1887

Abstract

The transport systems for glucose present in cells, growing in batch and continuous cultures on several carbon sources, have been studied. Two different systems were found: a proton symport and a facilitated diffusion system. The high-affinity symport ( for glucose about 15 ) transported one proton per mole of glucose and was partially constitutive, appearing in cells grown on gluconeogenic substrates such as lactate, ethanol and glycerol. It was also induced by glucose concentrations up to 07 m and repressed by higher ones. The level of repression depended on the external glucose concentration at which cells had grown in a way similar to that shown by the maltose-uptake system, so both systems seem to be under a common glucose control. Initial uptake by facilitated diffusion, the only transport system present in cells growing at glucose concentrations higher than 10 m, showed a complex kinetic dependence on the extracellular glucose concentration. This could be explained either by the presence of at least two different systems simultaneously active, one with a around 2 m and the other with a of about 1 , or by the allosteric or hysteretic behaviour of a single carrier whose apparent would oscillate between 2 and 70 m.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-1-195
1989-01-01
2021-05-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/1/mic-135-1-195.html?itemId=/content/journal/micro/10.1099/00221287-135-1-195&mimeType=html&fmt=ahah

References

  1. Barnett J.R., Sims A.P. 1976; A note on the kinetics of uptake of d-glucose by the food yeast, Candida utilis. Archives of Microbiology 111:193–194
    [Google Scholar]
  2. Bisson L.F., Fraenkel D.G. 1984; Expression of kinase-dependent glucose uptake in Saccharomyces cerevisiae. Journal of Bacteriology 159:1013–1017
    [Google Scholar]
  3. Busturia A., Lagunas R. 1986; Catabolite inactivation of the glucose transport system in Saccharomyces cerevisiae. Journal of General Microbiology 132:379–385
    [Google Scholar]
  4. Eddy A.A. 1982; Mechanisms of solute transport in selected eukaryotic microorganisms. Advances in Microbial Physiology 24:1–79
    [Google Scholar]
  5. Eddy A.A., Hopkins P.G. 1985; The putative electrogenic nitrate-proton symport of the yeast Candida utilis. Biochemical Journal 231:291–297
    [Google Scholar]
  6. Gasnier B. 1987; Characterization of low- and high-affinity glucose transports in the yeast Kluyveromyces marxianus. Biochimica et biophysica acta 903:425–433
    [Google Scholar]
  7. Herbert D., Phipps P.J., Strange R.E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:210–344
    [Google Scholar]
  8. Hofer M., Misra P.C. 1978; Evidence for a proton-sugar symport in the yeast Rhodotorula gracilis (glutinis). Biochemical Journal 172:15–22
    [Google Scholar]
  9. Loureiro-Días M.C. 1987; Glucose and polyol transport systems in Candida intermedia and their regulation. Journal of General Microbiology 133:2737–2742
    [Google Scholar]
  10. Loureiro-Días M.C., Peinado J.M. 1984; Transport of maltose in Saccharomyces cerevisiae.Effect of pH and potassium ions. Biochemical Journal 222:293–298
    [Google Scholar]
  11. Lucas C., Van Uden N. 1986; Transport of hemicellulose monomers in the xylose fermenting yeast Candida sheatae 23. Applied Microbiology and Biotechnology 23:491–495
    [Google Scholar]
  12. Peinado J.M., Barbero A., Van Uden N. 1987; Repression and inactivation by glucose of the maltose transport system of Candida utilis. Applied Microbiology and Biotechnology 26:154–157
    [Google Scholar]
  13. Postma E., Van Dijken J.P., Scheffers W.A. 1987; Kinetics of glucose uptake in carbon limited chemostat cultures of Candida utilis. In Proceedings of the 4th European Congress on Biotechnology 3 p. 368 Neijssel O. M., Van der Meer R. R., Luyben K. Ch. A. M. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  14. Spencer-Martins I., Van Uden N. 1985; Catabolite interconversion of glucose transport systems in the yeast Candida wickerhamii. Biochimicaetbiophysicaacta 812:168–172
    [Google Scholar]
  15. Van Uden N. 1967; Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition. Archives of Microbiology 58:155–168
    [Google Scholar]
  16. Verma R.S., Spencer-Martins I., Van Uden N. 1987; Role of de novo protein synthesis in the interconversion of glucose transport systems in the yeast Pichia ohmeri. Biochimica et biophysica acta 900:139–144
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-1-195
Loading
/content/journal/micro/10.1099/00221287-135-1-195
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error