Summary: PS5, a double-stranded DNA bacteriophage of JM604 that adsorbs specifically to the outer-membrane protein NosA, was isolated from stagnant irrigation ditch water. Mutant strains that do not produce NosA are resistant to PS5 and cannot grow anaerobically with NO as the sole electron acceptor. PS5 did not adsorb to mutants and adsorption to the wild-type strain was reduced when cells were grown with a high concentration of copper, a condition that represses the synthesis of NosA. The isolation of spontaneous PS5-resistant mutants yielded strains that were clearly defective in growth on NO at about a 10% incidence. About half of these strains could respire NO when supplied with a high concentration of exogenous copper.


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