1887

Abstract

The electrokaryotype of the pathogenic yeast is described for the first time. Three different patterns were seen: () serotypes B and C (variety ) are similar and consist of nine chromosome mobility groups of >580 kb; () serotype A (variety ) revealed eight chromosome-like groups > 700 kb; () serotype D (the second serotype of variety ) not only differs from those described above, but each D isolate tested showed a different distribution of bands. The discrepancy, and the importance of electrokaryotyping as a taxonomic tool, are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-1-65
1989-01-01
2024-11-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/1/mic-135-1-65.html?itemId=/content/journal/micro/10.1099/00221287-135-1-65&mimeType=html&fmt=ahah

References

  1. Bhattacharjee A.K., Bennett J.E., Glaude-Mans C.P.J. 1984; Capsular polysaccharides ofCryptococcus neoformans. Reviews of Infectious Diseases 6:619–642
    [Google Scholar]
  2. Butler W.T., Alling D.W., Anderson J., Utz J.P. 1964; Diagnostic and prognostic value of clinical and laboratory finding in cryptococcal meningitis. New England Journal of Medicine 270:59–67
    [Google Scholar]
  3. Carle G.F., Olson M.V. 1984; Separation of chromosomal DNA molecules from yeast by orthogonal field gel electrophoresis. Nucleic Acids Research 12:5647–5664
    [Google Scholar]
  4. Carle G.F., Olson M.V. 1985; An electrophoretic karyotype for yeast. Proceedings of the National Academy of Sciences of the United States of America 82:3756–3780
    [Google Scholar]
  5. Chu G., Vollrath D., Davis R.W. 1986; Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234:1582–1585
    [Google Scholar]
  6. Kwon-Chung K. J. 1976a; Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia 688:821–833
    [Google Scholar]
  7. Kwon-Chung K.J. 1976; A new species of Filobasidiella, the sexual stage of Cryptococcus neoformans B and C serotypes. Mycologia 689:942–946
    [Google Scholar]
  8. Kwon-Chung K.J., Bennett J.E. 1984; Epidemiologic differences between the two varieties of Cryptococcus neoformans. Journal of Epidemiology 120:123–130
    [Google Scholar]
  9. Kwon-Chung K. J., Fell J. W. 1984; Filobasidiella Kwon-Chung. In The Yeasts: a TaxonomicStudy, 3rd. pp. 472–482 Kreger-van Rij N. J. W. Edited by Amsterdam:: Elsevier Science Publishers.;
    [Google Scholar]
  10. Kwon-Chung K.J., Rhodes J.C. 1986; Encapsulation and melanin formation as indicators of virulence in Cryptococcus neoformans. Infection and Immunity 51:218–223
    [Google Scholar]
  11. Kwon-Chung K.J., Bennett J. E., Rhodes J. C. 1982a; Taxonomic studies on Filobasidiella species and their anamorphs. Antonie Van Leeuwenhoek 48:2538
    [Google Scholar]
  12. Kwon-Chung K.J., Polacheck I., Popkin T.J. 1982; Melanin-lacking mutants of Cryptococcus neoformans and their virulence for mice. Journal of Bacteriology 150:1414–1421
    [Google Scholar]
  13. Lott T.J., Boiron P., Reiss E. 1987; An electrophoretic karyotype for Candida albicans reveals large chromosomes in multiples. Molecular and General Genetics 209:170–174
    [Google Scholar]
  14. Magee B.B., Magee P.T. 1987; Electrophoretic karyotypes and chromosome numbers in Candidaspecies. Journal of General Microbiology 133:425–430
    [Google Scholar]
  15. Mortimer R.K., Schild D. 1985; Genetic map of Saccharomyces cerevisiae, edition 9. Microbiological Reviews 49:181–212
    [Google Scholar]
  16. Polacheck I., Kwon-Chung K.J. 1988; Melanogenesis in Cryptococcus neoformans. Journal of General Microbiology 134:1037–1041
    [Google Scholar]
  17. Polacheck I., Hearing V.J., Kwon-Chung K.J. 1982; Biochemical studies of phenoloxidase and utilization of catecholamines in Cryptococcus neoformans. Journal of Bacteriology 150:1212–1220
    [Google Scholar]
  18. Quinn T. C., Mann J. W., Curran J. W., Piot P. 1986; AIDS in Africa : an epidemiologic paradigm. Science 234:955–963
    [Google Scholar]
  19. Rhodes J.C., Polacheck I., Kwon-Chung K.J. 1982; Phenoloxidase activity and virulence in isogenic strains of Cryptococcus neoformans. Infection and Immunity 36:1175–1184
    [Google Scholar]
  20. Smith C.L., Matsumoto T., Niwa O., Klco S., Fan J.B., Yanagida M., Cantor C.R. 1987; An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis. Nucleic Acids Research 15:4481–4489
    [Google Scholar]
  21. Snell R.G., Wilkins R.J. 1986; Separation of chromosomal DNA molecules from Candida albicansby pulsed field gel electrophoresis. Nucleic Acids Research 14:4401–4406
    [Google Scholar]
  22. Vollrath D., Davis R.W. 1987; Resolution of DNA molecules greater than 5 megabases by contour-clamped homogenous electric fields. Nucleic Acids Research 15:7865–7876
    [Google Scholar]
  23. Whelan W.L., Kwon-Chung K.J. 1986; Genetic complementation in Cryptococcus neoformans. Journal of Bacteriology 166:924–929
    [Google Scholar]
  24. White C.W., Jacobson E.S. 1985; Occurrence of diploid strains of Cryptococcus neoformans. Journal of Bacteriology 161:1231–1232
    [Google Scholar]
  25. Zuger A., Louie E., Holzman R.S., Simberkoff M.S., Rahal J.J. 1986; Cryptococcal disease in patients with the Acquired Immunodeficiency Syndrome. Annals of Internal Medicine 104:234–240
    [Google Scholar]
/content/journal/micro/10.1099/00221287-135-1-65
Loading
/content/journal/micro/10.1099/00221287-135-1-65
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error