Halocin H6, a Bacteriocin from Free

Abstract

strain Ma2.39 produces an inhibitor substance designated halocin H6. This halocin was isolated from culture supernatants of the producer strain. It appeared to be a non-inducible bacteriocin with a bactericidal mode of cation and typical ‘single-hit’ kinetics. The protein was purified by a combination of hydroxylapatite affinity chromatography, gel filtration and HPLC. Halocin H6 is a protein of 32000; it is heat resistant, non-salt-dependent (it retains its activity after exposure to distilled water) and sensitive to pronase but not to trypsin.

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1989-10-01
2024-03-29
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