1887

Abstract

HB and four adhesion deficient mutants, HB-7, HB-V5, HB-V51 and HB-B, were grown in continuous culture in a defined medium under glucose limitation over a range of growth rates from 0·1 to 1·1 h. The ability to coaggregate with V1 cells and the ability to adhere to buccal epithelial cells did not alter with increasing growth rate. Cell surface hydrophobicity decreased markedly with increasing growth rate for the non-fibrillar non-adhesive mutant HB-B but not for the other four strains which all carry different combinations of fibril classes. The thickness of the ruthenium red staining layer (RRL) also varied with growth rate for strain HB-B, ranging from 19·5 ± 3·8 nm at high growth rate to a minimum of 12·3 ± 4·8 nm at low growth rate. Low cell surface hydrophobicity correlated with a thicker RRL for strain HB-B. Strains HB-V5 and HB-7 also showed a significant increase in RRL thickness at high growth rates although to a lesser degree than HB-B. SDS-PAGE revealed a large number of protein bands common to all strains at all growth rates, with the major common protein occurring at 15·6 kDa. Protein bands at 70, 56, 40·5 and 39 kDa appeared stronger at high growth rates than at low. A protein band at 82 kDa showed strongly only at low growth rates. Therefore, adhesion and coaggregation are not phenotypically variable with increasing growth rate but RRL thickness, hydrophobicity and cell surface proteins may be phenotypically variable depending on the strain.

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1989-10-01
2022-01-17
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References

  1. Campbell L.K., Knox K.W., Wicken A.J. 1983; Influence of growth conditions on adherence of Streptococcus mutans Ingbritt to saliva-coated hydroxyapatite. Infection and Immunity 39:445–448
    [Google Scholar]
  2. Carlsson J., Elander B. 1973; Regulation of dextransucrase formation by Streptococcus sanguis. Caries Research89–101
    [Google Scholar]
  3. Christie A.O., Porteous J.W. 1962; The cultivation of a single strain of Actinomyces israelii in simplified chemically defined medium. Journal of General Microbiology 28:443–454
    [Google Scholar]
  4. Ellwood D.C., Hunter J.R. 1976; The mouth as a chemostat. In Continuous Culture 6: Applications and New Fields pp. 270–281 Dean A.C.R., Ellwood D.C., Evans C.G.T., Melling J. Edited by Chichester: Ellis Horwood;
    [Google Scholar]
  5. Handley P.S., Carter P.L., Fielding J. 1984; Streptococcus salivarius strains carry either fibrils or fimbriae on the cell surface. Journal of Bacteriology 157:64–72
    [Google Scholar]
  6. Handley P.S., Harty D.W.S., Wyatt J.E., Brown C.R., Doran J.P., Gibbs A.C.C. 1987; . A comparison of the adhesion, coaggregation and cell-surface hydrophobicity properties of fibrillar and fimbriate strains of Streptococcus salivarius . Journal of General Microbiology 133:3207–3217
    [Google Scholar]
  7. Handley P.S., Hargreaves J., Harty D.W.S. 1988; . Ruthenium red staining reveals surface fibrils and a layer external to the cell wall in Streptococcus salivarius HB and adhesion deficient mutants. Journal of General Microbiology 134:3165–3172
    [Google Scholar]
  8. Harty D.W.S., Handley P.S. 1988; Fermentation products, amino acid utilization, maintenance energies and growth yields for the fibrillar Streptococcus salivarius HB and a non-fibrillar mutant HB-B grown in continuous culture under glucose limitation. Journal of Applied Bacteriology 65:143–152
    [Google Scholar]
  9. Harty D.W.S., Handley P.S. 1989; The effect of changing growth rate on the expression of surface properties of fibrillar Streptococcus salivarius HB. Xth Lancefield International Symposium on Streptococci and Streptococcal Diseases, Koln, FRG. Lutticken R. Edited by Stuttgart & New York: Gustav Fischer; (in the Press)
    [Google Scholar]
  10. Hesketh L.M., Wyatt J.E., Handley P.S. 1987; Effect of protease on the cell surface structure, hydrophobicity and adhesion of tufted strains of Streptococcus sanguis biotypes I and II. Microbios 50:131–145
    [Google Scholar]
  11. Jacques N.A., Hardy L., Campbell L.K., Knox K.W., Evans D., Wicken A.J. 1979; Effect of carbohydrate source and growth conditions on the production of lipoteichoic acid by Streptococcus mutans Ingbritt. Infection and Immunity 26:1079–1087
    [Google Scholar]
  12. Jenkinson H.F. 1986; Cell-surface proteins of Streptococcus sanguis associated with cell hydrophobicity and coaggregation properties. Journal of General Microbiology 132:1575–1589
    [Google Scholar]
  13. Jenkinson H.F., Carter D.A. 1988; Cell surface mutants of Streptococcus sanguis with altered adherence properties. Oral Microbiology and Immunology 3:53–57
    [Google Scholar]
  14. Knox K.W., Jacques N.A., Campbell L.K., Wicken A.J., Hurst S.F., Bleiweis A.S. 1979; Phenotypic stability of the cell wall of Streptococcus mutans Ingbritt grown under various conditions. Infection and Immunity 26:1071–1078
    [Google Scholar]
  15. Knox K.W., Hardy L.N., Wicken A.J. 1986; Comparative studies on the protein profiles and hydrophobicity of strains of Streptococcus mutans serotype c . Journal of General Microbiology 132:2541–2548
    [Google Scholar]
  16. Laemmli U.K., Favre M. 1973; Maturation of the head of bacteriophage T4. 1. DNA packaging events. Journal of Molecular Biology 80:579–599
    [Google Scholar]
  17. Luft J.H. 1971; Ruthenium red and violet. 1. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anatomical Record 171:347–368
    [Google Scholar]
  18. Rogers A.H., Pilowsky K., Zilm P.S. 1984; The effect of growth rate on the adhesion of the oral bacteria Streptococcus mutans and Streptococcus milleri . Archives of Oral Biology 29:147–150
    [Google Scholar]
  19. Rosan B., Applebaum B., Campbell L.K., Knox K.W., Wicken A.J. 1982; Chemostat studies of the effect of environmental control on Streptococcus sanguis adherence to hydroxyapatite. Infection and Immunity 35:64–70
    [Google Scholar]
  20. Rosenberg M., Gutnick D., Rosenberg E. 1980; Adherence of bacteria to hydrocarbons: a simple method for measuring cell surface hydro-phobicity. FEMS Microbiology Letters 9:29–33
    [Google Scholar]
  21. Terleckyj B., Willett N.P., Shockman G.D. 1975; Growth of several cariogenic strains of oral streptotocci in a chemically defined medium. Infection and Immunity 11:649–655
    [Google Scholar]
  22. Weerkamp A.H., Handley P.S. 1986; The growth rate regulates the composition and density of the fibrillar coat on the surface of Streptococcus salivarius K+ cells. FEMS Microbiology Letters 33:179–183
    [Google Scholar]
  23. Weerkamp A.H., Jacobs T. 1982; Cell wall associated protein antigens of Streptococcus salivarius-. purification, properties and function in adherence. Infection and Immunity 38:233–242
    [Google Scholar]
  24. Weerkamp A.H., Mcbride B.C. 1981; Identification of a Streptotoccus salivarius cell wall component mediating coaggregation with Veillonella alcalescens VI. Infection and Immunity 32:723–730
    [Google Scholar]
  25. Weerkamp A.H., Van der mei H.C., Engelen D.P.E., Dewindt C.E.A. 1984; Adhesion receptors (adhesins) of oral streptococci. In Bacterial Adhesion and Preventative Dentistry pp. 85–97 Ten Cate J.M., Leach F.A., Arends J. Edited by Oxford: IRL Press;
    [Google Scholar]
  26. Weerkamp A.H., Handley P.S., Baars A., Slot J.W. 1986; Negative staining and immunoelectron microscopy of adhesion-deficient mutants of Streptococcus salivarius reveal that the adhesive protein antigens are separate classes of cell surface fibril. Journal of Bacteriology 165:746–755
    [Google Scholar]
  27. Weerkamp A.H., Van der mei H.C., Slot J.W. 1987; Relationship of cell surface morphology and composition of Streptococcus salivarius K+ to adherence and hydrophobicity. Infection and Immunity 55:438–445
    [Google Scholar]
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