%0 Journal Article %A Moule, Anne L. %A WILKINSON, Stephen G. %T Composition of Lipopolysaccharides from Alteromonas putrefaciens (Shewanella putrefaciens) %D 1989 %J Microbiology, %V 135 %N 1 %P 163-173 %@ 1465-2080 %R https://doi.org/10.1099/00221287-135-1-163 %I Microbiology Society, %X Lipopolysaccharides from eight strains of Alteromonas putrefaciens (Shewanella putrefaciens) representing four groups defined by DNA-DNA homology studies have been compared. All products were of the R-type, lacking a polymeric side chain. In each case lipid A was based on glucosamine and contained a complex range of fatty acids. In general, the major non-hydroxy acids were 11-methyldodecanoic acid and tridecanoic acid, and the major hydroxy acids were 3-hydroxyundecanoic acid, 3-hydroxydodecanoic acid, 3-hydroxy-11-methyldodecanoic acid, 3-hydroxytridecanoic acid and 3-hydroxytetradecanoic acid. All lipopolysaccharides contained a 3-deoxy-2-octulosonic acid, only detectable after dephosphorylation. All core oligosaccharides contained d-galactose and d-glycero-d-manno-heptose, and all except those of group I strains also contained d-galactosamine. Group I strains (including NCIB 10471, the type strain) were distinguished from others by the presence in the core of 3-amino-3,6-dideoxyglucose, d-glucose, and acid-stable phosphate residues. Quinovosamine and d-fructose were found only in group IV strains. Core oligosaccharides from the group II and group III strains, as isolated, were deficient in heptose and seemed to have the same structure. During mild acid hydrolysis of the lipopolysaccharides, various monosaccharides and phosphates were released. The monosaccharides were d-galactose (group I), d-fructose (group IV), and d-galacturonic acid (mainly groups II and III). Phosphates identified were ethanolamine pyrophosphate, ethanolamine phosphate, pyrophosphate, and orthophosphate. The chemotaxonomic implications of the results are discussed. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-135-1-163