluster or subcluster. Amylase patterns confirmed that strains referred to as single species are not all homogeneous genetically, and that some strains are not simply haploid homokaryons. The genetic heterogeneity of the strains explains some of the problems in the systematics of the terverticillate penicillia.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-11-2979
1989-11-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/11/mic-135-11-2979.html?itemId=/content/journal/micro/10.1099/00221287-135-11-2979&mimeType=html&fmt=ahah

References

  1. Anné J., Peberdy J. F. 1981; Characterization of inter-specific hybrids between Penicillium chrysogenum and P. roqueforti by isoenzyme analysis. Transactions of the British Mycological Society 77:401–408
    [Google Scholar]
  2. Ball C. 1971; Haploidization analysis in Penicillium chrysogenum . Journal of General Microbiology 66:63–69
    [Google Scholar]
  3. Bridge P. D., Hawksworth D. L., Kozakiewicz Z., Onions A.H.S., Paterson R.R.M. 1986; Morphological and biochemical variation in single isolates of Penicillium . Transactions of the British Mycological Society 87:389–396
    [Google Scholar]
  4. Bridge P. D., Hudson L., Kozakiewicz Z., Onions A.H.S., Paterson R.R.M. 1987; Investigations of variation in phenotype and DNA content between single-conidium isolates of single Penicillium strains. Journal of General Microbiology 133:996–1004
    [Google Scholar]
  5. Bridge P. D., Hawksworth D. L., Kozakiewicz Z., Onions A.H.S., Paterson R.R.M., Sackin M. J., Sneath P.H.A. 1989; A reappraisal of terverticillate penicillia using biochemical, physiological and morphological features. I. Numerical taxonomy. Journal of General Microbiology 135:2941–2966
    [Google Scholar]
  6. Crackower S., Bauer H. 1971; Mitosis in Penicillium chrysogenum and Penicillium notatum . Canadian Journal of Microbiology 17:605–608
    [Google Scholar]
  7. Cruickshank R. H., Wade G. C. 1980; Detection of pectin enzymes in pectin-acrylamide gels. Analytical Biochemistry 107:177–181
    [Google Scholar]
  8. Cruickshank R. H., Pitt J. I. 1987; Identification of species in Penicillium subgenus Penicillium by enzyme electrophoresis. Mycologia 79:614–620
    [Google Scholar]
  9. Garber E. D., Beraha L. 1966; Genetics of pathogenic fungi. Pectolytic enzymes of virulent and avirulent strains of 3 phytopathogenic penicillia. Canadian Journal of Botany 44:1645–1650
    [Google Scholar]
  10. Hamamoto M., Sugiyama J., Goto S., Komagata K. 1986; Numerical taxonomy based on the electrophoretic mobility of enzymes in the genera Rhodosporidium, Cystofilobasidium and Rhodotorula . Journal of General and Applied Microbiology 32:89–99
    [Google Scholar]
  11. Hepper C. M., Sen R., Azcon-Aguilar C., Grace C. 1988; Variation in certain isozymes amongst different geographical isolates of the vescicular-arbuscular mycorrhizal fungi Glomus clarum, Glomus monosporum, and Glomus mosseae . Soil Biology and Biochemistry 20:51–59
    [Google Scholar]
  12. Mays B., Roberts D. W., Soper R. S. 1979; Intraspecific genetic variability in laboratory strains of Entomophthora as determined by enzyme electrophoresis. Experimental Mycology 3289–297
    [Google Scholar]
  13. Micales J. A., Bonde M. R., Peterson G. L. 1986; The use of isozyme analysis in fungal taxonomy and genetics. Mycotaxon 27405–449
    [Google Scholar]
  14. Paterson R. R. M., King G., Bridge P. D. 1989; High resolution melting curves of DNA from 14 Penicillium strains . Mycological Research (in the Press)
    [Google Scholar]
  15. Smith D., Onions A.H.S. 1983 The Preservation and Maintenance of Living Fungi. Kew:: Commonwealth Mycological Institute.;
    [Google Scholar]
  16. Sneath P.H.A., Sokal R. R. 1973 Numerical Taxonomy. San Francisco:: W. H.Freeman.;
    [Google Scholar]
  17. Tolmsoff W. J. 1983; Heteroploidy as a mechanism of variability among fungi. Annual Review of Phytopathology 21:317–340
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-11-2979
Loading
/content/journal/micro/10.1099/00221287-135-11-2979
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error