The 2-oxoglutarate dehydrogenase complex was isolated from the cellular slime mould, , and purified 113-fold. The enzyme exhibited Michaelis-Menten kinetics and the values for 2-oxoglutarate, CoA, and NAD were 1·0 mM, 0·002 mM, and 0·07 mM, respectively. The value for succinyl-CoA was determined to be 0·004 mM and the for NADH was 0·018 mM. AMP had positive effects whereas ATP had negative effects on the enzyme activity. The kinetic constants determined in this study and the reaction mechanism suggested can now be incorporated into a transition model of the tricarboxylic acid cycle during differentiation of .


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