Summary: A partial 3 AI genomic bank of DSM 760 was constructed in the cosmid pSUP106 and , the structural gene for methanol dehydrogenase, was isolated. In , pSUP106 behaves as a suicide plasmid. This property was used to insert Tn5 into the bacterial chromosome, in the vicinity of , by marker exchange. Mobilization of the Tn5-labelled chromosomal region by a broad-host-range plasmid, pJB3J1 (an R68-45 derivative), allowed the selection of several large R” hybrid plasmids in HB101. Most of them were able to complement both mutants of the region and mutant MTM1, the first mutant of the pyrroloquinoline quinone (PQQ) biosynthesis pathway in The gene involved, , was subcloned and localized.


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