Summary: At least 14 phosphopolypeptides in which the phosphate groups were present as mono-esters were detected by pulse labelling of subsp. HD-1-Dipel with [P]orthophosphate at different stages of growth and differentiation. Marked changes in the profile of phosphopolypeptides were observed primarily during the late exponential phase of growth. Several phosphopolypeptides co-purified with the endotoxin crystal of this subspecies and the phosphoamino acid residue of the most abundant ( 25000) phosphopolypeptide was identified as phosphothreonine. Comparison of the phosphopolypeptides in endotoxin crystals from several subspecies suggested that 25000 species might be a common component.


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