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Volume 135, Issue 11

1,3--Glucanase, 1,6--GIucanase and -Glucosidase Activities of : Synthesis and Properties Free

Abstract

Summary: The filamentous fungus excreted significant amounts of 1,3--glucanase, 1,6--glucanase and --glucosidase activities when the culture medium was depleted of carbon sources. During starvation small amounts of intracellular 1,3-- and 1,6--glucanase and -glucosidase activities were also detected. Very low levels of -glucanase activity remained bound to mycelium and some activity was found loosely attached to the cells and/or to water-soluble 1,3--1,6--glucan adhering to the cell walls. During active growth intracellular 1,3--glucanase and mycelium-bound 1,3-- and 1,6--glucanase activities were detected in small or trace amounts. During hyphal growth very low levels of 1,3-- and 1,6--glucanase activities were also found to be weakly associated with the cells and/or with water-soluble -glucan covering the hyphae. Cycloheximide inhibited the increase in intra- and extracellular 1,3-- and 1,6--glucanase and -glucosidase activities. This indicated that protein synthesis was involved in the intra- and extracellular appearance of these three enzyme activities in derepressed cells. The formation of the extracellular 1,3--glucanase, 1,6--glucanase and -glucosidase activities was regulated by catabolite repression. 1,3-- and 1,6--glucanase activities were uncompetitively inhibited and -glucosidase activity noncompetitively inhibited by glucose and glucono-δ-lactone. Optimum pH and temperature values as well as thermal stabilities of the three extracellular enzyme activities were determined. Almost all of the -glucosidase activity but only one-third of the extracellular 1,3-- and 1,6--glucanase activities were found to bind to Con A-Sepharose. Under conditions of carbon limitation almost 90% of the extracellular 1,3--/1,6--glucan excreted during fungal growth was degraded by the extracellular -glucanases.

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© Society for General Microbiology 1989
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1989-11-01
2024-12-06
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1,3-β-Glucanase, 1,6-β-GIucanase and β-Glucosidase Activities of Sclerotium glucanicum: Synthesis and Properties
Microbiology 135, 2847 (1989); https://doi.org/10.1099/00221287-135-11-2847
/content/journal/micro/10.1099/00221287-135-11-2847
/content/journal/micro/10.1099/00221287-135-11-2847
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