1887

Abstract

At least 14 phosphopolypeptides in which the phosphate groups were present as mono-esters were detected by pulse labelling of subsp. HD-1-Dipel with [P]orthophosphate at different stages of growth and differentiation. Marked changes in the profile of phosphopolypeptides were observed primarily during the late exponential phase of growth. Several phosphopolypeptides co-purified with the endotoxin crystal of this subspecies and the phosphoamino acid residue of the most abundant ( 25000) phosphopolypeptide was identified as phosphothreonine. Comparison of the phosphopolypeptides in endotoxin crystals from several subspecies suggested that 25000 species might be a common component.

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/content/journal/micro/10.1099/00221287-134-9-2559
1988-09-01
2024-04-16
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References

  1. Antranikian G., Herzberg C., Gottschalk G. 1985; Covalent modification of citrate lyase ligase from Clostridium sphenoides by phosphorylation/dephosphorylation.. European Journal of Biochemistry 153:413–420
    [Google Scholar]
  2. Bulla L.A. Jr Bechtel D.B., Kramer K.J., Shethna Y.I., Aronson A.I., Fitz-James P.C. 1980; Ultrastructure, physiology and biochemistry of Bacillus thuringiensis.. Critical Reviews in Microbiology 8:147–204
    [Google Scholar]
  3. Cortay J.-C., Rleul C., Duclos B., Cozzone A.J. 1986; Characterization of the phospho- proteins of Escherichia coli cells by electrophoretic analysis.. European Journal of Biochemistry 159:227–237
    [Google Scholar]
  4. Deutscher J., Saier M.H. 1983; ATP-dependent protein kinase-catalysed phosphorylation of a seryl residue in HPr, a phosphate carrier protein of the phosphotransferase system in Streptococcus pyogenes.. Proceedings of the National Academy of Sciences ofithe United States of America 80:6790–6794
    [Google Scholar]
  5. Holmes K.C., Monro R.E. 1965; Studies on the structure of parasporal inclusions from Bacillus thuringiensis.. Journal of Molecular Biology 14:572–581
    [Google Scholar]
  6. Kalbitzer H.R., Hengstenberg W., Rosch P., Muss P., Bernsman P., Engelmann R., Dorschug M., Deutscher J. 1982; HPr proteins of different microorganisms studied by hydrogen-1 high resolution nuclear magnetic resonance: similarities of structures and mechanisms.. Biochemistry 21:2879–2885
    [Google Scholar]
  7. Komano T., Brown N., Inouye S., Inouye M. 1982; Phosphorylation and methylation of proteins during Myxococcus xanthus spore formation.. Journal of Bacteriology 151:114–118
    [Google Scholar]
  8. Manai M., Cozzone A.J. 1982; Endogenous protein phosphorylation in Escherichia coli extracts.. Biochemical and Biophysical Research Communications 107:981–988
    [Google Scholar]
  9. Mann N.H., Turner A.M. 1988; Covalent modification of ribulose-l,5-bisphosphate carboxylase/oxygenase in Rhodomicrobium vamielii.. Molecular Microbiology 2:427–432
    [Google Scholar]
  10. Nimmo H.G. 1984; Control of Escherichia coli isocitrate dehydrogenase: an example of protein phosphorylation in a prokaryote.. Trends in Biochemical Sciences 9:478–480
    [Google Scholar]
  11. Reizer J., Deutscher J., Sutrina S., Thompson J., Saier M.H. Jr 1985; Sugar accumulation in Gram positive bacteria: exclusion and expulsion mechanisms.. Trends in Biochemical Sciences 10:32–35
    [Google Scholar]
  12. Sharpe E.S., Nickerson K.W., Bulla L.A. Jr Aronson J.N. 1975; Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain X-ray contrasting agents.. Applied Microbiology 30:1052–1053
    [Google Scholar]
  13. Singer S., Rogoff M.H. 1968; Inhibition of growth of Bacillus thuringiensis by amino acids in defined media.. Journal of Invertebrate Pathology 12:98–104
    [Google Scholar]
  14. Turner A.M., Mann N.H. 1986; Protein phosphorylation in Rhodomicrobium vannielii.. Journal of General Microbiology 132:3433–3400
    [Google Scholar]
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