1887

Abstract

strain CB 1809 assimilated and denitrified NO simultaneously to NH and N, respectively, when incubated under anaerobiosis. Washed cells from cultures grown in air in the presence of 5 mM-nitrate, assimilated 70% of the NO into cell-nitrogen but only after a lag of 10 h during which time NO accumulated. In washed cells grown at 2% (v/v) O with 5 m-nitrate, NO was denitrified to N (95%) in 1–2 h. The low O tension in the culture medium resulted in increased activity of nitrate and nitrite reductases in the cells. When 10 m-glutamate was included with 5 m-nitrate at 2% (v/v) O, washed cells from these cultures had less nitrate and nitrite reductase activities and when NO was supplied to these cells there was a transient accumulation of NO (1 h) prior to maximum denitrification. Washed cells prepared from cultures grown at 2% (v/v) O with glutamate alone utilized NO , only after a lag period of 20-40 h, assimilation by (60%) and denitrification (40%).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-1-189
1989-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/1/mic-135-1-189.html?itemId=/content/journal/micro/10.1099/00221287-135-1-189&mimeType=html&fmt=ahah

References

  1. Bergersen F.J. 1977; Physiological chemistry of dinitrogen fixation by legumes. In A Treatise on Dinitrogen Fixation 3 pp 519–555 Hardy R. W. F., Silver W. S. Edited by New York: John Wiley and Sons;
    [Google Scholar]
  2. Brownell P.F., Nicholas D.J.D. 1967; Some effects of sodium on nitrate assimilation and N2 fixation in Anabaena cylindrica. Plant Physiology 42:915–921
    [Google Scholar]
  3. Byrne M.D., Nicholas D.J.D. 1986; Multiple-phase equilibration head space analysis for the determination of N2O and N2 during bacterial denitrification. Analytical Biochemistry 154:470–475
    [Google Scholar]
  4. Cataldo D.A., Harron M., Schrader L.E., Youngs V.L. 1975; Rapid colorimetric determination of nitrate in plant tissues by nitration of salicylic acid. Communication in Soil Science and Plant Analysis 6:71–80
    [Google Scholar]
  5. Conway E.J. 1962 Microdiffusion Analysis and Volumetric Error. London: Crosby, Lockwood and Son;
    [Google Scholar]
  6. Dalton H. 1980; The cultivation of diazotrophic microorganisms. In Methods for Evaluating Biological Nitrogen Fixation, pp 13–64 Chichester: John Wiley and Sons;
    [Google Scholar]
  7. Daniel R.M., Limmer A.W., Steele K.W., Smith J.M. 1982; Anaerobic growth, nitrate reduction and denitrification in 46 Rhizobium strains. Journal of General Microbiology 128:1811–1815
    [Google Scholar]
  8. Itzhaki R.F., Gill D.M. 1964; A microbiuret method for estimating proteins. Analytical Biochemistry 9:401–410
    [Google Scholar]
  9. Kapralék F., Jechova E., Otavova M. 1982; Two sites of oxygen control in induced synthesis of respiratory nitrate reductase in Escherichia coli. Journal of Bacteriology 149:1142–1145
    [Google Scholar]
  10. Knowles R. 1982; Denitrification. Microbiological Reviews 46:43–70
    [Google Scholar]
  11. Krul J.M., Veeningen R. 1977; The synthesis of the dissimilatory nitrate reductase under aerobic conditions in a number of denitrifying bacteria, isolated from activated sludge and drinking water. Water Research 11:39–43
    [Google Scholar]
  12. Lam Y., Nicholas D.J.D. 1969; A nitrate reductase from Micrococcus denitrificans. Biochimica et biophysica acta 178:225–234
    [Google Scholar]
  13. Markham R. 1942; A steam distillation apparatus suitable for micro-kjeldahl analysis. Journal of Biochemistry 36:790–791
    [Google Scholar]
  14. Meiberg J.B.M., Bruinenberg P.M., Harder W. 1980; Effect of dissolved oxygen tension on the metabolism of methylated amines in Hyphomicro-bium X in the absence and presence of nitrate: evidence for ‘aerobic’ denitrification. Journal of General Microbiology 120:453–463
    [Google Scholar]
  15. Nicholas D.J.D., Nason A. 1957; Determination of nitrate and nitrite. Methods in Enzymology 3:981–984
    [Google Scholar]
  16. O’Hara G.W., Daniel R.M. 1985; Rhizobial denitrification: a review. Soil Biology and Biochemistry 17:1–9
    [Google Scholar]
  17. O’Hara G.W., Daniel R.M., Steele K.W. 1983; Effect of oxygen on the synthesis, activity and breakdown of the rhizobium denitrification system. Journal of General Microbiology 129:2405–2412
    [Google Scholar]
  18. Payne W.J. 1981 Denitrification. New York: John Wiley & Sons;
    [Google Scholar]
  19. Van Berkum P., Keyser H.H. 1985; Anaerobic growth and denitrification among different sero- groups of soybean rhizobia. Applied and Environmental Microbiology 49:772–777
    [Google Scholar]
  20. Zablotowicz R.M., Eskew D.L., Focht D.D. 1978; Denitrification in Rhizobium. Canadian Journal of Microbiology 24:757–760
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-1-189
Loading
/content/journal/micro/10.1099/00221287-135-1-189
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error